FastQCFastQC Report
Thu 26 May 2016
SRR1546964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546964_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences256142
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18380.7175707224898689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG302.158873E-645.0000042
ACAACGA406.7793735E-945.013
ACCGGCC253.8822895E-545.021
CTATATG253.8822895E-545.01
CGTTAGG502.1827873E-1145.02
GCCGATA253.8822895E-545.09
GCGATAA253.8822895E-545.09
CACAACG406.7793735E-945.012
TAGTGCG253.8822895E-545.01
TATATGG800.042.18752
CGTTTTT11250.042.01
GTCAAGC1300.041.5384616
GGTATGC603.6379788E-1241.2500048
AACACGT1100.040.90909241
CGACCCG501.0750227E-940.532
TAGTAGG1000.040.52
GGGCGAT5750.040.3043487
GGTACCT1850.040.1351368
ACACGAC1350.040.00000426
CGACCAA1350.040.00000429
Produced by FastQC (version 0.11.5)