FastQCFastQC Report
Thu 26 May 2016
SRR1546962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546962_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2153738
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221251.027283727175729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT200050.043.481631
TCGATTG1050.040.7142871
TCGTTAG1200.039.3749961
AGGGCGA25100.038.8147436
GGGCGAT52550.038.1493847
AGGGTAC12900.037.56
CGTTAGG3450.037.1739122
TAACGGG6000.037.1253
TATGGGC10700.036.7990654
GTTTTTT241350.036.7961462
GCGATAA2700.036.6666649
TAGGGAT13400.036.6044775
TAGGGTA12750.036.529415
GTACGGG6150.036.2195133
CGTATGG2000.036.0000042
ATTAGCG1750.036.0000041
CGCGATA250.002107516236.0000043
ACGGGTA2000.036.0000045
CGGTAGT5850.035.76923412
TTAACGG1700.035.7352942
Produced by FastQC (version 0.11.5)