##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546961_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2999040
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673856300682885	31.0	31.0	33.0	30.0	34.0
2	32.062886790439606	33.0	31.0	34.0	30.0	34.0
3	32.09922241784037	33.0	31.0	34.0	30.0	34.0
4	35.81208486715749	37.0	35.0	37.0	35.0	37.0
5	35.778848564874096	37.0	35.0	37.0	35.0	37.0
6	35.83928690514298	37.0	35.0	37.0	35.0	37.0
7	36.16703111662399	37.0	35.0	37.0	35.0	37.0
8	36.1072489863423	37.0	35.0	37.0	35.0	37.0
9	37.88060245945369	39.0	38.0	39.0	35.0	39.0
10	37.39297275128041	39.0	37.0	39.0	34.0	39.0
11	37.182383362676056	39.0	37.0	39.0	34.0	39.0
12	36.86961260936833	39.0	35.0	39.0	33.0	39.0
13	36.79922608568075	39.0	35.0	39.0	33.0	39.0
14	37.94087141218523	40.0	36.0	41.0	33.0	41.0
15	38.010408664105846	40.0	36.0	41.0	33.0	41.0
16	38.06177110008536	40.0	36.0	41.0	33.0	41.0
17	38.01028329065301	40.0	36.0	41.0	33.0	41.0
18	37.87749079705506	39.0	36.0	41.0	33.0	41.0
19	37.78193521927017	39.0	36.0	41.0	33.0	41.0
20	37.60884816474605	39.0	35.0	41.0	33.0	41.0
21	37.54793333866837	39.0	35.0	41.0	33.0	41.0
22	37.465672681924886	39.0	35.0	41.0	33.0	41.0
23	37.36262103873239	39.0	35.0	41.0	33.0	41.0
24	37.27279762857448	39.0	35.0	41.0	32.0	41.0
25	37.17174562526675	39.0	35.0	41.0	32.0	41.0
26	37.05071356167307	39.0	35.0	41.0	32.0	41.0
27	36.96108388017499	39.0	35.0	41.0	32.0	41.0
28	36.97413905783184	39.0	35.0	41.0	32.0	41.0
29	36.971169107447714	39.0	35.0	41.0	32.0	41.0
30	36.87921868331199	39.0	35.0	41.0	32.0	41.0
31	36.753101992637646	39.0	35.0	41.0	31.0	41.0
32	36.586805444408874	39.0	35.0	41.0	31.0	41.0
33	36.37534344323517	39.0	35.0	41.0	30.0	41.0
34	36.11950624199744	39.0	35.0	41.0	30.0	41.0
35	35.95124573196756	39.0	35.0	41.0	29.0	41.0
36	35.8223691581306	39.0	35.0	41.0	28.0	41.0
37	35.74440554310713	39.0	35.0	41.0	28.0	41.0
38	35.6387450650875	38.0	35.0	41.0	27.0	41.0
39	35.547305804524115	38.0	35.0	41.0	27.0	41.0
40	35.42632775821596	38.0	35.0	41.0	26.0	41.0
41	35.36687640044814	38.0	35.0	41.0	26.0	41.0
42	35.26668934058899	38.0	35.0	41.0	25.0	41.0
43	35.14516578638498	38.0	35.0	40.0	25.0	41.0
44	34.98569275501494	38.0	34.0	40.0	24.0	41.0
45	34.89425116037132	38.0	34.0	40.0	24.0	41.0
46	34.84417780356381	38.0	34.0	40.0	24.0	41.0
47	34.788202558151944	37.0	34.0	40.0	23.0	41.0
48	34.695529902902265	37.0	34.0	40.0	23.0	41.0
49	34.601545161118224	37.0	34.0	40.0	24.0	41.0
50	34.49792333546735	37.0	34.0	40.0	24.0	41.0
51	34.137963815087495	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	5.0
13	9.0
14	25.0
15	54.0
16	199.0
17	474.0
18	1045.0
19	2085.0
20	3603.0
21	6006.0
22	9395.0
23	14346.0
24	22100.0
25	34788.0
26	47651.0
27	53545.0
28	53981.0
29	55898.0
30	62232.0
31	73208.0
32	89709.0
33	115840.0
34	192170.0
35	299028.0
36	221218.0
37	286168.0
38	437429.0
39	916107.0
40	715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22493197823304	23.935792787025182	26.98453505121639	15.854740183525395
2	34.20571249466496	25.12727406103286	27.347451184379	13.319562259923176
3	28.97187099871959	24.930277688860436	32.58749466495946	13.510356647460519
4	24.678697183098592	28.21126093683312	32.321309485702095	14.788732394366196
5	22.74591202518139	31.95462548015365	31.127027315407595	14.172435179257361
6	20.873046041399913	40.95260483354674	27.923969003414427	10.250380121638925
7	86.19698303457106	3.115930431071276	9.119418213828427	1.5676683205292359
8	86.08174615877081	2.6398780943235165	8.782743811352967	2.4956319355527103
9	80.38278915919761	4.972791293213828	11.336427656850192	3.3079918907383696
10	39.72521206786171	27.97418507255655	18.396420187793428	13.904182671788307
11	30.991717349551855	25.352646180110973	27.066861395646608	16.588775074690567
12	26.771933685446008	22.772487195902688	31.01622519206146	19.439353926589842
13	22.824437153222366	27.095503894579597	32.451317755014934	17.6287411971831
14	20.57024914639351	28.892779022620573	32.221710947503205	18.315260883482715
15	19.614976792573625	24.212147887323944	37.898494184805806	18.27438113529663
16	21.891471937686728	22.729073303457106	36.34906503414426	19.030389724711906
17	21.68493918053777	23.50402128681178	31.30711827784891	23.503921254801536
18	22.960313967136152	23.389384603072983	33.227366090482285	20.42293533930858
19	25.60472684592403	25.76331092616304	28.876707212974818	19.755255014938115
20	26.72952011310286	24.90620331839522	28.70605260349979	19.658223965002133
21	24.64832079598805	26.520319835680752	29.717576291079812	19.113783077251387
22	24.410044547588562	23.57501067008109	29.22538545667947	22.789559325650874
23	21.931784837814767	27.211607714468627	28.93172481860862	21.92488262910798
24	21.719416880068287	24.947783290653007	33.299055697823306	20.0337441314554
25	22.226212387964146	25.560279289372602	31.233794814340587	20.979713508322664
26	20.60496025394793	27.413472311139564	29.955585787451984	22.025981647460522
27	21.016258536064875	26.112989489970122	30.511730420401197	22.359021553563807
28	20.49852619504908	26.14880094963722	33.07171628254375	20.280956572769952
29	21.267272193768672	24.60123906316688	32.05755841869398	22.073930324370465
30	21.983568075117372	25.615096830985916	31.849791933418693	20.55154316047802
31	24.569328851899275	25.129774861288944	29.319482234314982	20.9814140524968
32	25.350612195902688	25.092796361502344	29.447256455399064	20.109334987195904
33	23.86000186726419	24.906003254374735	29.716209186939817	21.517785691421253
34	21.755228339735382	26.175309432351686	30.65077491463935	21.418687313273583
35	21.633289319248828	26.108154609475033	30.900388124199747	21.3581679470764
36	24.652888924455826	27.480160317968416	27.712101205719165	20.154849551856593
37	22.90803056978233	27.751747225778917	28.775641538625695	20.56458066581306
38	22.635543373879642	28.869538252240716	27.554984261630388	20.939934112249254
39	23.167080132309007	27.444715642338878	27.070695956039266	22.317508269312846
40	23.592516271873667	26.52902262057192	27.711634389671364	22.166826717883055
41	21.09775128040973	25.391725352112676	29.099611875800257	24.410911491677336
42	23.173015364916775	26.47970683952198	27.54551456466069	22.801763230900555
43	22.731840855740504	25.69492237516005	28.8001493811353	22.77308738796415
44	22.41647327144686	26.981033930857873	28.602986288945797	21.999506508749466
45	21.181711481007255	27.401735221937685	28.17765018139138	23.23890311566368
46	22.942341549295776	26.855293693982073	28.068882042253524	22.13348271446863
47	22.24438486982501	26.106120625266755	29.34442354886897	22.305070956039266
48	23.071983034571062	25.256348698250108	29.440887750746906	22.230780516431924
49	22.575324103713186	24.64998799615877	29.4124453158344	23.36224258429364
50	21.4739716709347	26.392445582586426	29.873159411011525	22.26042333546735
51	21.137930804524114	26.643792680324367	28.531863529662825	23.68641298548869
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1787.0
1	2828.0
2	3869.0
3	26104.0
4	48339.0
5	33186.5
6	18034.0
7	17837.0
8	17640.0
9	18611.0
10	19582.0
11	19718.0
12	19854.0
13	19662.5
14	19471.0
15	18999.5
16	18528.0
17	17994.5
18	17461.0
19	16888.5
20	16316.0
21	16737.0
22	17158.0
23	18312.5
24	19467.0
25	20743.0
26	25120.0
27	28221.0
28	31980.0
29	35739.0
30	40829.0
31	45919.0
32	52601.5
33	59284.0
34	67175.0
35	75066.0
36	83299.5
37	91533.0
38	100781.5
39	110030.0
40	128344.5
41	146659.0
42	169123.5
43	191588.0
44	213945.0
45	236302.0
46	244629.0
47	252956.0
48	241667.5
49	230379.0
50	219537.0
51	208695.0
52	195649.5
53	182604.0
54	170852.0
55	159100.0
56	149188.0
57	139276.0
58	131898.5
59	124521.0
60	115758.0
61	106995.0
62	96610.0
63	86225.0
64	76496.0
65	66767.0
66	57943.0
67	49119.0
68	41937.5
69	34756.0
70	31120.0
71	27484.0
72	22552.5
73	17621.0
74	16070.5
75	11403.0
76	8286.0
77	6502.0
78	4718.0
79	3700.0
80	2682.0
81	2020.5
82	1359.0
83	913.0
84	467.0
85	402.0
86	337.0
87	241.0
88	145.0
89	116.5
90	88.0
91	70.5
92	53.0
93	34.5
94	16.0
95	10.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2999040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.650596401064973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0131103587582	18.214059906856665
2	7.111949263823157	3.3640368332706525
3	2.536238469161387	1.7995065723297257
4	1.4341813574076017	1.3567697779991479
5	0.9867474509572374	1.1668582856184635
6	0.751784671803743	1.066809351200245
7	0.5972472573616818	0.9887677684852882
8	0.5399989192650568	1.0217037197239303
9	0.43988892555232556	0.9363271895582553
>10	6.920635150805178	40.88853809924225
>50	1.5709206927688235	24.216340150357006
>100	0.0938603055802698	3.205084013981132
>500	0.0018618040758270568	0.3087713792947074
>1k	0.0014321569814054276	0.4712523255953557
>5k	0.0	0.0
>10k+	1.4321569814054275E-4	0.9951746264872526
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29381	0.9796801643192488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.66880068288519E-5	0.0	0.0	0.06918880708493384	0.0
2	6.66880068288519E-5	0.0	0.0	0.18119131455399062	0.0
3	6.66880068288519E-5	0.0	0.0	0.2940941101152369	0.0
4	6.66880068288519E-5	0.0	0.0	0.499126387110542	0.0
5	6.66880068288519E-5	0.0	0.0	0.8353673175416133	0.0
6	6.66880068288519E-5	0.0	0.0	1.6359568395219803	0.0
7	6.66880068288519E-5	0.0	0.0	2.0473551536491676	0.0
8	6.66880068288519E-5	0.0	0.0	3.0593456572769955	0.0
9	6.66880068288519E-5	0.0	0.0	3.6722417840375585	0.0
10	6.66880068288519E-5	0.0	0.0	4.4569262163892445	0.0
11	6.66880068288519E-5	0.0	0.0	5.005201664532651	0.0
12	6.66880068288519E-5	0.0	0.0	5.469183472044388	0.0
13	6.66880068288519E-5	0.0	0.0	5.7345350512163895	0.0
14	6.66880068288519E-5	0.0	0.0	5.877247385830132	0.0
15	6.66880068288519E-5	0.0	0.0	5.999153062313273	0.0
16	1.0003201024327785E-4	0.0	0.0	6.19191474605207	0.0
17	1.0003201024327785E-4	0.0	0.0	6.413118864703372	0.0
18	1.0003201024327785E-4	0.0	0.0	6.7142152155356385	0.0
19	1.0003201024327785E-4	0.0	0.0	6.88426963294921	0.0
20	1.0003201024327785E-4	0.0	0.0	7.062793427230047	0.0
21	1.0003201024327785E-4	0.0	0.0	7.274427816901408	0.0
22	1.0003201024327785E-4	0.0	0.0	7.510536705078959	0.0
23	1.0003201024327785E-4	0.0	0.0	7.770653275714896	0.0
24	1.0003201024327785E-4	0.0	0.0	7.983388017498933	0.0
25	1.0003201024327785E-4	0.0	0.0	8.168713988476313	0.0
26	1.0003201024327785E-4	0.0	0.0	8.345337174562527	0.0
27	1.0003201024327785E-4	0.0	0.0	8.519893032437047	0.0
28	1.0003201024327785E-4	0.0	0.0	8.714655356380709	0.0
29	1.0003201024327785E-4	0.0	0.0	8.912685392658984	0.0
30	1.0003201024327785E-4	0.0	0.0	9.189040492957746	0.0
31	1.0003201024327785E-4	0.0	0.0	9.405409731113956	0.0
32	1.0003201024327785E-4	0.0	0.0	9.627647513871105	0.0
33	1.0003201024327785E-4	0.0	0.0	9.851119024754588	0.0
34	1.0003201024327785E-4	0.0	0.0	10.069222151088349	0.0
35	1.0003201024327785E-4	0.0	0.0	10.312533344003414	0.0
36	1.0003201024327785E-4	0.0	0.0	10.53173682244985	0.0
37	1.0003201024327785E-4	0.0	0.0	10.756275341442596	0.0
38	1.0003201024327785E-4	0.0	0.0	11.00282090268886	0.0
39	1.0003201024327785E-4	0.0	0.0	11.293180484421681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17865	0.0	42.959698	1
TATAGCG	180	0.0	38.750004	1
CGTTAGG	440	0.0	38.352272	2
GGGCGAT	7675	0.0	38.257324	7
TAGGGCG	1525	0.0	37.918034	5
AGGGCGA	3905	0.0	37.79769	6
TGGGCGA	2210	0.0	37.36425	6
ACGGGAT	1025	0.0	36.87805	5
GGCGATT	2145	0.0	36.3986	8
GACCGAT	2125	0.0	36.317646	9
TAACGGG	845	0.0	36.213017	3
TAGGGAC	2515	0.0	36.053677	5
TATGGGC	1155	0.0	36.038963	4
AGGGATT	3370	0.0	35.78635	6
TACGGGA	950	0.0	35.763157	4
CGGGTAC	380	0.0	35.526314	6
ACGACCA	1135	0.0	35.48458	28
GTTTTTT	22535	0.0	35.295097	2
CGCATCG	115	0.0	35.217392	21
TAGGGAT	2920	0.0	35.21404	5
>>END_MODULE