FastQCFastQC Report
Thu 26 May 2016
SRR1546965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546965_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1531168
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200781.3112865472632658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121750.042.9301831
CTACGGG2200.039.8863683
GGGCGAT36850.038.710997
GCGATAC1050.038.571439
TACGGGA3100.038.467744
GGCGATA6750.038.3333328
TATTGCG1000.038.2500041
TAGGGAC8800.037.5852285
AGGGCGA19700.037.1192866
TTGATCC9550.036.9895317
GTAGCAT9550.036.7539329
GTTTTTT147450.036.6988832
GACCGAT11400.036.315799
GCCAGTA9550.036.28272225
GTTGATC9700.036.18556616
CACGACC8650.036.1560727
CATATGC9600.036.09375433
TAGCATA9550.036.04712330
TCACGAC504.8807124E-836.00000425
TACGGGT1000.036.0000044
Produced by FastQC (version 0.11.5)