FastQCFastQC Report
Thu 26 May 2016
SRR1546959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546959_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1838154
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121550.6612612436172377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88900.041.912261
CGTTAGG2300.041.086962
CATACGA3150.040.71428718
ACGGGTA3750.040.25
TCGTTAG451.929402E-840.01
AGGGCGA26250.039.942866
TCGTAAG850.039.7058831
GGGCGAT51750.038.1739127
TACGGGA3950.038.1645554
ATAGGGT12500.038.164
TAGGGCG9750.038.0769235
TAAGGGT12850.037.120624
TTACGAG850.037.0588231
GTACGGG6450.036.976743
TATAGCG1400.036.9642871
TACGGGT3350.036.94034
GGCCGAT6350.036.8503958
CACGACC22550.036.71840327
ACGGGAG7300.036.678085
TATGGGT11350.036.6740074
Produced by FastQC (version 0.11.5)