##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546959_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1838154
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.653268986167646	31.0	31.0	33.0	30.0	34.0
2	32.050755812625056	33.0	31.0	34.0	30.0	34.0
3	32.15317269390921	33.0	31.0	34.0	30.0	34.0
4	35.85541418183678	37.0	35.0	37.0	35.0	37.0
5	35.819097855783575	37.0	35.0	37.0	35.0	37.0
6	35.87581399599816	37.0	35.0	37.0	35.0	37.0
7	36.12533226269399	37.0	35.0	37.0	35.0	37.0
8	36.05381105173995	37.0	35.0	37.0	35.0	37.0
9	37.853178786978674	39.0	38.0	39.0	35.0	39.0
10	37.328048139600924	39.0	37.0	39.0	34.0	39.0
11	37.18858430795243	39.0	37.0	39.0	34.0	39.0
12	36.879185857115345	39.0	35.0	39.0	33.0	39.0
13	36.80554512842776	39.0	35.0	39.0	33.0	39.0
14	37.90942815455071	40.0	36.0	41.0	33.0	41.0
15	37.991006194257935	40.0	36.0	41.0	33.0	41.0
16	38.040491166681356	40.0	36.0	41.0	33.0	41.0
17	37.977556287449254	40.0	36.0	41.0	33.0	41.0
18	37.87782198879963	40.0	36.0	41.0	33.0	41.0
19	37.80587426298341	40.0	36.0	41.0	33.0	41.0
20	37.663545056616584	40.0	35.0	41.0	33.0	41.0
21	37.61078777947876	40.0	35.0	41.0	33.0	41.0
22	37.531071390101154	39.0	35.0	41.0	33.0	41.0
23	37.4072656589165	39.0	35.0	41.0	33.0	41.0
24	37.303068186887494	39.0	35.0	41.0	32.0	41.0
25	37.16829166653066	39.0	35.0	41.0	32.0	41.0
26	37.03980841648741	39.0	35.0	41.0	32.0	41.0
27	36.95715320914352	39.0	35.0	41.0	32.0	41.0
28	36.98256402891162	39.0	35.0	41.0	32.0	41.0
29	36.96380444728788	39.0	35.0	41.0	32.0	41.0
30	36.86201536976771	39.0	35.0	41.0	31.0	41.0
31	36.73234451520384	39.0	35.0	41.0	31.0	41.0
32	36.6086709818655	39.0	35.0	41.0	31.0	41.0
33	36.43230110208394	39.0	35.0	41.0	30.0	41.0
34	36.220798692601385	39.0	35.0	41.0	30.0	41.0
35	36.0604465131866	39.0	35.0	41.0	29.0	41.0
36	35.93063802053582	39.0	35.0	41.0	29.0	41.0
37	35.8409338934605	39.0	35.0	41.0	29.0	41.0
38	35.70087707558779	38.0	35.0	41.0	28.0	41.0
39	35.594687387455025	38.0	35.0	41.0	27.0	41.0
40	35.448244815178704	38.0	35.0	41.0	27.0	41.0
41	35.35431362116558	38.0	35.0	41.0	26.0	41.0
42	35.25582676968306	38.0	35.0	41.0	26.0	41.0
43	35.14407334749972	38.0	34.0	40.0	26.0	41.0
44	34.92499105080423	38.0	34.0	40.0	24.0	41.0
45	34.84575884283907	37.0	34.0	40.0	24.0	41.0
46	34.80966556665002	37.0	34.0	40.0	24.0	41.0
47	34.75448466232971	37.0	34.0	40.0	24.0	41.0
48	34.652578075612816	37.0	34.0	40.0	24.0	41.0
49	34.558211118328494	36.0	34.0	40.0	24.0	41.0
50	34.454542437684765	36.0	34.0	40.0	24.0	41.0
51	34.09212013792098	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	8.0
14	8.0
15	49.0
16	128.0
17	327.0
18	684.0
19	1318.0
20	2269.0
21	3848.0
22	5852.0
23	8865.0
24	13369.0
25	19857.0
26	26767.0
27	30460.0
28	32126.0
29	34142.0
30	38096.0
31	45350.0
32	55113.0
33	72280.0
34	121519.0
35	180425.0
36	141362.0
37	186419.0
38	278043.0
39	539117.0
40	348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.8435441208952	24.360526919942508	26.300516714051163	14.495412245111128
2	33.59930669573931	25.225960392872416	27.419084581596536	13.755648329791736
3	30.16308753238303	25.2430427483225	30.914330355345637	13.67953936394883
4	25.612707096358626	28.766686577947226	30.298767132677675	15.321839193016473
5	23.91355675313385	32.060262633054684	29.04049388680165	14.985686727009814
6	22.315159665621053	41.38608625827869	25.934007705556773	10.364746370543491
7	87.7813828438749	3.265123596825946	7.504539880771688	1.4489536785274792
8	87.51557268868659	2.8558542973004437	7.4085740367782025	2.2199989772347695
9	82.10345814333293	4.402895513651195	10.327807136942823	3.165839206073049
10	39.03176774089657	23.24157823555589	24.02845463437775	13.698199389169789
11	33.97457449158232	26.958677020532555	22.83954445601402	16.227204031871107
12	28.56273195825812	23.509510084573982	27.92475494436266	20.003003012805237
13	23.82537045318292	26.997683545557116	30.355400037211243	18.821545964048713
14	22.058271504999034	27.310660586653785	31.39666208598409	19.234405822363087
15	22.4188506512512	23.452985984852194	34.99429318762193	19.133870176274677
16	24.983924089058913	22.152605276815763	33.09336432094373	19.770106313181593
17	24.307865391039055	22.61698421350986	29.451232051286237	23.623918344164853
18	25.510212963658102	22.40274753910717	30.58720868871705	21.499830808517675
19	26.986694259566935	25.44552850305252	26.797809106309916	20.76996813107063
20	28.618059205050283	24.94236065095743	26.384242016718947	20.05533812727334
21	27.373549767864937	25.421428237242367	27.605412821776632	19.59960917311607
22	26.214941729583046	22.886221720269358	27.2051743216292	23.693662228518395
23	24.45730880002437	26.207923819222984	27.305057138846912	22.029710241905736
24	23.51407988666891	25.047085282299523	31.07057406506745	20.368260765964113
25	24.03552694714371	25.021951370777423	29.341502398602078	21.60101928347679
26	22.621227601169434	26.965477321269056	27.653232536555695	22.760062541005812
27	22.909614754802917	25.371323621415833	28.436572778994577	23.282488844786673
28	21.876893883755116	26.842092664706	29.914522939862493	21.36649051167639
29	22.83949005360813	24.65299425401789	28.99495907307005	23.512556619303933
30	24.14901036583442	25.745829783576347	29.363589775394228	20.741570075195007
31	25.36142238354349	25.957509544902113	27.356358607603067	21.324709463951333
32	26.24905203807733	25.40200657833892	27.039410190876286	21.309531192707468
33	24.60490252720936	26.211351170794178	26.460622994591315	22.723123307405146
34	22.87893179787983	27.216054802807598	28.536455596212285	21.368557803100284
35	22.84405985570306	28.012832439501807	27.892494317668703	21.25061338712643
36	25.95223251153059	27.460920031727483	25.526261673396245	21.060585783345683
37	24.176102763968636	28.209714746424947	26.178872934476654	21.435309555129766
38	24.814297387487663	27.011012135000655	26.6422182254588	21.532472252052877
39	24.82773478174299	26.93294468254564	25.189946000171908	23.04937453553946
40	25.859258799861166	25.466963050973966	26.41508818085971	22.25868996830516
41	22.294813165817445	25.893042693920098	26.96678297901046	24.845361161251994
42	24.263799442266535	26.1860540520544	26.16489151616241	23.385254989516657
43	23.31072369344462	25.275412179828237	27.669770867946863	23.74409325878028
44	23.70987414547421	26.455726778061035	27.25157957385507	22.582819502609684
45	22.58553962290428	26.564858004280385	26.437012350434188	24.41259002238115
46	24.246608282004665	25.88042133575315	26.866628149763294	23.00634223247889
47	23.020106041169562	26.158526434673046	28.46883340568853	22.352534118468856
48	24.075675922692007	25.274596143739856	28.284572456932334	22.3651554766358
49	23.921064285146944	25.18874914724229	27.947603954837298	22.942582612773467
50	22.82991523017114	26.40964794027051	27.783145481825787	22.97729134773256
51	22.86968338887819	26.3260858448204	26.82267100580256	23.98155976049885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	868.0
1	1706.5
2	2545.0
3	12087.5
4	21630.0
5	15405.5
6	9181.0
7	9270.0
8	9359.0
9	9667.0
10	9975.0
11	10213.0
12	10451.0
13	10398.0
14	10345.0
15	10074.5
16	9804.0
17	9589.5
18	9375.0
19	9134.0
20	8893.0
21	8905.5
22	8918.0
23	8897.5
24	8877.0
25	9992.5
26	12534.5
27	13961.0
28	16390.5
29	18820.0
30	21304.5
31	23789.0
32	28427.0
33	33065.0
34	37947.5
35	42830.0
36	47045.5
37	51261.0
38	56768.0
39	62275.0
40	72058.5
41	81842.0
42	91288.0
43	100734.0
44	110902.5
45	121071.0
46	127399.0
47	133727.0
48	134158.5
49	134590.0
50	130479.5
51	126369.0
52	122222.5
53	118076.0
54	112880.5
55	107685.0
56	103973.0
57	100261.0
58	93597.5
59	86934.0
60	84395.5
61	81857.0
62	74322.5
63	66788.0
64	60396.0
65	54004.0
66	47288.5
67	40573.0
68	36015.5
69	31458.0
70	27138.5
71	22819.0
72	20699.5
73	18580.0
74	15949.0
75	11225.0
76	9132.0
77	7285.0
78	5438.0
79	4127.0
80	2816.0
81	2169.5
82	1523.0
83	1120.0
84	717.0
85	502.0
86	287.0
87	212.0
88	137.0
89	97.0
90	57.0
91	38.5
92	20.0
93	11.5
94	3.0
95	5.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1838154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.59558963064333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6853822362655	18.36931012890925
2	6.230621569554161	3.06491622537178
3	2.6040357221570942	1.921433820171355
4	1.558774703273716	1.533559317133926
5	1.1816693215193363	1.4531926855605164
6	0.922699192835331	1.361659841970218
7	0.7912735278139401	1.3623287282992178
8	0.7174747530215013	1.4117371676531216
9	0.642639459220878	1.4225486777509737
>10	10.164912741984638	58.51650972772351
>50	0.4575001336858137	6.799498430983128
>100	0.04032810309089994	1.6138097507500524
>500	0.0020164016828397016	0.35757698179919695
>1k	4.4808926285326697E-4	0.14211604809063474
>5k	0.0	0.0
>10k+	2.2404463142663348E-4	0.6698024678331389
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12155	0.6612612436172377	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.4402405891998165E-5	0.0	0.0	0.05549045400983813	0.0
2	5.4402405891998165E-5	0.0	0.0	0.13121860301149957	0.0
3	5.4402405891998165E-5	0.0	0.0	0.21184296854344087	0.0
4	5.4402405891998165E-5	0.0	0.0	0.34529207019651237	0.0
5	5.4402405891998165E-5	0.0	0.0	0.5571350387399532	0.0
6	5.4402405891998165E-5	0.0	0.0	1.1663875823244407	0.0
7	5.4402405891998165E-5	0.0	0.0	1.4648935834538346	0.0
8	5.4402405891998165E-5	0.0	0.0	2.2293017886423008	0.0
9	5.4402405891998165E-5	0.0	0.0	2.6511924463347465	0.0
10	5.4402405891998165E-5	0.0	0.0	3.2606081971369103	0.0
11	5.4402405891998165E-5	0.0	0.0	3.7329298850912385	0.0
12	5.4402405891998165E-5	0.0	0.0	4.103464671621638	0.0
13	5.4402405891998165E-5	0.0	0.0	4.345446573029245	0.0
14	5.4402405891998165E-5	0.0	0.0	4.487654462030929	0.0
15	5.4402405891998165E-5	0.0	0.0	4.608482205517057	0.0
16	5.4402405891998165E-5	0.0	0.0	4.79453843366769	0.0
17	5.4402405891998165E-5	0.0	0.0	4.972651910558093	0.0
18	5.4402405891998165E-5	0.0	0.0	5.201087612898593	0.0
19	5.4402405891998165E-5	0.0	0.0	5.334536714551664	0.0
20	5.4402405891998165E-5	0.0	0.0	5.468693047481332	0.0
21	5.4402405891998165E-5	0.0	0.0	5.638102139429014	0.0
22	5.4402405891998165E-5	0.0	0.0	5.808272865059184	0.0
23	5.4402405891998165E-5	0.0	0.0	5.998300468839934	0.0
24	5.4402405891998165E-5	0.0	0.0	6.150899217366989	0.0
25	5.4402405891998165E-5	0.0	0.0	6.302464320182096	0.0
26	5.4402405891998165E-5	0.0	0.0	6.440864040771339	0.0
27	5.4402405891998165E-5	0.0	0.0	6.588838584797574	0.0
28	1.0880481178399633E-4	0.0	0.0	6.72838075591055	0.0
29	1.0880481178399633E-4	0.0	0.0	6.8871269763034	0.0
30	1.0880481178399633E-4	0.0	0.0	7.12127493126256	0.0
31	1.0880481178399633E-4	0.0	0.0	7.301564504388642	0.0
32	1.0880481178399633E-4	0.0	0.0	7.508511256401803	0.0
33	1.0880481178399633E-4	0.0	0.0	7.6862439164509615	0.0
34	1.0880481178399633E-4	0.0	0.0	7.867621537694883	0.0
35	1.0880481178399633E-4	0.0	0.0	8.066462331230136	0.0
36	1.0880481178399633E-4	0.0	0.0	8.23891795790777	0.0
37	1.0880481178399633E-4	0.0	0.0	8.421383627269533	0.0
38	1.0880481178399633E-4	0.0	0.0	8.648568074274516	0.0
39	1.0880481178399633E-4	0.0	0.0	9.002346919790181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8890	0.0	41.91226	1
CGTTAGG	230	0.0	41.08696	2
CATACGA	315	0.0	40.714287	18
ACGGGTA	375	0.0	40.2	5
TCGTTAG	45	1.929402E-8	40.0	1
AGGGCGA	2625	0.0	39.94286	6
TCGTAAG	85	0.0	39.705883	1
GGGCGAT	5175	0.0	38.173912	7
TACGGGA	395	0.0	38.164555	4
ATAGGGT	1250	0.0	38.16	4
TAGGGCG	975	0.0	38.076923	5
TAAGGGT	1285	0.0	37.12062	4
TTACGAG	85	0.0	37.058823	1
GTACGGG	645	0.0	36.97674	3
TATAGCG	140	0.0	36.964287	1
TACGGGT	335	0.0	36.9403	4
GGCCGAT	635	0.0	36.850395	8
CACGACC	2255	0.0	36.718403	27
ACGGGAG	730	0.0	36.67808	5
TATGGGT	1135	0.0	36.674007	4
>>END_MODULE