##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546960_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2162992
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59315799596115	31.0	31.0	33.0	30.0	34.0
2	31.98383859024906	31.0	31.0	34.0	30.0	34.0
3	32.09426294688099	33.0	31.0	34.0	30.0	34.0
4	35.77203475556082	37.0	35.0	37.0	35.0	37.0
5	35.7155958043303	37.0	35.0	37.0	33.0	37.0
6	35.767234922736655	37.0	35.0	37.0	33.0	37.0
7	36.08406179958132	37.0	35.0	37.0	35.0	37.0
8	36.00721038265514	37.0	35.0	37.0	35.0	37.0
9	37.764100375775776	39.0	38.0	39.0	35.0	39.0
10	37.33030958967948	39.0	37.0	39.0	34.0	39.0
11	37.10404384297307	39.0	37.0	39.0	33.0	39.0
12	36.61555012686131	39.0	35.0	39.0	33.0	39.0
13	36.44181716807089	39.0	35.0	39.0	32.0	39.0
14	37.47705585596248	40.0	35.0	41.0	32.0	41.0
15	37.61640496127586	40.0	35.0	41.0	33.0	41.0
16	37.71372339795986	40.0	35.0	41.0	33.0	41.0
17	37.66950548129628	40.0	35.0	41.0	33.0	41.0
18	37.58730637931162	39.0	36.0	41.0	33.0	41.0
19	37.52047626620903	39.0	35.0	41.0	33.0	41.0
20	37.37225334166747	39.0	35.0	41.0	32.0	41.0
21	37.31147271927034	39.0	35.0	41.0	32.0	41.0
22	37.248489129871956	39.0	35.0	41.0	32.0	41.0
23	37.136344008669475	39.0	35.0	41.0	32.0	41.0
24	37.032424068142646	39.0	35.0	41.0	32.0	41.0
25	36.892078195388606	39.0	35.0	41.0	31.0	41.0
26	36.752434128281564	38.0	35.0	41.0	31.0	41.0
27	36.66236259773499	38.0	35.0	41.0	31.0	41.0
28	36.66217073387234	38.0	35.0	41.0	31.0	41.0
29	36.66926553588733	38.0	35.0	41.0	31.0	41.0
30	36.56835161664953	38.0	35.0	41.0	31.0	41.0
31	36.4345628647725	38.0	35.0	41.0	31.0	41.0
32	36.32136827135745	38.0	35.0	41.0	30.0	41.0
33	36.16263536804574	38.0	35.0	41.0	30.0	41.0
34	35.97117141441115	38.0	35.0	41.0	30.0	41.0
35	35.840262007441545	38.0	35.0	41.0	29.0	41.0
36	35.683889260801706	38.0	35.0	41.0	29.0	41.0
37	35.56349399350529	38.0	35.0	41.0	28.0	41.0
38	35.434389493812276	38.0	35.0	40.0	27.0	41.0
39	35.30152261312109	38.0	35.0	40.0	27.0	41.0
40	35.105802055671035	38.0	34.0	40.0	26.0	41.0
41	35.02927472685983	37.0	34.0	40.0	26.0	41.0
42	34.930291466635104	37.0	34.0	40.0	25.0	41.0
43	34.79244167338575	37.0	34.0	40.0	25.0	41.0
44	34.65313648871563	37.0	34.0	40.0	24.0	41.0
45	34.56056841634181	36.0	34.0	40.0	24.0	41.0
46	34.511512756404095	36.0	34.0	40.0	24.0	41.0
47	34.45566788966395	36.0	34.0	40.0	24.0	41.0
48	34.3423313632228	36.0	34.0	40.0	23.0	41.0
49	34.24940915176755	36.0	34.0	40.0	24.0	41.0
50	34.12960288341335	35.0	33.0	40.0	24.0	41.0
51	33.73860051262326	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	7.0
13	12.0
14	30.0
15	63.0
16	236.0
17	579.0
18	1236.0
19	2112.0
20	3585.0
21	5567.0
22	8425.0
23	12054.0
24	17580.0
25	24143.0
26	30106.0
27	34726.0
28	38394.0
29	43428.0
30	49659.0
31	58781.0
32	71561.0
33	92849.0
34	157803.0
35	245385.0
36	165812.0
37	217902.0
38	315332.0
39	565205.0
40	417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.24570779734738	25.444893000066575	27.87777301071849	13.43162619186756
2	30.51953035425004	26.716095112695747	29.24916042222995	13.515214110824267
3	29.514441107502943	25.765698624867778	30.969370205715048	13.750490061914237
4	24.9634765177125	30.251938056174037	29.740470607380885	15.04411481873257
5	23.67812733472893	33.1202334544002	28.77333804285915	14.428301168011718
6	22.453111245903823	41.793450923535545	25.027878050404258	10.725559780156376
7	87.61040262747157	3.893819302151834	6.727717901869262	1.768060168507327
8	87.04456604555172	3.6100457144547926	6.315696035861436	3.029692204132054
9	81.1764907128644	6.170295590552346	9.172572066840747	3.480641629742505
10	42.29118739227884	30.689988682343717	13.783314963716926	13.235508961660514
11	36.790427333989214	24.678824517150318	22.23993431321059	16.290813835649878
12	32.22748858987921	20.3293400992699	26.70093093270803	20.742240378142867
13	24.68229193635483	27.70093463128851	28.611386449880538	19.005386982476125
14	21.37996811823622	29.272738872820614	29.72724818214769	19.62004482679548
15	19.66562983127076	23.07391798027917	37.145537292787026	20.114914895663045
16	23.135730506631553	20.49665463395149	34.79305517542367	21.574559683993282
17	23.205402516514162	22.107155273805915	27.169818473669803	27.517623736010123
18	24.02019979731779	21.76249380487769	31.476260661158246	22.741045736646274
19	27.366860348998056	25.120989814109347	25.530191512497503	21.981958324395098
20	27.995988889464225	25.025936295649732	24.865371670352918	22.11270314453313
21	26.15335609193192	25.416506394845655	27.33962955017864	21.090507963043784
22	25.808833319771875	22.14677631724944	25.93587955942509	26.108510803553596
23	23.434344648523897	26.04609725787243	25.625013869676817	24.894544223926857
24	23.187048310858295	23.984138637590892	31.152126313920718	21.676686737630096
25	23.967217631872888	23.944979916707968	28.232513111467817	23.855289339951327
26	21.605673992321748	27.457198177339535	26.365608379503946	24.57151945083477
27	22.885937627138706	25.474527876201115	26.850029958501924	24.789504538158255
28	21.04057712649885	26.252940371485423	29.88887614933389	22.817606352681842
29	22.536606700348404	23.56259292683468	29.148050478226455	24.752749894590455
30	23.440955861140495	24.72759954729375	28.945876822475537	22.885567769090223
31	26.767043058874002	24.84572296152737	25.58067713611516	22.80655684348347
32	28.430479631917272	23.680069089483457	25.634075391864602	22.255375886734672
33	27.087340128858546	24.21382048569759	24.807211492229282	23.891627893214583
34	23.41733117829377	25.96865822897172	26.98627641711111	23.627734175623395
35	24.00572910117097	25.768981115048046	27.232740574167636	22.99254920961335
36	28.4922459260136	25.138188213363712	24.69195447787139	21.677611382751298
37	24.991493264884937	27.167090770562258	26.329038664960386	21.512377299592416
38	26.323028471672572	26.297600730839505	24.952057150465652	22.427313647022274
39	25.109894072654914	26.26898296433829	24.722097908822594	23.899025054184204
40	26.998435500454924	23.60290745411911	25.74896254817401	23.649694497251954
41	22.57923284043584	23.214279109677705	27.30994844178804	26.896539608098415
42	25.44392212268931	23.873689777863255	25.57614637502127	25.106241724426166
43	23.774059266053687	22.92620592216707	28.412680213334124	24.887054598445115
44	23.63291218830213	24.79606951851879	27.083826477397977	24.487191815781102
45	22.49767914074578	25.726909762033333	26.311239246377237	25.46417185084365
46	24.567589709069658	24.851594458047003	26.79570705763128	23.78510877525206
47	23.691211063193947	24.249789180912366	28.04356188095009	24.015437874943597
48	24.324592971217644	23.585015571023842	28.35179233210294	23.738599125655576
49	24.84331888421224	22.447239749384188	28.056645609415103	24.652795756988468
50	23.430830997063325	24.755477597698004	27.63815122755886	24.175540177679807
51	22.448950342858414	25.452382625548314	26.581328086280486	25.51733894531279
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1426.0
1	2495.0
2	3564.0
3	11337.0
4	19110.0
5	13606.0
6	8102.0
7	8583.5
8	9065.0
9	10045.0
10	11025.0
11	11166.5
12	11308.0
13	11291.0
14	11274.0
15	11251.0
16	11228.0
17	10923.0
18	10618.0
19	10478.0
20	10338.0
21	9820.5
22	9303.0
23	9711.5
24	10120.0
25	10672.0
26	12470.5
27	13717.0
28	15402.5
29	17088.0
30	19437.0
31	21786.0
32	24952.0
33	28118.0
34	34423.5
35	40729.0
36	44440.5
37	48152.0
38	53413.0
39	58674.0
40	69796.5
41	80919.0
42	96890.0
43	112861.0
44	123248.0
45	133635.0
46	147982.0
47	162329.0
48	168655.0
49	174981.0
50	168560.5
51	162140.0
52	153436.5
53	144733.0
54	137756.5
55	130780.0
56	124204.5
57	117629.0
58	113431.5
59	109234.0
60	106095.0
61	102956.0
62	97684.0
63	92412.0
64	83012.5
65	73613.0
66	65666.5
67	57720.0
68	51433.0
69	45146.0
70	39637.0
71	34128.0
72	28105.5
73	22083.0
74	18330.0
75	13225.0
76	11873.0
77	8789.0
78	5705.0
79	4956.0
80	4207.0
81	3073.0
82	1939.0
83	1363.5
84	788.0
85	601.0
86	414.0
87	281.0
88	148.0
89	96.0
90	44.0
91	29.0
92	14.0
93	11.0
94	8.0
95	7.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2162992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.675437701512678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67301355691102	17.269474418445995
2	7.061587680075608	3.061260076664966
3	2.282718291965607	1.484367543828118
4	1.2372798109721104	1.0727432584826142
5	0.8400963979980031	0.9104728569035457
6	0.5809169356888688	0.7554977309566634
7	0.48095395223357507	0.7297421200254611
8	0.3919830573174024	0.6797123471145463
9	0.32558712362653847	0.6351519073123566
>10	4.428492739588839	24.187368183038686
>50	2.2124750889896423	34.54883657883243
>100	0.479510033271023	12.915178830114199
>500	0.0032311988171028027	0.5043177621272297
>1k	0.0019387192902616816	0.7134968727956852
>5k	0.0	0.0
>10k+	2.1541325447352018E-4	0.5323795133575292
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11402	0.5271401835975352	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	2592	0.11983400770784172	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.623225606012413E-5	0.0	0.0	0.06486385525235415	0.0
2	4.623225606012413E-5	0.0	0.0	0.18654715320260085	0.0
3	4.623225606012413E-5	0.0	0.0	0.2874259359257917	0.0
4	4.623225606012413E-5	0.0	0.0	0.6100808509693979	0.0
5	4.623225606012413E-5	0.0	0.0	1.0009745759577475	0.0
6	4.623225606012413E-5	0.0	0.0	1.9244638907587268	0.0
7	4.623225606012413E-5	0.0	0.0	2.3079604547774566	0.0
8	4.623225606012413E-5	0.0	0.0	3.364413738007353	0.0
9	4.623225606012413E-5	0.0	0.0	3.8855437283170717	0.0
10	4.623225606012413E-5	0.0	0.0	4.66710001701347	0.0
11	4.623225606012413E-5	0.0	0.0	5.303394557168959	0.0
12	4.623225606012413E-5	0.0	0.0	5.819531463824184	0.0
13	4.623225606012413E-5	0.0	0.0	6.0893429101910685	0.0
14	4.623225606012413E-5	0.0	0.0	6.20908445338679	0.0
15	4.623225606012413E-5	0.0	0.0	6.322214783965914	0.0
16	4.623225606012413E-5	0.0	0.0	6.527624697641046	0.0
17	4.623225606012413E-5	0.0	0.0	6.731508946866192	0.0
18	4.623225606012413E-5	0.0	0.0	7.022818392301035	0.0
19	9.246451212024825E-5	0.0	0.0	7.16623085059954	0.0
20	9.246451212024825E-5	0.0	0.0	7.339047023752284	0.0
21	9.246451212024825E-5	0.0	0.0	7.54237648590471	0.0
22	9.246451212024825E-5	0.0	0.0	7.757495173352467	0.0
23	9.246451212024825E-5	0.0	0.0	7.990644440663673	0.0
24	9.246451212024825E-5	0.0	0.0	8.18334048392227	0.0
25	9.246451212024825E-5	0.0	0.0	8.336369251481281	0.0
26	9.246451212024825E-5	0.0	0.0	8.48102998069341	0.0
27	9.246451212024825E-5	0.0	0.0	8.634659767581203	0.0
28	9.246451212024825E-5	0.0	0.0	8.784174883679643	0.0
29	9.246451212024825E-5	0.0	0.0	8.95403219244454	0.0
30	9.246451212024825E-5	0.0	0.0	9.18500854372092	0.0
31	9.246451212024825E-5	0.0	0.0	9.367163632597808	0.0
32	9.246451212024825E-5	0.0	0.0	9.537760657459668	0.0
33	9.246451212024825E-5	0.0	0.0	9.716217165851747	0.0
34	9.246451212024825E-5	0.0	0.0	9.899019506313477	0.0
35	9.246451212024825E-5	0.0	0.0	10.118160400038466	0.0
36	9.246451212024825E-5	0.0	0.0	10.29546110202904	0.0
37	9.246451212024825E-5	0.0	0.0	10.488896861384601	0.0
38	9.246451212024825E-5	0.0	0.0	10.7197345158928	0.0
39	9.246451212024825E-5	0.0	0.0	11.09199664168892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.034668E-4	45.0	16
TCGATAG	155	0.0	42.09677	1
CGTTTTT	7290	0.0	41.51235	1
GCGTAAG	155	0.0	39.193546	1
ACGGGAT	580	0.0	39.181034	5
TTACGGG	625	0.0	38.52	3
GGGCGAT	4345	0.0	38.268124	7
GTAGTAG	355	0.0	38.028168	1
TAGGGTA	860	0.0	37.936043	5
GGACTAA	1365	0.0	37.747253	8
CGGTCTA	300	0.0	37.5	31
TCCGTAC	30	1.14025475E-4	37.499996	24
AGGGCGA	2000	0.0	37.35	6
CACGACC	775	0.0	37.16129	27
GGTACCT	1800	0.0	37.0	8
TAAGGGA	2650	0.0	36.679245	4
GGCGATT	1180	0.0	36.61017	8
TAGCATA	1685	0.0	36.58754	30
ATAGGGA	2615	0.0	36.56788	4
CATACGA	210	0.0	36.42857	18
>>END_MODULE