FastQCFastQC Report
Thu 26 May 2016
SRR1546957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546957_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1043337
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.5500619646384629No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31680.3036411054146455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT351.2117926E-745.0000044
CGAGGTA551.8189894E-1245.0000046
TAGACGT253.890557E-545.037
GTTACGA502.1827873E-1145.02
CTAACCG253.890557E-545.036
GATTACG253.890557E-545.01
ACGCAGA650.044.9999962
CGTTTTT49650.043.2779431
CGACGGT2200.042.95454828
ACGACGG2050.042.8048827
ACGGGTA650.041.538465
CGGTCTA2150.040.81395331
CTATGCG451.9279469E-840.01
TCGTTGA451.9279469E-840.02
ACGGTCT2200.039.88636830
GTTTTTA37700.039.6286472
ATAGAGT3300.039.5454524
GAGGGGA81250.039.4061557
AGGGGAA82550.039.030898
ACGTTAG356.247734E-638.571431