##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546966_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2859705
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547768738383855	31.0	31.0	33.0	30.0	34.0
2	31.904430701768188	31.0	31.0	34.0	30.0	34.0
3	31.738670247455595	31.0	31.0	34.0	30.0	34.0
4	35.59919397280489	37.0	35.0	37.0	33.0	37.0
5	35.606528295750785	37.0	35.0	37.0	33.0	37.0
6	35.68485420698988	37.0	35.0	37.0	33.0	37.0
7	36.105049996415715	37.0	35.0	37.0	35.0	37.0
8	36.05455213037708	37.0	35.0	37.0	35.0	37.0
9	37.81880683497074	39.0	38.0	39.0	35.0	39.0
10	37.246839796412566	39.0	37.0	39.0	34.0	39.0
11	37.078523134379246	39.0	37.0	39.0	33.0	39.0
12	36.84613238078753	39.0	35.0	39.0	33.0	39.0
13	36.803417135683574	39.0	35.0	39.0	33.0	39.0
14	37.9822670520211	40.0	37.0	41.0	33.0	41.0
15	38.03805357545621	40.0	37.0	41.0	33.0	41.0
16	38.082084340867326	40.0	36.0	41.0	33.0	41.0
17	38.03783607050378	40.0	36.0	41.0	33.0	41.0
18	37.790573153524576	39.0	36.0	41.0	33.0	41.0
19	37.555820268174514	39.0	36.0	41.0	33.0	41.0
20	37.23075527021144	39.0	35.0	41.0	33.0	41.0
21	37.18119596252061	39.0	35.0	41.0	33.0	41.0
22	37.1093217657066	38.0	35.0	41.0	33.0	41.0
23	37.020599327553015	38.0	35.0	41.0	32.0	41.0
24	36.912843107942955	38.0	35.0	41.0	32.0	41.0
25	36.81349614732988	38.0	35.0	41.0	32.0	41.0
26	36.66434614759215	38.0	35.0	41.0	32.0	41.0
27	36.57441729129403	38.0	35.0	40.0	31.0	41.0
28	36.53612173283608	38.0	35.0	40.0	31.0	41.0
29	36.481315730119015	38.0	35.0	41.0	31.0	41.0
30	36.323144170465135	38.0	35.0	40.0	31.0	41.0
31	36.04992682811689	38.0	35.0	40.0	30.0	41.0
32	35.62259288982605	38.0	35.0	41.0	27.0	41.0
33	35.08162590197241	38.0	34.0	41.0	23.0	41.0
34	34.5471676274301	38.0	34.0	41.0	21.0	41.0
35	34.16283917397074	38.0	34.0	41.0	18.0	41.0
36	33.919946637852505	38.0	33.0	41.0	15.0	41.0
37	33.795560381228135	38.0	33.0	40.0	15.0	41.0
38	33.667545078950454	38.0	33.0	40.0	15.0	41.0
39	33.5666598477815	38.0	33.0	40.0	13.0	41.0
40	33.46456085505323	38.0	33.0	40.0	12.0	41.0
41	33.36062740737244	38.0	33.0	40.0	12.0	41.0
42	33.265481929080096	37.0	33.0	40.0	10.0	41.0
43	33.14510133038198	37.0	33.0	40.0	10.0	41.0
44	32.993507022577504	37.0	32.0	40.0	10.0	41.0
45	32.88591340715214	37.0	32.0	40.0	10.0	41.0
46	32.79936741726856	36.0	32.0	40.0	10.0	41.0
47	32.69564692861676	36.0	32.0	40.0	10.0	41.0
48	32.5147639354409	36.0	32.0	40.0	10.0	41.0
49	32.389387017192334	36.0	31.0	40.0	10.0	41.0
50	32.23396679028082	35.0	31.0	40.0	10.0	41.0
51	31.876714905908127	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	5.0
12	4.0
13	6.0
14	15.0
15	76.0
16	235.0
17	643.0
18	1561.0
19	3195.0
20	5668.0
21	9329.0
22	14653.0
23	23241.0
24	38854.0
25	65436.0
26	90559.0
27	89349.0
28	75103.0
29	67235.0
30	68514.0
31	76327.0
32	90217.0
33	111159.0
34	167835.0
35	270905.0
36	213447.0
37	254430.0
38	376326.0
39	744783.0
40	594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.744538335247867	22.90970572139434	25.63704997543453	22.708705967923265
2	40.38402562502076	23.801161308596516	24.343734755857685	11.471078310525035
3	25.250436670915356	22.834628047298587	39.299997727038274	12.61493755474778
4	21.07874763306005	26.04974988678902	39.488268894868526	13.383233585282397
5	19.741721611145206	29.70862379161487	37.958181001187185	12.59147359605274
6	17.615138624438533	37.74221466899558	35.03973311932525	9.602913587240643
7	77.83180432946754	2.8997046898194045	17.38889151153703	1.8795994691760163
8	77.6935033508701	2.505013629028169	16.90538010039497	2.896102919706753
9	72.3360976044732	4.749790625256801	19.4939687834934	3.4201429867766078
10	31.63137456485896	24.147910361383428	29.31270882835817	14.908006245399438
11	25.80969016034871	25.456576814741382	32.936474216746134	15.79725880816378
12	21.91267980438542	21.654541290098102	38.10424501827986	18.32853388723662
13	19.44441821796304	26.662750178777184	39.093542865435424	14.799288737824357
14	17.29709882662722	29.46884381430952	37.86642328491925	15.367634074144013
15	16.090715650740197	25.75860097457605	43.350590358096376	14.800093016587375
16	17.10931721978316	23.70157061654961	42.857252758588736	16.331859405078497
17	16.840618175650985	25.150321449240394	37.302553934759004	20.706506440349617
18	18.525022685906414	24.90942247539519	39.079975032389704	17.485579806308692
19	20.33157266221516	28.34023089794227	34.489571476778195	16.838624963064373
20	21.020140189285257	25.855743861692027	34.95343051118909	18.170685437833622
21	19.05430804925683	28.3776473447436	35.92965008628512	16.638394519714446
22	18.79907193224476	25.421957859289684	35.31074009382086	20.46823011464469
23	16.70595393580806	28.93927870182414	34.320008532348616	20.034758830019182
24	17.13190696243144	26.649182345731464	39.08745832174997	17.131452370087125
25	17.924506199065988	27.223122664750388	36.68339916180165	18.16897197438197
26	16.7786887108985	29.807235361689404	35.34745716778479	18.066618759627303
27	18.094628641765496	28.379780431897693	35.06526022789064	18.460330698446167
28	17.7430189477586	27.791363095144426	37.538662204667965	16.92695575242901
29	18.340178444979465	25.81587960995977	36.33392255494885	19.510019390111918
30	19.87572144679259	27.321454485689955	34.98151033061102	17.82131373690643
31	21.22788889063732	27.781571875420717	32.78219256881392	18.208346665128047
32	22.98432880314578	26.81185646771258	32.58475961681363	17.61905511232802
33	21.98037909504652	28.051494822018352	31.010716140301188	18.957409942633944
34	20.721228238577055	27.437830125834655	31.06030167447342	20.78063996111487
35	21.41969189129648	27.740343846655513	30.864477279999157	19.97548698204885
36	23.18162188057859	27.63795566325897	29.253192199894745	19.92723025626769
37	21.207082548724433	30.062996008329527	30.21133298714378	18.51858845580226
38	20.547189307988063	29.973091630080724	30.150242769796186	19.329476292135027
39	20.54239860405182	29.823460811517272	29.052192446423668	20.58194813800724
40	21.63429444645514	27.92137650561859	30.06628306066535	20.378045987260922
41	18.79830262212361	28.056810055582655	30.779643354821562	22.36524396747217
42	20.59313810340577	29.473984204664465	29.245988659669443	20.686889032260318
43	20.068014008437935	28.548119473861817	31.038236461453188	20.34563005624706
44	20.872467614666547	29.85919876350882	29.054150690368413	20.214182931456218
45	19.491695821771827	29.949662640027558	28.978653392570212	21.579988145630406
46	21.45266732058027	29.19748715339519	28.53483138995106	20.815014136073476
47	20.907785942955652	28.38264786053107	29.836224365799968	20.873341830713308
48	20.514039035494918	27.354045259913175	30.684738460785294	21.447177243806617
49	20.497813585667053	26.631488212945044	30.324631386803887	22.54606681458402
50	19.5738371615254	27.90515105579072	31.179999335595802	21.341012447088072
51	19.020947964912466	28.603684645793887	29.562105182177884	22.81326220711577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2327.0
1	2573.0
2	2819.0
3	45188.0
4	87557.0
5	58568.5
6	29580.0
7	30551.5
8	31523.0
9	35328.0
10	39133.0
11	41932.5
12	44732.0
13	46291.5
14	47851.0
15	47303.5
16	46756.0
17	45182.0
18	43608.0
19	41470.0
20	39332.0
21	37080.0
22	34828.0
23	34519.0
24	34210.0
25	32409.5
26	32260.5
27	33912.0
28	36138.0
29	38364.0
30	41791.0
31	45218.0
32	50611.5
33	56005.0
34	64019.5
35	72034.0
36	79326.0
37	86618.0
38	96174.5
39	105731.0
40	119489.5
41	133248.0
42	150057.0
43	166866.0
44	178550.5
45	190235.0
46	198059.5
47	205884.0
48	231643.5
49	257403.0
50	262278.0
51	267153.0
52	243056.0
53	218959.0
54	185889.0
55	152819.0
56	128935.0
57	105051.0
58	88610.5
59	72170.0
60	61039.5
61	49909.0
62	41256.0
63	32603.0
64	27817.0
65	23031.0
66	18152.0
67	13273.0
68	10165.0
69	7057.0
70	5665.5
71	4274.0
72	3421.5
73	2569.0
74	2077.0
75	1426.5
76	1268.0
77	1016.0
78	764.0
79	591.0
80	418.0
81	290.5
82	163.0
83	121.5
84	80.0
85	76.5
86	73.0
87	64.0
88	55.0
89	42.0
90	29.0
91	18.5
92	8.0
93	6.5
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2859705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.52104473711347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39113700707838	25.942317658703523
2	8.17738188730407	5.482287681535623
3	3.364290440824949	3.38323591126609
4	1.9001627647772417	2.5478176418358056
5	1.2824002352218147	2.1493697827877645
6	0.8786487122055965	1.7671933674030564
7	0.7294156066676901	1.7115541228139484
8	0.567411668172827	1.5216185530545214
9	0.48997958950271464	1.4782164965993692
>10	4.544814469343222	32.071771329924275
>50	0.588695792703884	13.364840950219595
>100	0.08217682595054512	4.06928811632538
>500	0.0014784849533071092	0.34741812544331446
>1k	0.0015840910214002671	1.1393926824452263
>5k	3.168182042800534E-4	0.710978768229352
>10k+	1.0560606809335114E-4	2.312698811413243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65330	2.2845013733934096	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	8029	0.2807632255774634	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	6474	0.22638698746898717	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	5581	0.19515999027871755	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	4886	0.17085678417878766	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3343	0.11690016977275629	TruSeq Adapter, Index 14 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	3287	0.11494192582801374	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.993728374080543E-5	0.0	0.0	0.1030875562339472	0.0
2	6.993728374080543E-5	0.0	0.0	0.3383216100961463	0.0
3	6.993728374080543E-5	0.0	0.0	0.6852105374505413	0.0
4	6.993728374080543E-5	0.0	0.0	1.0439188657571323	0.0
5	6.993728374080543E-5	0.0	0.0	1.7371372221959958	0.0
6	6.993728374080543E-5	0.0	0.0	3.1857132116774283	0.0
7	6.993728374080543E-5	0.0	0.0	3.997300420847605	0.0
8	6.993728374080543E-5	0.0	0.0	5.49039149143006	0.0
9	6.993728374080543E-5	0.0	0.0	6.3784900890126774	0.0
10	6.993728374080543E-5	0.0	0.0	7.511998615241782	0.0
11	6.993728374080543E-5	0.0	0.0	8.40278979824842	0.0
12	6.993728374080543E-5	0.0	0.0	9.16339272757155	0.0
13	6.993728374080543E-5	0.0	0.0	9.670123316915555	0.0
14	6.993728374080543E-5	0.0	0.0	9.936304618833061	0.0
15	6.993728374080543E-5	0.0	0.0	10.153320010280781	0.0
16	6.993728374080543E-5	0.0	0.0	10.49457898629404	0.0
17	6.993728374080543E-5	0.0	0.0	10.853147440033151	0.0
18	6.993728374080543E-5	0.0	0.0	11.325259073925457	0.0
19	6.993728374080543E-5	0.0	0.0	11.572137685530501	0.0
20	6.993728374080543E-5	0.0	0.0	11.85174694592624	0.0
21	1.3987456748161087E-4	0.0	0.0	12.154295635388966	0.0
22	1.7484320935201358E-4	0.0	0.0	12.493001900545686	0.0
23	1.7484320935201358E-4	0.0	0.0	12.880804138888452	0.0
24	1.7484320935201358E-4	0.0	0.0	13.19237473795374	0.0
25	1.7484320935201358E-4	0.0	0.0	13.469151538357977	0.0
26	1.7484320935201358E-4	0.0	0.0	13.7264508052404	0.0
27	1.7484320935201358E-4	0.0	0.0	14.00088470663932	0.0
28	1.7484320935201358E-4	0.0	0.0	14.277451695192337	0.0
29	1.7484320935201358E-4	0.0	0.0	14.569334948884588	0.0
30	1.7484320935201358E-4	0.0	0.0	14.963081856345323	0.0
31	1.7484320935201358E-4	0.0	0.0	15.266434824571066	0.0
32	1.7484320935201358E-4	0.0	0.0	15.54377112324523	0.0
33	1.7484320935201358E-4	0.0	0.0	15.83806721322654	0.0
34	1.7484320935201358E-4	0.0	0.0	16.135475512334313	0.0
35	1.7484320935201358E-4	0.0	0.0	16.448374919790677	0.0
36	1.7484320935201358E-4	0.0	0.0	16.746657434945213	0.0
37	1.7484320935201358E-4	0.0	0.0	17.065641386087027	0.0
38	1.7484320935201358E-4	0.0	0.0	17.39249328164968	0.0
39	1.7484320935201358E-4	0.0	0.0	17.728052369038064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACGT	30	2.1666401E-6	45.000004	40
TACGACC	25	3.892267E-5	45.0	28
CGTTTTT	38730	0.0	44.37839	1
TGTTACG	90	0.0	42.5	1
TAATGCG	50	1.0822987E-9	40.5	1
CGACGGT	95	0.0	40.263157	28
GTCGACG	85	0.0	39.705883	1
TACGACG	40	3.4606273E-7	39.375	1
GCGTAAG	200	0.0	39.375	1
GTTTTTT	43795	0.0	39.333256	2
CGGTTTT	1100	0.0	38.454544	1
GTTAGCG	135	0.0	38.333332	1
TACGGGT	540	0.0	38.333332	4
CGTTAGG	370	0.0	38.31081	2
AGGGCGA	2960	0.0	37.930744	6
GGACCGA	1550	0.0	37.887096	8
GGGACCG	1690	0.0	37.810654	7
CGTTCTG	685	0.0	37.77372	1
ATTAGCG	120	0.0	37.500004	1
TATTACG	120	0.0	37.500004	1
>>END_MODULE