##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546955_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1305618
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714584204568258	31.0	31.0	34.0	30.0	34.0
2	32.07093728793568	33.0	31.0	34.0	30.0	34.0
3	32.06145135866693	33.0	31.0	34.0	30.0	34.0
4	35.835768195597794	37.0	35.0	37.0	35.0	37.0
5	35.82840846250588	37.0	35.0	37.0	35.0	37.0
6	35.8677591761143	37.0	35.0	37.0	35.0	37.0
7	36.09137588483002	37.0	35.0	37.0	35.0	37.0
8	13.247287491440835	0.0	0.0	35.0	0.0	37.0
9	24.933132049343683	18.0	17.0	39.0	17.0	39.0
10	32.665714627096136	32.0	32.0	38.0	27.0	39.0
11	35.550164749566875	37.0	35.0	37.0	32.0	39.0
12	36.61882342308393	37.0	35.0	39.0	33.0	39.0
13	36.85700028645439	39.0	35.0	39.0	33.0	39.0
14	38.010990197745436	40.0	37.0	41.0	33.0	41.0
15	38.291751492396706	40.0	37.0	41.0	33.0	41.0
16	38.3724803120055	40.0	37.0	41.0	34.0	41.0
17	38.34340902162807	40.0	37.0	41.0	34.0	41.0
18	38.28287447017428	40.0	37.0	41.0	34.0	41.0
19	38.24703703533499	40.0	37.0	41.0	34.0	41.0
20	38.2021058226832	40.0	37.0	41.0	34.0	41.0
21	38.14323791491845	40.0	37.0	41.0	34.0	41.0
22	38.09144711546563	40.0	36.0	41.0	33.0	41.0
23	37.96277241888516	40.0	36.0	41.0	33.0	41.0
24	37.82797035580086	40.0	35.0	41.0	33.0	41.0
25	37.71461254363834	40.0	35.0	41.0	33.0	41.0
26	37.63665023000602	40.0	35.0	41.0	33.0	41.0
27	37.59632066959861	40.0	35.0	41.0	33.0	41.0
28	37.50632114446952	40.0	35.0	41.0	33.0	41.0
29	37.4772567473794	40.0	35.0	41.0	33.0	41.0
30	37.354613677201144	40.0	35.0	41.0	33.0	41.0
31	37.25538940180053	40.0	35.0	41.0	33.0	41.0
32	37.07925059243975	40.0	35.0	41.0	32.0	41.0
33	36.877947454768545	40.0	35.0	41.0	31.0	41.0
34	36.62986646936547	40.0	35.0	41.0	31.0	41.0
35	36.44586624878027	40.0	35.0	41.0	30.0	41.0
36	36.33269915090019	39.0	35.0	41.0	30.0	41.0
37	36.28067857520347	39.0	35.0	41.0	30.0	41.0
38	36.18375818960829	39.0	35.0	41.0	30.0	41.0
39	36.10354560062744	39.0	35.0	41.0	29.0	41.0
40	36.0003423666034	39.0	35.0	41.0	29.0	41.0
41	35.92459050043734	39.0	35.0	41.0	29.0	41.0
42	35.86606419335518	39.0	35.0	41.0	28.0	41.0
43	35.76628079576109	39.0	35.0	40.0	28.0	41.0
44	35.52026013734492	38.0	35.0	40.0	27.0	41.0
45	35.463001429208234	38.0	35.0	40.0	26.0	41.0
46	35.452966334716585	38.0	35.0	40.0	27.0	41.0
47	35.405002841566215	38.0	35.0	40.0	27.0	41.0
48	35.32582347976207	38.0	35.0	40.0	26.0	41.0
49	35.23954479794243	38.0	34.0	40.0	26.0	41.0
50	35.119292932542294	38.0	34.0	40.0	26.0	41.0
51	34.766725029832614	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	4.0
14	11.0
15	23.0
16	64.0
17	127.0
18	354.0
19	710.0
20	1403.0
21	2463.0
22	3847.0
23	6266.0
24	9480.0
25	14573.0
26	19044.0
27	21022.0
28	21626.0
29	23318.0
30	27246.0
31	34023.0
32	44393.0
33	61090.0
34	92777.0
35	136392.0
36	139961.0
37	231479.0
38	292971.0
39	120914.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.19202094333871	22.441020267796553	27.826745648420903	16.540213140443836
2	34.73098563285739	24.184332630218027	28.323904848125565	12.760776888799022
3	29.71389793951983	23.94513556032469	33.04710872552308	13.293857774632395
4	26.296435864088885	26.014270636587423	32.52329548152675	15.165998017796936
5	23.240641596546617	30.264518411970425	31.56015005920568	14.934689932277282
6	21.47197725521554	38.689800538901885	29.18602531521471	10.652196890667867
7	83.77059752546305	4.209271011888623	9.810909469691747	2.209221992956592
8	31.668604446323506	63.626497183709176	3.6031978725783502	1.101700497388976
9	80.23311565863828	4.959950000689329	11.241036811686113	3.5658975289862735
10	45.248916605010045	24.373898031430326	17.19331381767102	13.183871545888614
11	38.410852178814935	21.757512534294104	24.128879963358347	15.70275532353261
12	34.18450113279688	20.595151108517193	28.284689702501037	16.935658056184888
13	24.809477197771475	27.43528352090734	29.344877291826556	18.410361989494632
14	18.63669158972992	30.608493449079287	32.177099274060254	18.57771568713054
15	18.769655442863073	23.46536276307465	40.38616195548775	17.37881983857453
16	22.27313042559156	20.216326674417786	38.44999073235817	19.060552167632494
17	21.762950572066256	20.796205321924177	30.556104465471524	26.884739640538047
18	24.131254317878582	23.784215597517804	31.974360034864713	20.110170049738898
19	27.200222423404092	25.55295653093018	27.96307955313116	19.28374149253457
20	28.652944429381332	25.302270648842157	26.409102815678093	19.635682106098415
21	25.610323999822306	25.601209542147856	29.980132014111327	18.80833444391851
22	23.41902455388942	22.553916995629656	30.238400512247836	23.788657938233083
23	22.246246605055997	25.823096801667873	29.51115870032429	22.419497892951842
24	23.35537653433087	24.497824018970327	32.82529805808437	19.321501388614433
25	22.994244871011276	23.550226789152724	31.701768817525494	21.753759522310506
26	19.949173494850715	27.829732739591517	29.084311031251104	23.136782734306667
27	20.23003665697011	26.746567525876635	31.285873816077903	21.73752200107535
28	18.926362841198575	26.486996962358056	34.38838925321189	20.19825094323148
29	20.823931655354016	25.731033119947792	33.120483939406476	20.32455128529172
30	22.38181458895328	25.366914365457582	32.16285314693884	20.08841789865029
31	27.661996081549123	23.720797354203143	28.21874392050355	20.39846264374419
32	28.271975417005585	24.78420181094317	27.870326542679408	19.073496229371838
33	26.984615714550504	24.286046914181636	27.509884208091496	21.219453163176365
34	21.96844712618852	26.376934141532978	30.39411221352647	21.260506518752038
35	21.836172601787045	25.573866169124504	30.0502903605802	22.539670868508246
36	26.390567532004	26.33480849681913	27.350496086910564	19.9241278842663
37	22.591600299628222	28.836459056171098	27.53217250374918	21.039768140451496
38	22.81065365214021	29.368850613272794	25.114543457581007	22.705952277005988
39	22.362666568628804	26.913078710618265	26.724585598544138	23.999669122208793
40	23.52012610120265	23.947739691088817	27.83700898731482	24.695125220393713
41	20.471837857627577	24.123518517667495	28.530780059711187	26.873863564993744
42	22.228553834276184	26.683072690480675	26.477346360114524	24.61102711512862
43	22.194623542261212	24.837892859932996	27.589233604316117	25.37824999348967
44	21.575606341211596	26.342161336623732	29.163047690825337	22.919184631339334
45	21.792438523365945	28.185962509708045	26.832657025255475	23.188941941670535
46	23.06555209869962	27.0832663152622	28.371851491018045	21.479330095020135
47	22.19983180378947	26.310681991210295	28.179145814472534	23.310340390527703
48	22.17034385248978	25.05763554117667	30.110568328561648	22.661452277771907
49	22.837614064757073	23.80397635449266	30.279836828230007	23.078572752520262
50	20.873333547791162	26.610233621166373	29.076039086470928	23.440393744571537
51	19.79185335986483	28.4538050180068	28.684806735201263	23.06953488692711
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1024.0
1	1938.0
2	2852.0
3	14835.5
4	26819.0
5	18072.0
6	9325.0
7	9130.0
8	8935.0
9	8868.5
10	8802.0
11	8456.0
12	8110.0
13	7665.0
14	7220.0
15	6807.5
16	6395.0
17	6027.5
18	5660.0
19	5530.0
20	5400.0
21	5635.0
22	5870.0
23	6141.5
24	6413.0
25	7184.5
26	9632.0
27	11308.0
28	13193.5
29	15079.0
30	18040.5
31	21002.0
32	22284.5
33	23567.0
34	25898.5
35	28230.0
36	32035.5
37	35841.0
38	44100.5
39	52360.0
40	62904.0
41	73448.0
42	80336.0
43	87224.0
44	91223.0
45	95222.0
46	98681.5
47	102141.0
48	101358.0
49	100575.0
50	96840.5
51	93106.0
52	87948.0
53	82790.0
54	78803.5
55	74817.0
56	71288.5
57	67760.0
58	64815.0
59	61870.0
60	56655.0
61	51440.0
62	45417.5
63	39395.0
64	33047.5
65	26700.0
66	22252.0
67	17804.0
68	15030.0
69	12256.0
70	10577.5
71	8899.0
72	7422.5
73	5946.0
74	4611.0
75	2442.0
76	1608.0
77	1140.5
78	673.0
79	469.0
80	265.0
81	197.0
82	129.0
83	97.5
84	66.0
85	44.5
86	23.0
87	15.5
88	8.0
89	5.0
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1305618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.33872367586466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60296265743082	21.007395357796188
2	6.594705964934196	3.8696051205756157
3	3.291799733448584	2.8973160832779885
4	2.2602808787361592	2.6525502452432295
5	1.8164486988080875	2.6646143222857197
6	1.534897329824738	2.7019157178330295
7	1.2700674960180516	2.608351152075967
8	1.1482414108285766	2.6950349972387677
9	1.041222488274932	2.7493324981735014
>10	9.294057435584698	50.75602361427227
>50	0.11136249283525435	2.01666594723781
>100	0.03158457055297923	1.7774710140294108
>500	0.001842433228964499	0.4012398661438335
>1k	0.0	0.0
>5k	2.6320474699492843E-4	0.4199273271921028
>10k+	2.6320474699492843E-4	0.782556736624636
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10134	0.7761841518729061	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5438	0.41650773809797353	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014399311284004969	0.0
2	0.0	0.0	0.0	0.04227882887644012	0.0
3	0.0	0.0	0.0	0.07322202972079123	0.0
4	0.0	0.0	0.0	0.11098192580065531	0.0
5	0.0	0.0	0.0	0.2061092907726456	0.0
6	0.0	0.0	0.0	0.3481110094989499	0.0
7	0.0	0.0	0.0	0.44247245365796123	0.0
8	0.0	0.0	0.0	0.6134259791148713	0.0
9	0.0	0.0	0.0	0.8821875923891981	0.0
10	0.0	0.0	0.0	1.2944061739344892	0.0
11	0.0	0.0	0.0	1.660822690863637	0.0
12	0.0	0.0	0.0	1.9547065068036746	0.0
13	0.0	0.0	0.0	2.0820025459207825	0.0
14	0.0	0.0	0.0	2.1467994466988047	0.0
15	0.0	0.0	0.0	2.222089462614639	0.0
16	0.0	0.0	0.0	2.3441772402034897	0.0
17	0.0	0.0	0.0	2.4838046044095594	0.0
18	0.0	0.0	0.0	2.679803740450882	0.0
19	0.0	0.0	0.0	2.786496509698855	0.0
20	0.0	0.0	0.0	2.905980156523577	0.0
21	0.0	0.0	0.0	3.0474457306807965	0.0
22	0.0	0.0	0.0	3.1978725783498696	0.0
23	0.0	0.0	0.0	3.3568011470430097	0.0
24	0.0	0.0	0.0	3.4801143979326263	0.0
25	0.0	0.0	0.0	3.5897942583512177	0.0
26	0.0	0.0	0.0	3.7004698158266813	0.0
27	0.0	0.0	0.0	3.8136729119849755	0.0
28	0.0	0.0	0.0	3.931548125102442	0.0
29	0.0	0.0	0.0	4.0647417544794875	0.0
30	0.0	0.0	0.0	4.212411287221837	0.0
31	0.0	0.0	0.0	4.348209047363011	0.0
32	0.0	0.0	0.0	4.4761944152118005	0.0
33	0.0	0.0	0.0	4.615285634848784	0.0
34	0.0	0.0	0.0	4.764180640891899	0.0
35	0.0	0.0	0.0	4.922649657097252	0.0
36	0.0	0.0	0.0	5.072846728522432	0.0
37	0.0	0.0	0.0	5.234456020060998	0.0
38	0.0	0.0	0.0	5.428080801582086	0.0
39	0.0	0.0	0.0	5.709786476595758	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.03364E-4	45.0	1
GTTACGA	35	1.2121018E-7	45.0	2
CGTACAT	20	7.03364E-4	45.0	43
CGTAAGA	135	0.0	45.0	2
CGAGTTA	30	2.1657306E-6	44.999996	6
TCGAGTA	30	2.1657306E-6	44.999996	5
TAGTGCG	60	0.0	44.999996	1
CGTTTTT	8010	0.0	43.707863	1
TATGCGA	75	0.0	42.0	2
GTTTTTA	5835	0.0	41.37532	2
TAATGCG	55	6.184564E-11	40.909092	1
GACCGAT	310	0.0	40.64516	9
TACGAGT	50	1.0822987E-9	40.5	4
ACGACGG	315	0.0	40.0	27
AGCGATA	170	0.0	39.705883	8
GCGCGAC	715	0.0	39.65035	9
AGGGGAA	8465	0.0	39.55109	8
CGACGGT	325	0.0	39.46154	28
AGACTTA	1045	0.0	39.401913	8
CGGTCTA	325	0.0	38.76923	31
>>END_MODULE