FastQCFastQC Report
Thu 26 May 2016
SRR1546961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546961_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2999040
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293810.9796801643192488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT178650.042.9596981
TATAGCG1800.038.7500041
CGTTAGG4400.038.3522722
GGGCGAT76750.038.2573247
TAGGGCG15250.037.9180345
AGGGCGA39050.037.797696
TGGGCGA22100.037.364256
ACGGGAT10250.036.878055
GGCGATT21450.036.39868
GACCGAT21250.036.3176469
TAACGGG8450.036.2130173
TAGGGAC25150.036.0536775
TATGGGC11550.036.0389634
AGGGATT33700.035.786356
TACGGGA9500.035.7631574
CGGGTAC3800.035.5263146
ACGACCA11350.035.4845828
GTTTTTT225350.035.2950972
CGCATCG1150.035.21739221
TAGGGAT29200.035.214045
Produced by FastQC (version 0.11.5)