##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546963_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3702014
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65069716105882	31.0	31.0	33.0	30.0	34.0
2	32.0236193055996	33.0	31.0	34.0	30.0	34.0
3	32.00391300519123	33.0	31.0	34.0	30.0	34.0
4	35.75493852805527	37.0	35.0	37.0	35.0	37.0
5	35.734034501220144	37.0	35.0	37.0	33.0	37.0
6	35.79705911430913	37.0	35.0	37.0	35.0	37.0
7	36.15336003591558	37.0	35.0	37.0	35.0	37.0
8	36.09253665707369	37.0	35.0	37.0	35.0	37.0
9	37.861053199690765	39.0	38.0	39.0	35.0	39.0
10	37.38025869162029	39.0	37.0	39.0	34.0	39.0
11	37.140683152467815	39.0	37.0	39.0	33.0	39.0
12	36.900141922748	39.0	35.0	39.0	33.0	39.0
13	36.86174552554366	39.0	35.0	39.0	33.0	39.0
14	37.99503486480602	40.0	37.0	41.0	33.0	41.0
15	38.060184267266415	40.0	37.0	41.0	33.0	41.0
16	38.104200848511105	40.0	37.0	41.0	33.0	41.0
17	38.047684044414744	40.0	36.0	41.0	33.0	41.0
18	37.87797642040252	39.0	36.0	41.0	33.0	41.0
19	37.73161284641279	39.0	36.0	41.0	33.0	41.0
20	37.524357552402556	39.0	35.0	41.0	33.0	41.0
21	37.47224051556801	39.0	35.0	41.0	33.0	41.0
22	37.400804805168214	39.0	35.0	41.0	33.0	41.0
23	37.28678416667252	39.0	35.0	41.0	32.0	41.0
24	37.18945822463124	39.0	35.0	41.0	32.0	41.0
25	37.07802023439133	39.0	35.0	41.0	32.0	41.0
26	36.94246699229122	39.0	35.0	41.0	32.0	41.0
27	36.86558586758451	39.0	35.0	41.0	32.0	41.0
28	36.867296287912474	39.0	35.0	41.0	32.0	41.0
29	36.84828177311053	39.0	35.0	41.0	32.0	41.0
30	36.72253535507969	39.0	35.0	41.0	31.0	41.0
31	36.56470640035397	39.0	35.0	41.0	31.0	41.0
32	36.327233770590816	39.0	35.0	41.0	30.0	41.0
33	36.023990995171815	39.0	35.0	41.0	29.0	41.0
34	35.68121460372651	39.0	35.0	41.0	27.0	41.0
35	35.4484588659038	39.0	35.0	41.0	25.0	41.0
36	35.27448329476874	38.0	35.0	41.0	24.0	41.0
37	35.18751928004594	38.0	35.0	41.0	24.0	41.0
38	35.06069885203027	38.0	35.0	41.0	23.0	41.0
39	34.951985054621616	38.0	35.0	41.0	23.0	41.0
40	34.81926945711172	38.0	34.0	41.0	23.0	41.0
41	34.72857341976557	38.0	34.0	40.0	22.0	41.0
42	34.64101459367793	38.0	34.0	40.0	22.0	41.0
43	34.52471114371799	38.0	34.0	40.0	21.0	41.0
44	34.37168443987516	38.0	34.0	40.0	21.0	41.0
45	34.273920628068936	37.0	34.0	40.0	20.0	41.0
46	34.22495403853146	37.0	34.0	40.0	20.0	41.0
47	34.17660657145003	37.0	34.0	40.0	20.0	41.0
48	34.06265076253088	37.0	33.0	40.0	20.0	41.0
49	33.98076938660956	37.0	33.0	40.0	20.0	41.0
50	33.88271141059975	37.0	33.0	40.0	20.0	41.0
51	33.52146696365816	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	5.0
12	4.0
13	9.0
14	42.0
15	100.0
16	264.0
17	705.0
18	1581.0
19	3123.0
20	5493.0
21	8824.0
22	13973.0
23	20848.0
24	33334.0
25	52489.0
26	72995.0
27	81383.0
28	78486.0
29	77292.0
30	81937.0
31	94584.0
32	113330.0
33	144162.0
34	231304.0
35	333517.0
36	281746.0
37	357344.0
38	533332.0
39	1079075.0
40	728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.61783991092416	23.259015227927286	26.00919391444765	18.113950946700903
2	36.20499544302101	24.652175815650615	26.047605438553177	13.09522330277519
3	28.38049775068382	24.55293253888289	34.178314830792104	12.88825487964119
4	24.09272358235274	27.760862060489238	33.60200690759138	14.54440744956664
5	22.44975302632567	30.954069865754153	32.57529550131361	14.020881606606567
6	21.007970256190276	39.42137982190235	29.388084431879513	10.18256549002786
7	83.34660538831025	3.1698691577071294	11.76732448877827	1.7162009652043457
8	83.00465638433566	2.836726171213831	11.42934629636733	2.7292711480831784
9	77.27212809027735	5.120293980519793	14.192950107698135	3.4146278215047268
10	37.56611941499951	29.101591728178228	20.067968408547348	13.264320448274914
11	27.727663914831226	25.55400925010008	30.35426122105427	16.364065614014425
12	24.610009578569937	22.36447512083963	33.320835631631866	19.704679668958573
13	22.31728459157637	25.242070937603156	35.18614462289986	17.254499847920616
14	20.008919469240258	26.783718267948203	34.83979261018462	18.367569652626923
15	19.407922282303634	24.22286895727569	38.331567627783144	18.037641132637532
16	22.142866018334885	22.915580546156768	36.705452761658925	18.236100673849425
17	21.768934423262582	23.77173614146246	32.86762286690434	21.591706568370622
18	22.627494115365312	22.913284498653976	34.269859595344585	20.189361790636124
19	23.795696072462178	25.92802188214307	31.019331639480562	19.256950405914186
20	24.976512784662617	25.539827780229896	30.512688498746897	18.970970936360587
21	24.101367525892663	26.195497910056524	31.494640484882012	18.208494079168798
22	23.323925841447384	23.20915047863136	31.630890644930034	21.836033034991225
23	21.483117027650355	26.215730140404652	30.99596597959921	21.305186852345777
24	20.90786258506856	25.21697648901382	34.39387317281891	19.48128775309872
25	21.90604897766459	25.539206496787966	32.21414073528625	20.340603790261195
26	20.370182284561864	27.26515891079828	31.572408964417747	20.79224984022211
27	20.804540447442932	25.232913759915547	31.950014235494518	22.012531557147003
28	20.17636886300268	26.74020141468941	33.10227892168965	19.98115080061826
29	20.826852626705357	24.720922179116556	32.75835801809501	21.693867176083074
30	21.898593576361407	25.290936230927276	33.20759997125889	19.60287022145243
31	23.03824350745297	26.490796631239107	30.251830490106197	20.21912937120173
32	23.025601740025834	25.445041536849942	31.45444614742138	20.074910575702848
33	22.63297761704845	26.774966275114032	29.438759550882303	21.153296556955215
34	21.394732704954656	27.106731633105657	31.368384884552032	20.13015077738766
35	21.691355029991783	28.132794743617932	29.942053163494247	20.233797062896034
36	23.57884113890439	28.338736698456568	27.93960260550068	20.14281955713836
37	22.61523052046805	28.248245414523016	28.31947691175668	20.817047153252258
38	22.876034504461625	28.916044077629095	28.00648511864083	20.20143629926845
39	23.192970096817568	26.876775722620174	27.37185758886919	22.55839659169306
40	24.111713245817008	26.238258418255576	28.317613061430887	21.33241527449653
41	20.830742401298323	26.102143319825373	28.97849656970503	24.088617709171277
42	22.322633031641693	26.635069451385114	28.492652918114302	22.549644598858894
43	21.696460359145046	25.88758443377037	30.04313327826421	22.372821928820365
44	22.391028234901327	26.617808576628832	28.93641136959504	22.054751818874806
45	21.20975231320033	26.419078912181316	28.866530488539482	23.504638286078876
46	22.877925367110983	26.2550600835113	28.52474355850626	22.342270990871455
47	21.830279410072464	26.16613551434435	30.071145057798272	21.932440017784913
48	21.874795719303062	25.795256311834585	30.336865284680176	21.993082684182177
49	22.38414009239295	25.24825675969891	29.657964556590006	22.709638591318132
50	21.28390114137872	25.838611091152003	30.465254858571576	22.412232908897696
51	21.15518741960457	25.931533484206167	29.504993768256956	23.40828532793231
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2489.0
1	3716.5
2	4944.0
3	43871.0
4	82798.0
5	56238.5
6	29679.0
7	29523.5
8	29368.0
9	30917.5
10	32467.0
11	33404.5
12	34342.0
13	33888.5
14	33435.0
15	32422.0
16	31409.0
17	30510.5
18	29612.0
19	28541.5
20	27471.0
21	26758.0
22	26045.0
23	26773.5
24	27502.0
25	29148.0
26	33728.0
27	36662.0
28	41224.5
29	45787.0
30	52305.0
31	58823.0
32	65107.0
33	71391.0
34	81359.5
35	91328.0
36	99717.5
37	108107.0
38	119833.5
39	131560.0
40	152378.5
41	173197.0
42	192127.5
43	211058.0
44	245856.5
45	280655.0
46	293558.0
47	306461.0
48	298803.0
49	291145.0
50	269495.5
51	247846.0
52	231225.0
53	214604.0
54	202483.0
55	190362.0
56	178936.0
57	167510.0
58	157027.5
59	146545.0
60	135538.0
61	124531.0
62	114454.0
63	104377.0
64	92133.0
65	79889.0
66	69705.5
67	59522.0
68	52106.5
69	44691.0
70	37635.5
71	30580.0
72	26207.5
73	21835.0
74	19703.0
75	14627.5
76	11684.0
77	8641.5
78	5599.0
79	4304.5
80	3010.0
81	2365.5
82	1721.0
83	1271.5
84	822.0
85	627.5
86	433.0
87	338.0
88	243.0
89	153.5
90	64.0
91	48.5
92	33.0
93	20.0
94	7.0
95	5.5
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3702014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.90487633438741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75801163066885	19.884067989659467
2	7.553827627730351	3.9136194109526756
3	2.725341616735361	2.1179891265146695
4	1.6141737427964191	1.6725988475742606
5	1.0879227026311487	1.4091251536516216
6	0.7819346083490364	1.2153551598515666
7	0.6586802223279552	1.1944120792318742
8	0.533570765276468	1.1057667752105083
9	0.45746488189207557	1.0665514073465423
>10	6.605064769526339	41.51182715308032
>50	1.1414757653964756	18.851907550745846
>100	0.0781902567144417	3.1012336255021875
>500	0.0019059848583263446	0.3315484382656848
>1k	0.002329537049027378	1.1726671038398724
>5k	0.0	0.0
>10k+	1.0588804768306264E-4	1.4513301785728439
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52910	1.4292220396789423	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	3887	0.10499690168648741	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7012323562255573E-5	0.0	0.0	0.10302500206644276	0.0
2	2.7012323562255573E-5	0.0	0.0	0.2651259557635384	0.0
3	2.7012323562255573E-5	0.0	0.0	0.4149092899162456	0.0
4	2.7012323562255573E-5	0.0	0.0	0.683519835419315	0.0
5	2.7012323562255573E-5	0.0	0.0	1.1488071087791671	0.0
6	2.7012323562255573E-5	0.0	0.0	2.2261666217361684	0.0
7	2.7012323562255573E-5	0.0	0.0	2.762415269094066	0.0
8	2.7012323562255573E-5	0.0	0.0	3.895879378089872	0.0
9	2.7012323562255573E-5	0.0	0.0	4.5815601993941675	0.0
10	5.4024647124511146E-5	0.0	0.0	5.500681520923476	0.0
11	5.4024647124511146E-5	0.0	0.0	6.214076986202645	0.0
12	5.4024647124511146E-5	0.0	0.0	6.770854999467857	0.0
13	5.4024647124511146E-5	0.0	0.0	7.128930360609117	0.0
14	5.4024647124511146E-5	0.0	0.0	7.350917635643733	0.0
15	5.4024647124511146E-5	0.0	0.0	7.521068261762381	0.0
16	5.4024647124511146E-5	0.0	0.0	7.782547553845015	0.0
17	5.4024647124511146E-5	0.0	0.0	8.056398490119163	0.0
18	5.4024647124511146E-5	0.0	0.0	8.402858552128652	0.0
19	5.4024647124511146E-5	0.0	0.0	8.615580600181415	0.0
20	5.4024647124511146E-5	0.0	0.0	8.84426693145947	0.0
21	5.4024647124511146E-5	0.0	0.0	9.094536109263768	0.0
22	5.4024647124511146E-5	0.0	0.0	9.370494006775772	0.0
23	5.4024647124511146E-5	0.0	0.0	9.672356722583977	0.0
24	5.4024647124511146E-5	0.0	0.0	9.93118880695751	0.0
25	5.4024647124511146E-5	0.0	0.0	10.156768721025907	0.0
26	5.4024647124511146E-5	0.0	0.0	10.366546425810384	0.0
27	5.4024647124511146E-5	0.0	0.0	10.596259225383804	0.0
28	5.4024647124511146E-5	0.0	0.0	10.826782394664093	0.0
29	5.4024647124511146E-5	0.0	0.0	11.07105483663757	0.0
30	5.4024647124511146E-5	0.0	0.0	11.402469034422884	0.0
31	5.4024647124511146E-5	0.0	0.0	11.658518849469505	0.0
32	5.4024647124511146E-5	0.0	0.0	11.949414561911436	0.0
33	5.4024647124511146E-5	0.0	0.0	12.212757704319865	0.0
34	5.4024647124511146E-5	0.0	0.0	12.478667017466709	0.0
35	5.4024647124511146E-5	0.0	0.0	12.765213745815116	0.0
36	8.103697068676671E-5	0.0	0.0	13.029961529048782	0.0
37	8.103697068676671E-5	0.0	0.0	13.302434836821254	0.0
38	8.103697068676671E-5	0.0	0.0	13.606728661750063	0.0
39	8.103697068676671E-5	0.0	0.0	13.990087557745595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	30865	0.0	43.30876	1
GCGTAAG	340	0.0	38.38235	1
AGGGCGA	4290	0.0	38.024475	6
TACGGGA	1115	0.0	37.735428	4
GGGCGAT	8485	0.0	37.49558	7
CACGACC	2390	0.0	36.903767	27
CGTTAGG	455	0.0	36.593407	2
GACACGA	2400	0.0	36.5625	25
ACGGGAT	1220	0.0	36.516396	5
ACACGAC	2415	0.0	36.335403	26
GTTTTTT	37815	0.0	36.22372	2
AGGGATT	5340	0.0	36.10955	6
ACGACCA	2440	0.0	36.05533	28
TCGATAG	200	0.0	36.0	1
GGCGATT	2085	0.0	35.935253	8
TAAGGGA	4065	0.0	35.92251	4
TAGGGAC	2840	0.0	35.889084	5
GCGAGAC	2465	0.0	35.87221	21
GTACGGG	1150	0.0	35.804348	3
TAGGGAT	4115	0.0	35.75942	5
>>END_MODULE