##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546958_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1593863
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702811973174608	31.0	31.0	34.0	30.0	34.0
2	32.057946636567884	33.0	31.0	34.0	30.0	34.0
3	32.04966236119416	33.0	31.0	34.0	30.0	34.0
4	35.813215439470014	37.0	35.0	37.0	35.0	37.0
5	35.79228766838806	37.0	35.0	37.0	35.0	37.0
6	35.84066698329781	37.0	35.0	37.0	35.0	37.0
7	36.0923216110795	37.0	35.0	37.0	35.0	37.0
8	13.286463140182061	0.0	0.0	36.0	0.0	37.0
9	24.96813527887905	18.0	17.0	39.0	17.0	39.0
10	32.66187181708842	32.0	32.0	38.0	27.0	39.0
11	35.4175766675053	37.0	34.0	37.0	32.0	39.0
12	36.601216039270625	37.0	35.0	39.0	33.0	39.0
13	36.86606439825756	39.0	35.0	39.0	33.0	39.0
14	38.07103245385582	40.0	37.0	41.0	33.0	41.0
15	38.36327589008591	40.0	37.0	41.0	34.0	41.0
16	38.457691156642696	40.0	38.0	41.0	34.0	41.0
17	38.41577161901619	40.0	37.0	41.0	34.0	41.0
18	38.3489666301307	40.0	37.0	41.0	34.0	41.0
19	38.33635952399924	40.0	37.0	41.0	34.0	41.0
20	38.2807035485484	40.0	37.0	41.0	34.0	41.0
21	38.233595986606126	40.0	37.0	41.0	34.0	41.0
22	38.195380656932244	40.0	37.0	41.0	34.0	41.0
23	38.067492626405155	40.0	37.0	41.0	34.0	41.0
24	37.95002644518381	40.0	36.0	41.0	34.0	41.0
25	37.87394901569332	40.0	36.0	41.0	33.0	41.0
26	37.799829094470475	40.0	36.0	41.0	33.0	41.0
27	37.723289266392406	40.0	36.0	41.0	33.0	41.0
28	37.67505990163521	40.0	36.0	41.0	33.0	41.0
29	37.677116540129234	40.0	36.0	41.0	33.0	41.0
30	37.56013283450334	40.0	36.0	41.0	33.0	41.0
31	37.481191294358425	40.0	36.0	41.0	33.0	41.0
32	37.30507703610662	40.0	35.0	41.0	33.0	41.0
33	37.12000153087185	40.0	35.0	41.0	32.0	41.0
34	36.91205831366937	40.0	35.0	41.0	31.0	41.0
35	36.72719612664326	40.0	35.0	41.0	31.0	41.0
36	36.61984687517057	40.0	35.0	41.0	30.0	41.0
37	36.56390793939002	40.0	35.0	41.0	30.0	41.0
38	36.47687348285267	40.0	35.0	41.0	30.0	41.0
39	36.40746726663458	40.0	35.0	41.0	30.0	41.0
40	36.30469494555053	40.0	35.0	41.0	30.0	41.0
41	36.24937651479456	40.0	35.0	41.0	29.0	41.0
42	36.22216652246774	39.0	35.0	41.0	29.0	41.0
43	36.11975872455788	39.0	35.0	41.0	29.0	41.0
44	35.96893020290953	39.0	35.0	41.0	28.0	41.0
45	35.895782761755555	39.0	35.0	40.0	28.0	41.0
46	35.86581155343966	39.0	35.0	40.0	28.0	41.0
47	35.805798239873816	39.0	35.0	40.0	28.0	41.0
48	35.74766149913763	39.0	35.0	40.0	27.0	41.0
49	35.66871305752125	39.0	35.0	40.0	27.0	41.0
50	35.55351934262857	39.0	35.0	40.0	27.0	41.0
51	35.194568165519875	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	6.0
15	20.0
16	56.0
17	212.0
18	468.0
19	881.0
20	1736.0
21	3219.0
22	5244.0
23	7984.0
24	11721.0
25	17240.0
26	21797.0
27	23227.0
28	23571.0
29	25395.0
30	30129.0
31	38680.0
32	50740.0
33	69650.0
34	103720.0
35	156870.0
36	163338.0
37	283304.0
38	384545.0
39	170077.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.79649882078949	23.854998829886885	29.174966731770546	16.17353561755308
2	32.53316000183203	26.02532338099322	29.12947976080755	12.312036856367204
3	27.54665865259436	25.274192323932482	34.39304381869709	12.786105204776069
4	25.096385322954358	26.94585419198513	33.732447519015125	14.22531296604539
5	23.70348016109289	30.800953406911386	31.431936119980197	14.063630312015524
6	21.152382607539042	39.65485113839772	28.872305838080187	10.320460415983055
7	85.38048753249181	3.4053742385637915	9.575226979985105	1.6389112489592894
8	32.24662345508993	63.60170227930506	3.208305858157194	0.9433684074478169
9	81.99424919205728	4.824316770010973	9.543166508037391	3.6382675298943514
10	43.82202234445495	31.96780400824914	14.724101130398285	9.486072516897625
11	32.33991880105128	22.881075726081853	29.528008367093033	15.250997105773834
12	30.83426869185118	21.603299656243983	30.21526944285676	17.34716220904808
13	22.729180613390234	30.127746236658986	30.38824541381537	16.754827736135415
14	16.412953936442467	34.09609232412071	31.927148067305662	17.56380567213117
15	15.407911470433783	25.983977292904097	41.775359613718365	16.832751622943753
16	19.222229263117345	23.44367113108216	39.34729647403824	17.986803131762265
17	19.77980541614932	22.476461276784768	31.147658236623847	26.596075070442065
18	21.024893607543437	25.39829332884947	34.366316301965725	19.210496761641373
19	24.65851832936708	26.20896526238453	29.11724533413474	20.015271074113645
20	26.24899379683197	26.034859959733055	28.968549994572935	18.747596248862042
21	22.460399670486108	26.62964131798028	32.68449044867721	18.225468562856406
22	21.68454879748134	22.407070118322594	32.376810303018516	23.531570781177553
23	19.0896582705038	27.3486491624437	31.845585222820276	21.71610734423222
24	19.421305344311275	25.8066094764732	36.329408487429596	18.44267669178593
25	19.489127986533347	27.328760376519185	33.15548450525547	20.026627131691995
26	18.219696423092827	31.416815623425602	29.573997263252867	20.78949069022871
27	18.61038244817779	29.640878795730874	32.54708842604415	19.201650330047187
28	17.063511732187774	27.83783800740716	36.65729112226082	18.441359138144247
29	18.633345525932906	25.535569870183323	35.72992158046206	20.101163023421712
30	19.81801447175824	27.303789597976742	34.25294394813105	18.62525198213397
31	25.305813611332965	25.33134905572185	29.711524767185132	19.651312565760044
32	25.284168087219545	26.336830706277762	30.100955979278016	18.278045227224673
33	23.757625341701264	25.802970518796158	30.391445186945177	20.047958952557405
34	20.061887376769523	26.948050114721276	31.270567169198355	21.719495339310843
35	19.75709330099262	26.02268827370985	32.11957363964155	22.10064478565598
36	24.80846848192097	25.62880247549507	30.53769364117242	19.02503540141154
37	20.288757565738084	28.94815928345159	30.998962897062043	19.76412025374828
38	20.198724733556148	29.578075405477133	28.468444276578353	21.75475558438837
39	20.577301813267514	27.59465524954152	29.808145367575506	22.019897569615456
40	21.82904051351967	25.847328158066286	30.132012600832063	22.191618727581982
41	18.934312422083956	25.567379379532618	30.645984002389167	24.85232419599426
42	21.250948168067143	26.682217982348543	28.87676042420208	23.19007342538223
43	21.42254384473446	26.5905538932769	29.137761526555295	22.849140735433345
44	20.156563017022165	28.75485534202124	29.996931982234358	21.091649658722236
45	19.33466050720796	31.028137299127966	27.77233676922044	21.86486542444363
46	22.109365735950956	28.886736187489138	29.24385596503589	19.760042111524015
47	20.600202150373022	27.518362619622895	30.144623471402497	21.736811758601586
48	21.21468407259595	26.514888669854308	30.756846730239673	21.513580527310065
49	21.019811614925498	25.05867819254227	31.84859677400128	22.072913418530955
50	19.391189832501286	28.475471229334016	30.72309226075265	21.410246677412047
51	19.353545442738803	29.62262126669607	29.210477939446488	21.813355351118634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1336.0
1	2062.5
2	2789.0
3	15209.5
4	27630.0
5	18938.5
6	10247.0
7	10167.0
8	10087.0
9	10266.5
10	10446.0
11	10366.5
12	10287.0
13	9783.5
14	9280.0
15	8623.0
16	7966.0
17	7840.5
18	7715.0
19	7917.0
20	8119.0
21	8875.0
22	9631.0
23	10660.0
24	11689.0
25	14430.5
26	19905.0
27	22638.0
28	25547.5
29	28457.0
30	33013.5
31	37570.0
32	41952.0
33	46334.0
34	51324.5
35	56315.0
36	61471.0
37	66627.0
38	76866.0
39	87105.0
40	101686.0
41	116267.0
42	124066.5
43	131866.0
44	134514.0
45	137162.0
46	141345.0
47	145528.0
48	134206.5
49	122885.0
50	112278.0
51	101671.0
52	92503.0
53	83335.0
54	76032.5
55	68730.0
56	63226.5
57	57723.0
58	51673.0
59	45623.0
60	39395.5
61	33168.0
62	27986.5
63	22805.0
64	18594.0
65	14383.0
66	11584.5
67	8786.0
68	7217.5
69	5649.0
70	4911.0
71	4173.0
72	3315.5
73	2458.0
74	1798.0
75	862.0
76	586.0
77	401.5
78	217.0
79	173.0
80	129.0
81	105.5
82	82.0
83	52.0
84	22.0
85	14.5
86	7.0
87	13.0
88	19.0
89	14.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1593863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.136159799563803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36385790264909	19.435856734556918
2	6.433539506622584	3.3629603324378943
3	2.7016605557841165	2.1183309603045606
4	1.7167556392076362	1.7947759889253228
5	1.323949278245792	1.7301474951374587
6	1.0408640183425615	1.6322512987810376
7	0.8710213724252557	1.59356076449793
8	0.8008622742700202	1.6745171502210672
9	0.7594427374072876	1.7864025069145535
>10	9.480379555193887	53.529233283261746
>50	0.46511557132865944	7.452096551172893
>100	0.03914746195134337	1.8579010529239683
>500	0.0017020632859214516	0.295383042917963
>1k	0.0012157594899438941	0.652283036254297
>5k	2.4315189798877879E-4	0.39178925550543076
>10k+	2.4315189798877879E-4	0.6925105461869714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10897	0.6836848587362904	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6165	0.38679610480950993	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	3389	0.21262806150842326	No Hit
GAATCTGACTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	2290	0.14367608759347572	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012548129920827574	0.0
2	0.0	0.0	0.0	0.062050502458492356	0.0
3	0.0	0.0	0.0	0.09975763287057922	0.0
4	0.0	0.0	0.0	0.17354063680504536	0.0
5	0.0	0.0	0.0	0.3913134315810079	0.0
6	0.0	0.0	0.0	0.5427066190757925	0.0
7	0.0	0.0	0.0	0.6629804443669249	0.0
8	0.0	0.0	0.0	0.8437362558764461	0.0
9	0.0	0.0	0.0	1.0551722450423908	0.0
10	0.0	0.0	0.0	1.595431978783622	0.0
11	0.0	0.0	0.0	1.9566298985546438	0.0
12	0.0	0.0	0.0	2.3555349487377524	0.0
13	0.0	0.0	0.0	2.485031649520693	0.0
14	0.0	0.0	0.0	2.542627565857291	0.0
15	0.0	0.0	0.0	2.634856320775374	0.0
16	0.0	0.0	0.0	2.782798772541931	0.0
17	0.0	0.0	0.0	2.951445638677854	0.0
18	0.0	0.0	0.0	3.164952069280735	0.0
19	0.0	0.0	0.0	3.3019149073665677	0.0
20	0.0	0.0	0.0	3.4328546430904034	0.0
21	0.0	0.0	0.0	3.5888278980062904	0.0
22	0.0	0.0	0.0	3.7609882405200445	0.0
23	0.0	0.0	0.0	3.94726522919473	0.0
24	0.0	0.0	0.0	4.08265955104046	0.0
25	0.0	0.0	0.0	4.200361009697822	0.0
26	0.0	0.0	0.0	4.3138588448317075	0.0
27	0.0	0.0	0.0	4.440594957032066	0.0
28	0.0	0.0	0.0	4.565386109094696	0.0
29	0.0	0.0	0.0	4.715022558400565	0.0
30	0.0	0.0	0.0	4.887559344811944	0.0
31	0.0	0.0	0.0	5.038889791657125	0.0
32	6.274064960413787E-5	0.0	0.0	5.1821266947033715	0.0
33	6.274064960413787E-5	0.0	0.0	5.326618410741701	0.0
34	6.274064960413787E-5	0.0	0.0	5.4735570121145924	0.0
35	6.274064960413787E-5	0.0	0.0	5.6483524619117205	0.0
36	6.274064960413787E-5	0.0	0.0	5.8015651282450245	0.0
37	6.274064960413787E-5	0.0	0.0	5.95766386446012	0.0
38	6.274064960413787E-5	0.0	0.0	6.137855010123204	0.0
39	6.274064960413787E-5	0.0	0.0	6.363784089347704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGATA	20	7.0341077E-4	45.000004	29
CGTTTTT	9060	0.0	43.410595	1
TTAGCGA	230	0.0	43.043476	2
CGTAAGA	180	0.0	42.5	2
TATGCGA	165	0.0	42.272728	2
CGACGGT	295	0.0	41.18644	28
GTTAGCG	95	0.0	40.263157	1
TCACGAC	300	0.0	39.750004	25
CGGTCTA	300	0.0	39.750004	31
TAAGCGA	170	0.0	39.705883	2
AGTGCGA	210	0.0	39.642857	2
GAGCGAT	960	0.0	39.60937	7
AGCGATA	160	0.0	39.375004	8
TATAGCG	80	0.0	39.375004	1
TCGAGTA	40	3.4593359E-7	39.375004	5
ATATGCG	75	0.0	39.000004	1
GCGATGT	150	0.0	39.000004	9
AGGGGAA	11325	0.0	38.999996	8
GTTTTTA	6710	0.0	38.930702	2
GAGGGGA	11275	0.0	38.793793	7
>>END_MODULE