FastQCFastQC Report
Thu 26 May 2016
SRR1546958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546958_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1593863
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108970.6836848587362904No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61650.38679610480950993No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG33890.21262806150842326No Hit
GAATCTGACTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC22900.14367608759347572No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA207.0341077E-445.00000429
CGTTTTT90600.043.4105951
TTAGCGA2300.043.0434762
CGTAAGA1800.042.52
TATGCGA1650.042.2727282
CGACGGT2950.041.1864428
GTTAGCG950.040.2631571
TCACGAC3000.039.75000425
CGGTCTA3000.039.75000431
TAAGCGA1700.039.7058832
AGTGCGA2100.039.6428572
GAGCGAT9600.039.609377
AGCGATA1600.039.3750048
TATAGCG800.039.3750041
TCGAGTA403.4593359E-739.3750045
ATATGCG750.039.0000041
GCGATGT1500.039.0000049
AGGGGAA113250.038.9999968
GTTTTTA67100.038.9307022
GAGGGGA112750.038.7937937
Produced by FastQC (version 0.11.5)