##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546964_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	256142
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60903327060771	31.0	31.0	33.0	30.0	34.0
2	32.00438819092535	31.0	31.0	34.0	30.0	34.0
3	32.09266344449563	33.0	31.0	34.0	30.0	34.0
4	35.800462243599256	37.0	35.0	37.0	35.0	37.0
5	35.75291049495983	37.0	35.0	37.0	35.0	37.0
6	35.802051205971686	37.0	35.0	37.0	35.0	37.0
7	36.0916991356357	37.0	35.0	37.0	35.0	37.0
8	36.019496997759056	37.0	35.0	37.0	35.0	37.0
9	37.787231301387514	39.0	38.0	39.0	35.0	39.0
10	37.36269334978254	39.0	37.0	39.0	34.0	39.0
11	37.077753745969034	39.0	37.0	39.0	33.0	39.0
12	36.67076855806545	39.0	35.0	39.0	33.0	39.0
13	36.55789757244029	39.0	35.0	39.0	33.0	39.0
14	37.652298334517575	40.0	36.0	41.0	33.0	41.0
15	37.73414356099351	40.0	36.0	41.0	33.0	41.0
16	37.793684752988575	40.0	36.0	41.0	33.0	41.0
17	37.73326514199155	40.0	36.0	41.0	33.0	41.0
18	37.610946271989754	39.0	36.0	41.0	33.0	41.0
19	37.51185670448423	39.0	36.0	41.0	33.0	41.0
20	37.37328513090395	39.0	35.0	41.0	32.0	41.0
21	37.29410249002506	39.0	35.0	41.0	32.0	41.0
22	37.20994994963731	39.0	35.0	41.0	32.0	41.0
23	37.11155140507999	39.0	35.0	41.0	32.0	41.0
24	36.97756713073217	39.0	35.0	41.0	32.0	41.0
25	36.83063691233769	38.0	35.0	41.0	31.0	41.0
26	36.67451647914048	38.0	35.0	41.0	31.0	41.0
27	36.5799985945296	38.0	35.0	41.0	31.0	41.0
28	36.592866456887194	38.0	35.0	41.0	31.0	41.0
29	36.563632672501974	38.0	35.0	41.0	31.0	41.0
30	36.46694021285069	38.0	35.0	41.0	31.0	41.0
31	36.328712198702284	38.0	35.0	41.0	30.0	41.0
32	36.17010095962396	38.0	35.0	41.0	30.0	41.0
33	36.00131567646071	38.0	35.0	41.0	30.0	41.0
34	35.74789765052198	38.0	35.0	41.0	29.0	41.0
35	35.569477086928345	38.0	35.0	41.0	27.0	41.0
36	35.443601595989726	38.0	35.0	41.0	27.0	41.0
37	35.33247964019958	38.0	35.0	40.0	27.0	41.0
38	35.186033528277285	38.0	34.0	40.0	26.0	41.0
39	35.07726183132794	38.0	34.0	40.0	26.0	41.0
40	34.91255241233378	37.0	34.0	40.0	25.0	41.0
41	34.809859374877995	37.0	34.0	40.0	24.0	41.0
42	34.69800735529511	37.0	34.0	40.0	24.0	41.0
43	34.57041016311265	37.0	34.0	40.0	24.0	41.0
44	34.40337781386887	36.0	34.0	40.0	23.0	41.0
45	34.30123525232098	36.0	34.0	40.0	23.0	41.0
46	34.24683964363517	36.0	34.0	40.0	23.0	41.0
47	34.18805193993956	36.0	33.0	40.0	23.0	41.0
48	34.06830195750794	35.0	33.0	40.0	23.0	41.0
49	33.95992847717282	35.0	33.0	40.0	23.0	41.0
50	33.85357731258443	35.0	33.0	40.0	23.0	41.0
51	33.474506328520896	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	4.0
15	7.0
16	31.0
17	53.0
18	125.0
19	241.0
20	407.0
21	672.0
22	978.0
23	1497.0
24	2099.0
25	3046.0
26	4078.0
27	4771.0
28	4830.0
29	5255.0
30	5966.0
31	7007.0
32	8461.0
33	10895.0
34	18980.0
35	27465.0
36	20744.0
37	26780.0
38	37874.0
39	63824.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.934466038369344	25.337117692529926	26.48765138087467	15.240764888226064
2	32.939931756603755	26.016818795824193	26.791779559775435	14.251469887796613
3	29.406735326498584	25.545595802328396	31.330277736567997	13.717391134605023
4	25.659985476805836	28.3510708903655	30.71265157607889	15.27629205674977
5	23.85083274121386	32.0689305151049	29.128764513433953	14.951472230247283
6	22.26694567856892	41.13265298154929	26.17883830063012	10.421563039251664
7	86.82839987194603	3.679209188653169	7.900305299404237	1.5920856399965644
8	86.7065924370076	3.12521960475049	7.741409062160833	2.42677889608108
9	81.27523014577851	4.847701665482427	10.306392547883595	3.5706756408554625
10	47.349517064753144	23.571300294367965	15.9298357942079	13.149346846670987
11	34.49453818585004	25.869244403494935	23.107104652887852	16.529112757767177
12	30.094635007144475	21.055508272754956	28.48927548000718	20.360581240093385
13	24.354850044116155	25.8028749677913	30.13836075301981	19.703914235072734
14	22.090090652841003	26.238570792763387	31.32754487745079	20.34379367694482
15	21.441622225171976	22.296226311967583	36.16119183890186	20.100959623958587
16	24.402089465999328	21.021542738012506	33.91361041922059	20.662757376767573
17	24.38452108596013	21.73559978449454	28.84454716524428	25.03533196430105
18	25.419493874491494	21.67820974303316	30.520180212538357	22.38211616993699
19	28.124243583637202	24.424342747382312	26.134722146309468	21.316691522671018
20	29.192401090020383	24.009338570011945	25.792724348213103	21.005535991754574
21	27.83456051721311	24.708950504017302	26.81832733405689	20.6381616447127
22	26.88391595286989	22.319650818686508	26.494288324445037	24.302144903998563
23	25.6061091113523	25.0517291190043	26.009401035363194	23.332760734280203
24	24.98848295086319	22.683511489720544	30.609974154960923	21.71803140445534
25	25.34882994588939	23.129748342716148	28.24878387769284	23.27263783370162
26	23.285130903951714	25.67482099772782	26.671533758618267	24.368514339702195
27	23.438561422960703	24.262323242576382	27.70962981471215	24.589485519750763
28	22.651497997204675	25.042749724762047	29.548844000593423	22.75690827743986
29	23.90275706444082	23.19260410241194	28.54666552146856	24.35797331167868
30	24.88463430440927	24.044084921645027	28.71454115295422	22.35673962099148
31	26.766403010829933	23.930866472503535	25.547157436109657	23.75557308055688
32	27.79356763045498	23.63532727939971	25.919216684495318	22.65188840564999
33	26.570808379726866	24.062043710129537	25.025181344722846	24.341966565420744
34	23.65758056078269	25.61040360425077	27.277838074193223	23.45417776077332
35	23.76572370013508	25.39567895932725	27.842368686119418	22.996228654418253
36	27.260269694154022	26.600089013125533	23.97888671127734	22.160754581443108
37	25.47415105683566	26.768745461501826	25.4296444940697	22.32745898759282
38	26.17454380773165	26.40215193135058	25.020886851824383	22.402417409093395
39	25.837621319424382	25.741971250322088	24.418877029147897	24.001530401105637
40	27.31336524271693	24.31268593202208	25.56511622459417	22.80883260066682
41	23.342520945413092	23.60409460377447	26.85034082657276	26.20304362423968
42	25.314474002701626	24.627355138946367	25.284412552412334	24.773758305939676
43	24.299021636436038	23.705991208001812	26.899532290682508	25.095454864879635
44	24.034715118957454	24.748772165439483	26.729314208524958	24.487198507078105
45	23.264829664795307	25.172365328606787	25.995346331331838	25.567458675266064
46	24.396233339319593	25.066955048371607	26.394343762444265	24.14246784986453
47	24.066728611473323	24.680841095954587	27.413309804717695	23.839120487854395
48	24.87097000882323	23.252336594545213	27.822457855408327	24.05423554122323
49	24.40443191667122	23.690374870189192	27.774047208189206	24.13114600495038
50	23.686861194181354	25.069687907488813	27.464453311054026	23.778997587275807
51	22.842407726963952	25.875100530174667	26.67973233596989	24.60275940689149
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	296.5
2	452.0
3	1825.5
4	3199.0
5	2229.5
6	1260.0
7	1300.5
8	1341.0
9	1427.5
10	1514.0
11	1526.5
12	1539.0
13	1525.5
14	1512.0
15	1488.5
16	1465.0
17	1421.0
18	1377.0
19	1327.5
20	1278.0
21	1257.5
22	1237.0
23	1260.5
24	1284.0
25	1378.0
26	1615.5
27	1759.0
28	1962.5
29	2166.0
30	2524.5
31	2883.0
32	3207.0
33	3531.0
34	4193.0
35	4855.0
36	5351.0
37	5847.0
38	6214.0
39	6581.0
40	7658.0
41	8735.0
42	10290.0
43	11845.0
44	12567.0
45	13289.0
46	13724.5
47	14160.0
48	15142.0
49	16124.0
50	17101.5
51	18079.0
52	18006.5
53	17934.0
54	16584.0
55	15234.0
56	15141.0
57	15048.0
58	14947.0
59	14846.0
60	14252.0
61	13658.0
62	13216.0
63	12774.0
64	11354.5
65	9935.0
66	8890.5
67	7846.0
68	6916.0
69	5986.0
70	5291.5
71	4597.0
72	3993.0
73	3389.0
74	2970.5
75	2050.0
76	1548.0
77	1251.0
78	954.0
79	727.5
80	501.0
81	364.5
82	228.0
83	164.0
84	100.0
85	72.0
86	44.0
87	40.0
88	36.0
89	21.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	256142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.295212957609664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.942580293386534	24.506937728221807
2	13.420671048596583	11.35260280068838
3	8.529274913557442	10.822424965287293
4	6.100348985193571	10.320622377780001
5	4.476946685760588	9.467670673705442
6	3.243783440805255	8.231790685033562
7	2.2089082282333656	6.539837074186251
8	1.4927371471001647	5.050850842106894
9	0.9085313091125603	3.4583872677814473
>10	1.6553892078413857	8.656862221772348
>50	0.012893982160153803	0.40607877096353423
>100	0.006942913470852048	0.4148883310774126
>500	0.0	0.0
>1k	9.918447815502926E-4	0.7710462613956363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1838	0.7175707224898689	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9040844531548904E-4	0.0	0.0	0.05192432322696004	0.0
2	3.9040844531548904E-4	0.0	0.0	0.14054704031357607	0.0
3	3.9040844531548904E-4	0.0	0.0	0.22721771517361464	0.0
4	3.9040844531548904E-4	0.0	0.0	0.4025111071202692	0.0
5	3.9040844531548904E-4	0.0	0.0	0.6558861881300216	0.0
6	3.9040844531548904E-4	0.0	0.0	1.3066970664709419	0.0
7	3.9040844531548904E-4	0.0	0.0	1.626831991629643	0.0
8	3.9040844531548904E-4	0.0	0.0	2.3990598964636805	0.0
9	3.9040844531548904E-4	0.0	0.0	2.896830664240929	0.0
10	3.9040844531548904E-4	0.0	0.0	3.5320252047692295	0.0
11	3.9040844531548904E-4	0.0	0.0	3.941954072350493	0.0
12	3.9040844531548904E-4	0.0	0.0	4.2577945046107235	0.0
13	3.9040844531548904E-4	0.0	0.0	4.431135854330801	0.0
14	3.9040844531548904E-4	0.0	0.0	4.5435734865816615	0.0
15	3.9040844531548904E-4	0.0	0.0	4.628292119215123	0.0
16	3.9040844531548904E-4	0.0	0.0	4.76532548352086	0.0
17	3.9040844531548904E-4	0.0	0.0	4.912509467404799	0.0
18	3.9040844531548904E-4	0.0	0.0	5.100686338046865	0.0
19	3.9040844531548904E-4	0.0	0.0	5.2060966182820465	0.0
20	3.9040844531548904E-4	0.0	0.0	5.331808137673634	0.0
21	3.9040844531548904E-4	0.0	0.0	5.461423741518376	0.0
22	3.9040844531548904E-4	0.0	0.0	5.626566513886829	0.0
23	3.9040844531548904E-4	0.0	0.0	5.784681934239601	0.0
24	3.9040844531548904E-4	0.0	0.0	5.9178112140921835	0.0
25	3.9040844531548904E-4	0.0	0.0	6.0407898743665625	0.0
26	3.9040844531548904E-4	0.0	0.0	6.150885055945531	0.0
27	3.9040844531548904E-4	0.0	0.0	6.268788406430808	0.0
28	3.9040844531548904E-4	0.0	0.0	6.385130123134823	0.0
29	3.9040844531548904E-4	0.0	0.0	6.517478586096774	0.0
30	3.9040844531548904E-4	0.0	0.0	6.699799330059108	0.0
31	3.9040844531548904E-4	0.0	0.0	6.838784736591422	0.0
32	3.9040844531548904E-4	0.0	0.0	6.986749537365992	0.0
33	3.9040844531548904E-4	0.0	0.0	7.124173310117045	0.0
34	3.9040844531548904E-4	0.0	0.0	7.285411998032341	0.0
35	3.9040844531548904E-4	0.0	0.0	7.474760094010354	0.0
36	3.9040844531548904E-4	0.0	0.0	7.640683683269437	0.0
37	3.9040844531548904E-4	0.0	0.0	7.803484004965996	0.0
38	3.9040844531548904E-4	0.0	0.0	7.97096922800634	0.0
39	3.9040844531548904E-4	0.0	0.0	8.229029210359878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACGG	30	2.158873E-6	45.000004	2
ACAACGA	40	6.7793735E-9	45.0	13
ACCGGCC	25	3.8822895E-5	45.0	21
CTATATG	25	3.8822895E-5	45.0	1
CGTTAGG	50	2.1827873E-11	45.0	2
GCCGATA	25	3.8822895E-5	45.0	9
GCGATAA	25	3.8822895E-5	45.0	9
CACAACG	40	6.7793735E-9	45.0	12
TAGTGCG	25	3.8822895E-5	45.0	1
TATATGG	80	0.0	42.1875	2
CGTTTTT	1125	0.0	42.0	1
GTCAAGC	130	0.0	41.53846	16
GGTATGC	60	3.6379788E-12	41.250004	8
AACACGT	110	0.0	40.909092	41
CGACCCG	50	1.0750227E-9	40.5	32
TAGTAGG	100	0.0	40.5	2
GGGCGAT	575	0.0	40.304348	7
GGTACCT	185	0.0	40.135136	8
ACACGAC	135	0.0	40.000004	26
CGACCAA	135	0.0	40.000004	29
>>END_MODULE