FastQCFastQC Report
Thu 26 May 2016
SRR1546963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546963_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3702014
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529101.4292220396789423No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT38870.10499690168648741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT308650.043.308761
GCGTAAG3400.038.382351
AGGGCGA42900.038.0244756
TACGGGA11150.037.7354284
GGGCGAT84850.037.495587
CACGACC23900.036.90376727
CGTTAGG4550.036.5934072
GACACGA24000.036.562525
ACGGGAT12200.036.5163965
ACACGAC24150.036.33540326
GTTTTTT378150.036.223722
AGGGATT53400.036.109556
ACGACCA24400.036.0553328
TCGATAG2000.036.01
GGCGATT20850.035.9352538
TAAGGGA40650.035.922514
TAGGGAC28400.035.8890845
GCGAGAC24650.035.8722121
GTACGGG11500.035.8043483
TAGGGAT41150.035.759425
Produced by FastQC (version 0.11.5)