FastQCFastQC Report
Thu 26 May 2016
SRR1546956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546956_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences867248
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.42986550559932113No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.24479733594081507No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11260.12983598693799236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG302.164883E-645.00000421
ACGGGTA600.045.0000045
TCTTGCG207.0323277E-445.01
CGAACTA207.0323277E-445.06
CGTCTAC207.0323277E-445.039
CTACGCG207.0323277E-445.01
ATACCGA253.8900114E-545.02
CGTTTTT36100.044.1274221
CGACGGT1350.043.33333228
CACGACG1350.043.33333226
TATGCGA700.041.7857132
GAGCGAT6200.041.3709687
CTTGCGA603.6379788E-1241.2500042
CGGAGAT603.6379788E-1241.2500045
CGCGTAA556.002665E-1140.90909231
GAGGGGA62650.040.3312037
AGCGATC451.9274012E-840.08
TGGTACG451.9274012E-840.01
AGGGGAA64050.039.414528
TGCACGA403.4569166E-739.3752