##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546956_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	867248
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69931668911315	31.0	31.0	33.0	30.0	34.0
2	32.07088053244286	33.0	31.0	34.0	30.0	34.0
3	32.11734705643599	33.0	31.0	34.0	30.0	34.0
4	35.87895042709813	37.0	35.0	37.0	35.0	37.0
5	35.87145891371326	37.0	35.0	37.0	35.0	37.0
6	35.91820332822906	37.0	35.0	37.0	35.0	37.0
7	36.10741679427338	37.0	35.0	37.0	35.0	37.0
8	13.289874407320628	0.0	0.0	35.0	0.0	37.0
9	24.98029514049038	18.0	17.0	39.0	17.0	39.0
10	32.47016193753113	32.0	32.0	37.0	27.0	39.0
11	35.48151393834289	37.0	34.0	37.0	32.0	39.0
12	36.628068326476395	37.0	35.0	39.0	33.0	39.0
13	36.91684500857886	39.0	37.0	39.0	33.0	39.0
14	38.09695842481044	40.0	37.0	41.0	33.0	41.0
15	38.38413925428482	40.0	37.0	41.0	34.0	41.0
16	38.464974263417155	40.0	38.0	41.0	34.0	41.0
17	38.45006733944616	40.0	37.0	41.0	34.0	41.0
18	38.39950856041178	40.0	37.0	41.0	34.0	41.0
19	38.37673537442577	40.0	37.0	41.0	34.0	41.0
20	38.34309217202	40.0	37.0	41.0	34.0	41.0
21	38.28228257661015	40.0	37.0	41.0	34.0	41.0
22	38.23218848587717	40.0	37.0	41.0	34.0	41.0
23	38.11677397930004	40.0	37.0	41.0	34.0	41.0
24	38.01564027821338	40.0	36.0	41.0	34.0	41.0
25	37.909871224839954	40.0	36.0	41.0	33.0	41.0
26	37.84815070199066	40.0	36.0	41.0	33.0	41.0
27	37.802159243953284	40.0	36.0	41.0	33.0	41.0
28	37.75091553973027	40.0	36.0	41.0	33.0	41.0
29	37.764642870320834	40.0	36.0	41.0	33.0	41.0
30	37.68510506798517	40.0	36.0	41.0	33.0	41.0
31	37.60181747320259	40.0	36.0	41.0	33.0	41.0
32	37.45315411508588	40.0	36.0	41.0	33.0	41.0
33	37.30518029444865	40.0	36.0	41.0	33.0	41.0
34	37.137137243326016	40.0	35.0	41.0	32.0	41.0
35	36.99479041750457	40.0	35.0	41.0	31.0	41.0
36	36.86957133369002	40.0	35.0	41.0	31.0	41.0
37	36.802630850690925	40.0	35.0	41.0	31.0	41.0
38	36.7323914266738	40.0	35.0	41.0	31.0	41.0
39	36.654421803221226	40.0	35.0	41.0	31.0	41.0
40	36.55590211796395	40.0	35.0	41.0	30.0	41.0
41	36.51207151818165	40.0	35.0	41.0	30.0	41.0
42	36.459538678670924	39.0	35.0	41.0	30.0	41.0
43	36.37434620777448	39.0	35.0	41.0	30.0	41.0
44	36.145901748980684	39.0	35.0	41.0	30.0	41.0
45	36.088882303562535	39.0	35.0	40.0	29.0	41.0
46	36.06623595557441	39.0	35.0	40.0	29.0	41.0
47	36.030507997712306	39.0	35.0	40.0	29.0	41.0
48	35.958071970186154	39.0	35.0	40.0	29.0	41.0
49	35.86870883530432	39.0	35.0	40.0	29.0	41.0
50	35.759360644244786	38.0	35.0	40.0	28.0	41.0
51	35.388715799863476	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	2.0
15	12.0
16	32.0
17	92.0
18	217.0
19	416.0
20	768.0
21	1370.0
22	2262.0
23	3462.0
24	5495.0
25	8083.0
26	10408.0
27	11341.0
28	11658.0
29	12953.0
30	15792.0
31	20445.0
32	27277.0
33	37740.0
34	58183.0
35	88287.0
36	92163.0
37	159445.0
38	208796.0
39	90520.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75920152021106	23.415793406269025	28.308857443314945	14.516147630204971
2	32.75902625315942	24.622368688079995	29.76438112281608	12.854223935944505
3	30.365823847388523	24.363503865099716	32.22769034924266	13.042981938269099
4	26.389221998782354	26.6961699536926	32.011604523734846	14.903003523790195
5	23.346147814696604	32.13313838717414	30.320392782687307	14.200321015441949
6	21.252974927587033	41.27319982288803	27.084986070881683	10.388839178643249
7	86.8655793959744	4.1130103499806285	7.3884286847591465	1.6329815692858327
8	33.01420124347361	63.510668228695835	2.6192046565688245	0.8559258712617384
9	84.0464319318119	4.431834953784846	8.743750345921812	2.7779827684814493
10	42.57559544674649	23.422596535247127	21.99440067892921	12.007407339077172
11	39.15857978340682	24.09195524232976	21.4332001918713	15.31626478239212
12	33.73049000977806	23.275695072228476	26.2740300352379	16.71978488275557
13	23.666817334833866	31.705117797907867	26.89611276128628	17.731952105971992
14	19.389148202129032	34.177420991458035	29.48464568381824	16.94878512259469
15	18.544522443407192	25.580802723096507	39.18244838846559	16.692226445030716
16	20.486527498477944	22.077421913916204	38.50409571425936	18.931954873346495
17	21.270501632750953	22.063354426876742	29.193264210468055	27.47287972990425
18	23.647791635149346	24.382529564784235	31.812122945224434	20.157555854841984
19	26.96979410733723	26.114329465158754	26.93877645148792	19.97709997601609
20	28.3689325314097	25.53929210560301	26.341830710477282	19.74994465251001
21	25.011530727081528	26.366967695515008	29.51162758518901	19.109873992214453
22	24.299277715255613	22.892759625850967	29.14967806209988	23.658284596793536
23	21.574912827703262	27.64537940704389	28.2027747541649	22.576933011087945
24	21.322620519159454	25.700261055661127	33.9551085733262	19.02200985185322
25	21.074825194177443	26.307584451045145	31.809586185266497	20.808004169510912
26	19.76839381584045	31.226707931295316	27.401158607457155	21.60373964540708
27	19.958304890873197	29.96132594136856	30.30736306108518	19.773006106673062
28	18.365334944560264	27.718138294928323	35.50368522037526	18.412841540136153
29	19.12359555744147	26.564719664963192	34.24199306311459	20.069691714480747
30	21.359749460361975	27.286081951183515	32.1940206261646	19.160147962289912
31	27.085447299964947	26.02750309023486	26.713350737044074	20.17369887275612
32	26.20092522554102	27.472649115362618	27.11219858679409	19.214227072302272
33	25.046353522867737	26.9876667343136	27.477953249820118	20.488026492998543
34	20.681512093426562	28.157574304005312	29.23777281700275	21.923140785565376
35	21.406448905042154	27.101129088795822	30.265045292695973	21.227376713466047
36	26.58582089552239	26.454716528605427	27.069188974779994	19.89027360109219
37	21.654359537294983	29.774528162647822	27.81119126247625	20.759921037580945
38	21.866294301053447	31.390213641311366	24.901642897994574	21.84184915964061
39	21.427319521059722	28.843767872626973	27.233501835691754	22.49541077062155
40	23.05107650868033	26.041685884545135	27.585650240761584	23.321587366012952
41	19.87228566684501	25.755608545652454	28.391763371031125	25.980342416471412
42	22.540957142593584	27.21839658321495	26.080429127539066	24.1602171466524
43	22.769150231537	27.0382866262015	26.57429016844086	23.618272973820638
44	20.98154161208789	29.107360293710684	27.719867903990554	22.191230190210874
45	20.606101138313377	31.831609873992218	25.46272807778167	22.099560909912736
46	23.305674962640445	29.405083666955704	26.975098241794736	20.314143128609118
47	22.62847536114237	28.37366012951313	27.119924173938713	21.87794033540579
48	22.714379277899745	26.882391196059256	28.762706861243842	21.640522664797153
49	21.800453849417927	26.185012822168513	29.591766138405625	22.422767190007935
50	20.837638138110435	29.548641219120714	28.354865044370236	21.25885559839861
51	20.70226740217331	31.145877534453813	26.358550264745496	21.793304798627382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	561.0
1	931.0
2	1301.0
3	5716.5
4	10132.0
5	7141.5
6	4151.0
7	4230.5
8	4310.0
9	4421.0
10	4532.0
11	4497.0
12	4462.0
13	4360.0
14	4258.0
15	4032.5
16	3807.0
17	3561.5
18	3316.0
19	3401.0
20	3486.0
21	3749.5
22	4013.0
23	4324.0
24	4635.0
25	5218.5
26	6892.5
27	7983.0
28	9974.5
29	11966.0
30	13897.0
31	15828.0
32	18087.0
33	20346.0
34	23398.5
35	26451.0
36	29472.5
37	32494.0
38	38572.0
39	44650.0
40	53462.5
41	62275.0
42	67050.0
43	71825.0
44	73097.0
45	74369.0
46	73795.0
47	73221.0
48	70569.5
49	67918.0
50	65291.0
51	62664.0
52	57234.0
53	51804.0
54	47618.0
55	43432.0
56	40069.0
57	36706.0
58	33248.0
59	29790.0
60	26991.5
61	24193.0
62	20793.0
63	17393.0
64	14452.5
65	11512.0
66	9481.5
67	7451.0
68	6444.0
69	5437.0
70	4515.5
71	3594.0
72	2992.0
73	2390.0
74	1916.5
75	1078.5
76	714.0
77	526.5
78	339.0
79	252.0
80	165.0
81	128.0
82	91.0
83	57.5
84	24.0
85	15.5
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	867248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.118572464263803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46471972917455	19.251301829503063
2	6.5803298649959805	3.7005896429529748
3	3.571318630360233	3.012611451022787
4	2.6096455530896954	2.935180303623856
5	2.147977932559272	3.019903657415372
6	1.7487568548618764	2.9503527807487036
7	1.545442334570613	3.0418942591775364
8	1.4304344170768022	3.2177419049560814
9	1.2440749458598963	3.148345036452291
>10	10.566450591681225	52.727568096072396
>50	0.07061456497240824	1.25405098629138
>100	0.018995728911833567	0.9303199636326577
>500	0.0	0.0
>1k	0.0012388518855543436	0.8101400881508976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3728	0.42986550559932113	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.24479733594081507	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1126	0.12983598693799236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009109274394406213	0.0
2	0.0	0.0	0.0	0.026174750475065955	0.0
3	0.0	0.0	0.0	0.0413953102226814	0.0
4	0.0	0.0	0.0	0.06134346807372286	0.0
5	0.0	0.0	0.0	0.11104090179510359	0.0
6	0.0	0.0	0.0	0.16961699536926	0.0
7	0.0	0.0	0.0	0.2054775565928085	0.0
8	0.0	0.0	0.0	0.28273342803903845	0.0
9	0.0	0.0	0.0	0.37163533383761044	0.0
10	0.0	0.0	0.0	0.5531289781008432	0.0
11	0.0	0.0	0.0	0.7247061970739627	0.0
12	1.1530727081526852E-4	0.0	0.0	0.8800250908621294	0.0
13	1.1530727081526852E-4	0.0	0.0	0.9441359334354187	0.0
14	1.1530727081526852E-4	0.0	0.0	0.972155600243529	0.0
15	1.1530727081526852E-4	0.0	0.0	1.0143580613619172	0.0
16	1.1530727081526852E-4	0.0	0.0	1.104413039868642	0.0
17	1.1530727081526852E-4	0.0	0.0	1.2068058963526005	0.0
18	1.1530727081526852E-4	0.0	0.0	1.3383714923528218	0.0
19	1.1530727081526852E-4	0.0027673744995664446	0.0	1.4095160784458425	0.0
20	1.1530727081526852E-4	0.0027673744995664446	0.0	1.4800841281847867	0.0
21	1.1530727081526852E-4	0.0027673744995664446	0.0	1.5765963138571666	0.0
22	1.1530727081526852E-4	0.0027673744995664446	0.0	1.672070734092209	0.0
23	1.1530727081526852E-4	0.0027673744995664446	0.0	1.7874933121782928	0.0
24	1.1530727081526852E-4	0.0027673744995664446	0.0	1.8746656089146356	0.0
25	1.1530727081526852E-4	0.0027673744995664446	0.0	1.9528439385273877	0.0
26	1.1530727081526852E-4	0.0027673744995664446	0.0	2.036211095326827	0.0
27	1.1530727081526852E-4	0.0027673744995664446	0.0	2.1130057376897957	0.0
28	1.1530727081526852E-4	0.0027673744995664446	0.0	2.2001780344261386	0.0
29	1.1530727081526852E-4	0.0027673744995664446	0.0	2.293922845598952	0.0
30	1.1530727081526852E-4	0.0027673744995664446	0.0	2.4026576019777504	0.0
31	1.1530727081526852E-4	0.0027673744995664446	0.0	2.5043586148368173	0.0
32	1.1530727081526852E-4	0.0027673744995664446	0.0	2.6029463313838717	0.0
33	1.1530727081526852E-4	0.0027673744995664446	0.0	2.7045320369721235	0.0
34	1.1530727081526852E-4	0.0027673744995664446	0.0	2.810499418851355	0.0
35	1.1530727081526852E-4	0.0027673744995664446	0.0	2.9322638968322785	0.0
36	1.1530727081526852E-4	0.0027673744995664446	0.0	3.041113960481892	0.0
37	1.1530727081526852E-4	0.0027673744995664446	0.0	3.155729387672269	0.0
38	1.1530727081526852E-4	0.0027673744995664446	0.0	3.2812990055900966	0.0
39	1.1530727081526852E-4	0.0027673744995664446	0.0	3.436617899378263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.164883E-6	45.000004	21
ACGGGTA	60	0.0	45.000004	5
TCTTGCG	20	7.0323277E-4	45.0	1
CGAACTA	20	7.0323277E-4	45.0	6
CGTCTAC	20	7.0323277E-4	45.0	39
CTACGCG	20	7.0323277E-4	45.0	1
ATACCGA	25	3.8900114E-5	45.0	2
CGTTTTT	3610	0.0	44.127422	1
CGACGGT	135	0.0	43.333332	28
CACGACG	135	0.0	43.333332	26
TATGCGA	70	0.0	41.785713	2
GAGCGAT	620	0.0	41.370968	7
CTTGCGA	60	3.6379788E-12	41.250004	2
CGGAGAT	60	3.6379788E-12	41.250004	5
CGCGTAA	55	6.002665E-11	40.909092	31
GAGGGGA	6265	0.0	40.331203	7
AGCGATC	45	1.9274012E-8	40.0	8
TGGTACG	45	1.9274012E-8	40.0	1
AGGGGAA	6405	0.0	39.41452	8
TGCACGA	40	3.4569166E-7	39.375	2
>>END_MODULE