FastQCFastQC Report
Thu 26 May 2016
SRR1546960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546960_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2162992
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114020.5271401835975352No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT25920.11983400770784172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.034668E-445.016
TCGATAG1550.042.096771
CGTTTTT72900.041.512351
GCGTAAG1550.039.1935461
ACGGGAT5800.039.1810345
TTACGGG6250.038.523
GGGCGAT43450.038.2681247
GTAGTAG3550.038.0281681
TAGGGTA8600.037.9360435
GGACTAA13650.037.7472538
CGGTCTA3000.037.531
TCCGTAC301.14025475E-437.49999624
AGGGCGA20000.037.356
CACGACC7750.037.1612927
GGTACCT18000.037.08
TAAGGGA26500.036.6792454
GGCGATT11800.036.610178
TAGCATA16850.036.5875430
ATAGGGA26150.036.567884
CATACGA2100.036.4285718
Produced by FastQC (version 0.11.5)