##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546965_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1531168
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.588361956362725	31.0	31.0	33.0	30.0	34.0
2	31.967423561620933	31.0	31.0	34.0	30.0	34.0
3	31.96527356893561	33.0	31.0	34.0	30.0	34.0
4	35.73741548935192	37.0	35.0	37.0	33.0	37.0
5	35.70433877928483	37.0	35.0	37.0	33.0	37.0
6	35.76363142385421	37.0	35.0	37.0	33.0	37.0
7	36.08770559468327	37.0	35.0	37.0	35.0	37.0
8	36.02035047754394	37.0	35.0	37.0	35.0	37.0
9	37.79568473217831	39.0	38.0	39.0	35.0	39.0
10	37.34794548997889	39.0	37.0	39.0	34.0	39.0
11	37.07957193462768	39.0	37.0	39.0	33.0	39.0
12	36.7122719388075	39.0	35.0	39.0	33.0	39.0
13	36.63408522121674	39.0	35.0	39.0	33.0	39.0
14	37.70877330247236	40.0	36.0	41.0	33.0	41.0
15	37.77173896006186	40.0	36.0	41.0	33.0	41.0
16	37.823217961712885	40.0	36.0	41.0	33.0	41.0
17	37.744049640535856	40.0	36.0	41.0	33.0	41.0
18	37.56524039164873	39.0	36.0	41.0	32.0	41.0
19	37.43108006436916	39.0	36.0	41.0	32.0	41.0
20	37.220485276599305	39.0	35.0	41.0	32.0	41.0
21	37.159282325649436	39.0	35.0	41.0	32.0	41.0
22	37.09611682062321	39.0	35.0	41.0	32.0	41.0
23	36.99486535768773	39.0	35.0	41.0	32.0	41.0
24	36.8572579886727	38.0	35.0	41.0	32.0	41.0
25	36.73167346757508	38.0	35.0	41.0	31.0	41.0
26	36.5793093899559	38.0	35.0	41.0	31.0	41.0
27	36.46617941336287	38.0	35.0	41.0	31.0	41.0
28	36.469794300821334	38.0	35.0	41.0	31.0	41.0
29	36.4275487732241	38.0	35.0	41.0	31.0	41.0
30	36.297577404961444	38.0	35.0	40.0	30.0	41.0
31	36.13267388033188	38.0	35.0	40.0	30.0	41.0
32	35.91724487450104	38.0	35.0	41.0	30.0	41.0
33	35.62069740224456	38.0	35.0	41.0	27.0	41.0
34	35.25490409935422	38.0	34.0	41.0	25.0	41.0
35	35.026744942423036	38.0	34.0	41.0	24.0	41.0
36	34.88503221070451	38.0	34.0	40.0	23.0	41.0
37	34.802623226190725	38.0	34.0	40.0	23.0	41.0
38	34.67656716963782	38.0	34.0	40.0	23.0	41.0
39	34.549962512278206	37.0	34.0	40.0	23.0	41.0
40	34.39847227737257	37.0	34.0	40.0	22.0	41.0
41	34.283657965683716	37.0	34.0	40.0	21.0	41.0
42	34.181740997722	37.0	33.0	40.0	21.0	41.0
43	34.049690171163455	37.0	33.0	40.0	21.0	41.0
44	33.89283409789128	36.0	33.0	40.0	20.0	41.0
45	33.77440228635917	36.0	33.0	40.0	20.0	41.0
46	33.71523111768271	36.0	33.0	40.0	20.0	41.0
47	33.64880078476039	36.0	33.0	40.0	20.0	41.0
48	33.532416429810446	35.0	33.0	40.0	20.0	41.0
49	33.41932302660452	35.0	33.0	40.0	20.0	41.0
50	33.31080913394219	35.0	33.0	40.0	20.0	41.0
51	32.93681033041443	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	4.0
14	20.0
15	57.0
16	155.0
17	391.0
18	839.0
19	1593.0
20	2656.0
21	4316.0
22	6561.0
23	9526.0
24	14448.0
25	22074.0
26	31192.0
27	35854.0
28	36406.0
29	36598.0
30	38650.0
31	43677.0
32	51096.0
33	65373.0
34	109249.0
35	147442.0
36	120994.0
37	154752.0
38	218948.0
39	378058.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22176273276349	23.432895671800875	25.03546312357625	18.309878471859392
2	37.098803005287465	23.930032498066836	25.846477982820957	13.12468651382474
3	28.848761207130764	24.47549844301866	33.409985057158984	13.265755292691592
4	25.45279159439069	27.10943541139836	33.0379814625175	14.39979153169345
5	22.947579886726995	30.59305053397145	31.931114025371482	14.528255553930073
6	21.650073669251185	39.40109772409037	29.00667986791782	9.942148738740622
7	83.53949403331313	3.25947250726243	11.506183514806997	1.6948499446174423
8	83.15168551066898	2.9394553700181825	11.370861982486574	2.537997136826266
9	77.97655123409058	4.454899788919309	13.9410567618968	3.627492215093314
10	43.10630838680014	24.020551631173063	19.63879861648101	13.23434136554578
11	32.810312127735166	24.987395243369768	26.36177088340404	15.840521745491024
12	28.54494085560827	20.71660327279567	31.38512560346089	19.353330268135174
13	24.505410248908024	22.637163263600073	33.800797822316035	19.056628665175865
14	22.07889663315848	24.047067336830448	34.48125875148906	19.392777278522015
15	21.885384229555477	21.574967606428558	37.68796108591611	18.851687078099854
16	25.723304039791845	20.44524180233652	34.63480166774645	19.196652490125185
17	25.22682030972434	21.172660348178646	31.955735752053332	21.64478359004368
18	25.523260674204266	21.513903111872768	32.812924512528994	20.149911701393968
19	27.376747685427073	24.277022508307383	29.033979289013356	19.31225051725219
20	28.438943342598595	24.142745929904493	28.416084975652574	19.002225751844342
21	27.368192125227274	23.991292921482163	29.758067044243347	18.88244790904721
22	25.563817948128488	21.835161131894086	30.428862149679198	22.172158770298232
23	24.937694622667138	23.796017158143325	29.59015601161989	21.676132207569644
24	24.677958264540536	22.44913686806412	32.456595226650506	20.416309640744842
25	25.27541066688959	22.559183577504232	30.212230140650796	21.95317561495538
26	22.527834959978264	23.927093565173777	30.311566072436207	23.23350540241175
27	22.794624757048215	22.646698468097558	30.956759806892514	23.601916967961714
28	22.62553815126753	24.565233860686746	31.1743714602186	21.63485652782712
29	24.553347509874815	23.086101590419865	30.35127432130243	22.009276578402893
30	25.5875253401325	22.467815419340006	31.254898221488432	20.68976101903906
31	26.629409705531987	22.972854709607308	28.574656732638093	21.823078852222615
32	28.437702459821523	22.443520240757383	27.91881753014692	21.199959769274173
33	26.655598863090134	23.208426508390982	27.88152573721499	22.254448891303895
34	24.16109793308115	24.793099124328617	29.871771092394827	21.17403185019541
35	23.735540450166148	25.582431189784533	29.47024754958306	21.21178081046626
36	25.64120984764572	27.168867165458003	26.01020919977429	21.179713787121987
37	24.316665447553763	27.267288762565574	27.134840853518362	21.281204936362304
38	25.03454878889841	26.891170661873808	26.64430029885682	21.42998025037096
39	24.36316589688395	26.089103220547972	26.430280674622246	23.11745020794583
40	25.40818512403603	24.766714037910926	27.68304980250371	22.142051035549333
41	22.286646533887854	25.307085832514787	27.81993876570043	24.586328867896924
42	24.01323695375034	25.591574536562938	26.69151915400531	23.703669355681413
43	23.22723567890656	23.896071495747037	28.782145394888087	24.094547430458316
44	23.623142594411586	24.483662145499384	28.067070367196806	23.826124892892224
45	23.070296662417185	24.5882881564923	28.117097535998663	24.22431764509185
46	23.82403498505716	24.778339150243475	27.805440030094676	23.592185834604695
47	22.853207486049865	25.137215511295953	29.042796087692537	22.966780914961653
48	23.45007210182031	24.097355744111685	29.33120336893143	23.121368785136575
49	24.08416320090284	23.898226713201947	28.553561725427905	23.464048360467306
50	22.914141361365964	24.380603565382767	29.416105874730924	23.289149198520345
51	22.316362410917677	24.987264624130077	28.88775105017869	23.80862191477356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	911.0
1	1728.5
2	2546.0
3	18519.5
4	34493.0
5	23815.5
6	13138.0
7	12856.0
8	12574.0
9	12842.5
10	13111.0
11	13032.0
12	12953.0
13	12732.0
14	12511.0
15	12028.0
16	11545.0
17	10867.0
18	10189.0
19	9829.0
20	9469.0
21	9012.0
22	8555.0
23	8345.5
24	8136.0
25	8683.5
26	10295.0
27	11359.0
28	12650.0
29	13941.0
30	15326.0
31	16711.0
32	19706.0
33	22701.0
34	26048.5
35	29396.0
36	31417.5
37	33439.0
38	37663.5
39	41888.0
40	46646.0
41	51404.0
42	57750.5
43	64097.0
44	70167.0
45	76237.0
46	84174.5
47	92112.0
48	99150.5
49	106189.0
50	105934.5
51	105680.0
52	100846.0
53	96012.0
54	91991.0
55	87970.0
56	87552.0
57	87134.0
58	86629.0
59	86124.0
60	83649.5
61	81175.0
62	75675.0
63	70175.0
64	63323.5
65	56472.0
66	48859.5
67	41247.0
68	35871.5
69	30496.0
70	26884.0
71	23272.0
72	20329.0
73	17386.0
74	14970.5
75	10315.5
76	8076.0
77	6100.0
78	4124.0
79	3165.5
80	2207.0
81	1689.5
82	1172.0
83	901.5
84	631.0
85	439.5
86	248.0
87	173.5
88	99.0
89	65.5
90	32.0
91	31.5
92	31.0
93	21.5
94	12.0
95	7.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1531168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.627676791794034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89716664158051	22.4231267199387
2	6.582224600810141	4.1636094449083725
3	3.3252936365165326	3.15513937120663
4	2.3613161642378024	2.9873177778300817
5	1.9331813472244246	3.0571017414969526
6	1.6805300291334864	3.1890756360202874
7	1.4644198127009447	3.2421337566523523
8	1.3475472758684688	3.4095831762264357
9	1.2292822825376688	3.4991408446182213
>10	9.093452431880644	46.42232206315509
>50	0.061674622349036506	1.2836052382017828
>100	0.02120816239012375	1.3139741069291433
>500	0.0022871477286065286	0.4628263603411993
>1k	2.079225207824117E-4	0.07069314256419285
>5k	0.0	0.0
>10k+	2.079225207824117E-4	1.3203506199105712
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20078	1.3112865472632658	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.306192396915296E-4	0.0	0.0	0.060215469497795146	0.0
2	1.306192396915296E-4	0.0	0.0	0.1575268030679847	0.0
3	1.306192396915296E-4	0.0	0.0	0.257972998390771	0.0
4	1.306192396915296E-4	0.0	0.0	0.42751677151037637	0.0
5	1.306192396915296E-4	0.0	0.0	0.7055398231937972	0.0
6	1.306192396915296E-4	0.0	0.0	1.3829312002340697	0.0
7	1.306192396915296E-4	0.0	0.0	1.7579390373884511	0.0
8	1.306192396915296E-4	0.0	0.0	2.6522889715563545	0.0
9	1.306192396915296E-4	0.0	0.0	3.2195683295366675	0.0
10	1.306192396915296E-4	0.0	0.0	3.906103053355347	0.0
11	1.306192396915296E-4	0.0	0.0	4.351841208802692	0.0
12	1.306192396915296E-4	0.0	0.0	4.677017806014755	0.0
13	1.306192396915296E-4	0.0	0.0	4.879281698677088	0.0
14	1.306192396915296E-4	0.0	0.0	5.019240214006563	0.0
15	1.306192396915296E-4	0.0	0.0	5.121057911346109	0.0
16	1.959288595372944E-4	0.0	0.0	5.266763673222011	0.0
17	1.959288595372944E-4	0.0	0.0	5.4098570503040815	0.0
18	1.959288595372944E-4	0.0	0.0	5.615974210537315	0.0
19	1.959288595372944E-4	0.0	0.0	5.735686743714602	0.0
20	1.959288595372944E-4	0.0	0.0	5.865979435306903	0.0
21	1.959288595372944E-4	0.0	0.0	6.006982904553909	0.0
22	1.959288595372944E-4	0.0	0.0	6.167122092415725	0.0
23	1.959288595372944E-4	0.0	0.0	6.3452867353549705	0.0
24	1.959288595372944E-4	0.0	0.0	6.498503103513135	0.0
25	1.959288595372944E-4	0.0	0.0	6.635326757090012	0.0
26	1.959288595372944E-4	0.0	0.0	6.7666644025998455	0.0
27	1.959288595372944E-4	0.0	0.0	6.913349808773433	0.0
28	1.959288595372944E-4	0.0	0.0	7.04880196033355	0.0
29	1.959288595372944E-4	0.0	0.0	7.205610357583231	0.0
30	1.959288595372944E-4	0.0	0.0	7.4185197182804234	0.0
31	1.959288595372944E-4	0.0	0.0	7.573172898075195	0.0
32	1.959288595372944E-4	0.0	0.0	7.75551735668457	0.0
33	1.959288595372944E-4	0.0	0.0	7.929045016614768	0.0
34	1.959288595372944E-4	0.0	0.0	8.117397960249953	0.0
35	1.959288595372944E-4	0.0	0.0	8.316265752680307	0.0
36	1.959288595372944E-4	0.0	0.0	8.495410039917239	0.0
37	1.959288595372944E-4	0.0	0.0	8.682979268114277	0.0
38	1.959288595372944E-4	0.0	0.0	8.923383978766536	0.0
39	1.959288595372944E-4	0.0	0.0	9.262014357666827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12175	0.0	42.930183	1
CTACGGG	220	0.0	39.886368	3
GGGCGAT	3685	0.0	38.71099	7
GCGATAC	105	0.0	38.57143	9
TACGGGA	310	0.0	38.46774	4
GGCGATA	675	0.0	38.333332	8
TATTGCG	100	0.0	38.250004	1
TAGGGAC	880	0.0	37.585228	5
AGGGCGA	1970	0.0	37.119286	6
TTGATCC	955	0.0	36.98953	17
GTAGCAT	955	0.0	36.75393	29
GTTTTTT	14745	0.0	36.698883	2
GACCGAT	1140	0.0	36.31579	9
GCCAGTA	955	0.0	36.282722	25
GTTGATC	970	0.0	36.185566	16
CACGACC	865	0.0	36.15607	27
CATATGC	960	0.0	36.093754	33
TAGCATA	955	0.0	36.047123	30
TCACGAC	50	4.8807124E-8	36.000004	25
TACGGGT	100	0.0	36.000004	4
>>END_MODULE