FastQCFastQC Report
Thu 26 May 2016
SRR1546955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546955_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1305618
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101340.7761841518729061No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.41650773809797353No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.03364E-445.01
GTTACGA351.2121018E-745.02
CGTACAT207.03364E-445.043
CGTAAGA1350.045.02
CGAGTTA302.1657306E-644.9999966
TCGAGTA302.1657306E-644.9999965
TAGTGCG600.044.9999961
CGTTTTT80100.043.7078631
TATGCGA750.042.02
GTTTTTA58350.041.375322
TAATGCG556.184564E-1140.9090921
GACCGAT3100.040.645169
TACGAGT501.0822987E-940.54
ACGACGG3150.040.027
AGCGATA1700.039.7058838
GCGCGAC7150.039.650359
AGGGGAA84650.039.551098
CGACGGT3250.039.4615428
AGACTTA10450.039.4019138
CGGTCTA3250.038.7692331
Produced by FastQC (version 0.11.5)