##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546954_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	766222
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.723664943058278	31.0	31.0	34.0	30.0	34.0
2	32.06838879593643	33.0	31.0	34.0	30.0	34.0
3	32.0983057129657	33.0	31.0	34.0	30.0	34.0
4	35.83501648347346	37.0	35.0	37.0	35.0	37.0
5	35.80894440514629	37.0	35.0	37.0	35.0	37.0
6	35.85750343895111	37.0	35.0	37.0	35.0	37.0
7	36.087229288639584	37.0	35.0	37.0	35.0	37.0
8	13.25152501494345	0.0	0.0	35.0	0.0	37.0
9	24.948727653343287	18.0	17.0	39.0	17.0	39.0
10	32.7121082401706	32.0	32.0	38.0	27.0	39.0
11	35.59613401860035	37.0	35.0	37.0	32.0	39.0
12	36.507590489440396	37.0	35.0	39.0	33.0	39.0
13	36.5900691966558	39.0	35.0	39.0	33.0	39.0
14	37.70908300727466	39.0	36.0	41.0	33.0	41.0
15	38.07667360112343	40.0	37.0	41.0	33.0	41.0
16	38.21790551563385	40.0	37.0	41.0	34.0	41.0
17	38.21761056195202	40.0	36.0	41.0	34.0	41.0
18	38.18046336440353	40.0	36.0	41.0	34.0	41.0
19	38.143411439504476	40.0	36.0	41.0	34.0	41.0
20	38.11054368055211	40.0	36.0	41.0	34.0	41.0
21	38.01178770643495	40.0	36.0	41.0	33.0	41.0
22	37.95314934836118	40.0	36.0	41.0	33.0	41.0
23	37.8797789674533	40.0	36.0	41.0	33.0	41.0
24	37.787153070520034	40.0	35.0	41.0	33.0	41.0
25	37.685229607085155	40.0	35.0	41.0	33.0	41.0
26	37.59073088478274	40.0	35.0	41.0	33.0	41.0
27	37.53132120977993	40.0	35.0	41.0	33.0	41.0
28	37.50835919615986	40.0	35.0	41.0	33.0	41.0
29	37.52614646930002	40.0	35.0	41.0	33.0	41.0
30	37.44430073790625	40.0	35.0	41.0	33.0	41.0
31	37.34077330068831	40.0	35.0	41.0	33.0	41.0
32	37.20384562176497	40.0	35.0	41.0	32.0	41.0
33	37.07605628655925	40.0	35.0	41.0	32.0	41.0
34	36.935285074038596	40.0	35.0	41.0	31.0	41.0
35	36.81137059494507	40.0	35.0	41.0	31.0	41.0
36	36.68321191508466	40.0	35.0	41.0	31.0	41.0
37	36.60232282549966	39.0	35.0	41.0	31.0	41.0
38	36.52660978149936	39.0	35.0	41.0	31.0	41.0
39	36.45540065411852	39.0	35.0	41.0	30.0	41.0
40	36.31642657088938	39.0	35.0	41.0	30.0	41.0
41	36.285742252245434	39.0	35.0	41.0	30.0	41.0
42	36.23792190774997	39.0	35.0	41.0	30.0	41.0
43	36.13756979047848	39.0	35.0	41.0	30.0	41.0
44	35.95376535782058	38.0	35.0	40.0	29.0	41.0
45	35.83579954634558	38.0	35.0	40.0	29.0	41.0
46	35.82545920111926	38.0	35.0	40.0	29.0	41.0
47	35.780574037289455	38.0	35.0	40.0	29.0	41.0
48	35.7114726541394	38.0	35.0	40.0	29.0	41.0
49	35.62609530919237	38.0	35.0	40.0	29.0	41.0
50	35.500788283291264	38.0	35.0	40.0	28.0	41.0
51	35.095490601940426	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	6.0
15	15.0
16	32.0
17	91.0
18	212.0
19	426.0
20	884.0
21	1299.0
22	2108.0
23	3314.0
24	5113.0
25	6974.0
26	8970.0
27	9947.0
28	10685.0
29	12209.0
30	15071.0
31	19558.0
32	26236.0
33	36472.0
34	58075.0
35	90254.0
36	80254.0
37	135662.0
38	177696.0
39	64638.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.06075523803806	22.710389417166304	29.298036339337685	13.930819005457948
2	30.99897940805667	24.778588972908636	30.89718123468133	13.32525038435336
3	30.89718123468133	24.17393392515485	31.980679228735276	12.948205611428543
4	27.186376794192807	26.759607528888495	31.013596581669546	15.040419095249158
5	23.953762747611005	32.04319374802603	29.458955759557938	14.544087744805031
6	21.34929563494653	41.06590518152702	27.03433208652323	10.550467097003219
7	86.21717987737235	5.110920855835515	6.738386525054096	1.9335127417380342
8	32.84883493295677	63.754760369710084	2.3597599651276004	1.0366447322055488
9	84.28458070898512	4.966445755929744	7.806745303580424	2.9422282315047075
10	52.36993978246514	22.167857357267216	13.394159917099744	12.068042943167907
11	46.94514122538899	20.439246067066723	18.624106329497195	13.991506378047095
12	41.481581056143	21.023019438230694	22.82262842883655	14.672771076789756
13	23.104661573277717	37.72875224151747	23.857707035297864	15.308879149906945
14	16.714346494880076	40.005246521243194	27.52713965404282	15.753267329833912
15	15.697669865913536	23.358634964801325	45.64460430527967	15.299090864005471
16	18.330066221016885	19.030385449647753	43.30141917094524	19.338129158390128
17	18.878601762935546	19.06927757229628	27.940335829563757	34.11178483520442
18	23.954154279047067	23.572150107932167	32.154388675866784	20.319306937153982
19	29.42554507701423	25.72896627870252	24.685665512083965	20.159823132199286
20	31.18391275635521	24.431300589124298	23.25918598004234	21.125600674478154
21	24.23161955673421	27.16340694994401	27.775892626418972	20.829080866902807
22	23.731242381450805	22.91594342109728	26.582504809311143	26.770309388140774
23	20.853355815938464	28.402342924113377	26.03736253983832	24.70693872010984
24	22.129356766054748	24.617669552688383	35.60325858563184	17.649715095625027
25	20.612563982762175	23.693916384546515	33.551764371161354	22.14175526152995
26	17.546481306984138	32.37886669920728	26.796411483878043	23.27824050993054
27	18.673830821876688	31.302024739566342	29.9749941922837	20.04915024627327
28	16.617116188258755	27.52296331872486	37.55895810874655	18.300962384269834
29	17.532777706722072	25.989073662724383	35.71288738772836	20.765261242825186
30	21.400847274027633	27.991365426730113	31.375371628588063	19.232415670654195
31	29.637363583922156	25.329995745358396	25.420178486130652	19.6124621845888
32	30.430345252420317	25.846556219998906	26.009433297399447	17.71366523018133
33	27.877821310273003	26.418583648081107	24.99588891992138	20.707706121724513
34	20.16465201991068	27.76571280908144	28.83446311904383	23.23517205196405
35	21.16449279712668	26.367032009000003	29.9635092701593	22.504965923714014
36	30.27347165703934	24.826747339543893	26.347716458154423	18.55206454526234
37	21.57181600110673	30.934376721106936	27.65960778990945	19.834199487876884
38	22.290928738668427	32.144730900443996	23.355763734270223	22.20857662661735
39	21.299310121609665	29.996919952703006	26.122716392899186	22.581053532788147
40	24.180459449089167	25.093902289414814	25.942481421833357	24.783156839662656
41	19.14797539094414	23.265711503976654	28.20318393363803	29.383129171441176
42	23.129067032792065	26.04649827334637	24.17041014223032	26.65402455163125
43	22.94361164257878	25.811840432668337	25.66253644505117	25.582011479701706
44	21.13930427474022	28.80862204426393	27.616017290028218	22.436056390967632
45	19.378326385825517	34.98751014718972	23.414101918243016	22.22006154874175
46	23.626703488023054	30.509695623461607	25.45907060877918	20.40453027973616
47	23.31543599635615	27.625936086408377	26.146469300020097	22.912158617215376
48	23.451427915147306	25.075761332877416	29.253923797541702	22.21888695443357
49	22.31141888382218	24.182939148184207	30.30570774527487	23.199934222718742
50	20.86484073806286	30.4390894544923	26.862449786093322	21.833620021351514
51	20.096786570993785	32.63062141259322	24.826616829065205	22.44597518734779
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	925.0
1	1280.5
2	1636.0
3	4120.5
4	6605.0
5	4831.5
6	3058.0
7	3246.0
8	3434.0
9	3634.5
10	3835.0
11	3765.5
12	3696.0
13	3549.0
14	3402.0
15	3284.5
16	3167.0
17	2956.0
18	2745.0
19	2820.5
20	2896.0
21	2979.5
22	3063.0
23	3294.0
24	3525.0
25	3632.0
26	4465.5
27	5192.0
28	6143.0
29	7094.0
30	8525.5
31	9957.0
32	11000.5
33	12044.0
34	14617.5
35	17191.0
36	20557.5
37	23924.0
38	31397.5
39	38871.0
40	49891.5
41	60912.0
42	65465.0
43	70018.0
44	69835.0
45	69652.0
46	68897.0
47	68142.0
48	63944.5
49	59747.0
50	55462.5
51	51178.0
52	47695.0
53	44212.0
54	41954.5
55	39697.0
56	37751.5
57	35806.0
58	33127.0
59	30448.0
60	27107.5
61	23767.0
62	20956.0
63	18145.0
64	15015.5
65	11886.0
66	9967.5
67	8049.0
68	6740.5
69	5432.0
70	4646.5
71	3861.0
72	3239.5
73	2618.0
74	1970.5
75	1012.5
76	702.0
77	543.0
78	384.0
79	272.5
80	161.0
81	102.5
82	44.0
83	34.0
84	24.0
85	14.5
86	5.0
87	5.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	766222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.291758172919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99360441963245	18.714283493239744
2	5.505487562177626	2.7848692009321976
3	2.4463582060625133	1.8561810045620715
4	1.6106218478801253	1.6294183313841626
5	1.3577816536942227	1.7170342618430166
6	1.0900039240117145	1.6540869392182238
7	0.9909193742937534	1.7543465228448474
8	0.875559889277564	1.7715559188412708
9	0.799756354956132	1.8204519894125093
>10	10.965782637072602	58.897288624507524
>50	0.3273491475888533	5.066577711755096
>100	0.034703153304430315	1.53964959784193
>500	5.179575120064226E-4	0.09209339464671801
>1k	0.001553872536019268	0.7021630089707092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2455	0.3204032251749493	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1616	0.2109049335571153	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.16822800702668417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011354411645711035	0.0
2	0.0	0.0	0.0	0.03667344451085978	0.0
3	0.0	0.0	0.0	0.05272623338927882	0.0
4	0.0	0.0	0.0	0.08078598630684057	0.0
5	0.0	0.0	0.0	0.1470853094794981	0.0
6	0.0	0.0	0.0	0.21338463265215565	0.0
7	0.0	0.0	1.3051047868633373E-4	0.25788870588419543	0.0
8	0.0	0.0	1.3051047868633373E-4	0.34689685234827505	0.0
9	0.0	0.0	1.3051047868633373E-4	0.4437356275335347	0.0
10	0.0	0.0	1.3051047868633373E-4	0.6147043546126318	0.0
11	0.0	0.0	1.3051047868633373E-4	0.8159515127469584	0.0
12	0.0	0.0	1.3051047868633373E-4	0.9814387997212296	0.0
13	0.0	0.0	1.3051047868633373E-4	1.0510008848610455	0.0
14	0.0	0.0020881676589813397	1.3051047868633373E-4	1.0790606377786072	0.0
15	0.0	0.0020881676589813397	1.3051047868633373E-4	1.1277410463286097	0.0
16	0.0	0.0020881676589813397	1.3051047868633373E-4	1.2269290101302233	0.0
17	0.0	0.0020881676589813397	1.3051047868633373E-4	1.3310763721219177	0.0
18	0.0	0.0020881676589813397	1.3051047868633373E-4	1.5051773506894868	0.0
19	0.0	0.0020881676589813397	1.3051047868633373E-4	1.5827005750291692	0.0
20	0.0	0.0020881676589813397	1.3051047868633373E-4	1.6595712469754196	0.0
21	0.0	0.0020881676589813397	1.3051047868633373E-4	1.771549237688294	0.0
22	0.0	0.0020881676589813397	1.3051047868633373E-4	1.872042306276771	0.0
23	0.0	0.0020881676589813397	1.3051047868633373E-4	1.9939390933698067	0.0
24	0.0	0.0020881676589813397	1.3051047868633373E-4	2.0795539673880414	0.0
25	0.0	0.0020881676589813397	1.3051047868633373E-4	2.1530313668884475	0.0
26	0.0	0.0020881676589813397	1.3051047868633373E-4	2.2293799969199526	0.0
27	0.0	0.0020881676589813397	1.3051047868633373E-4	2.311210067056284	0.0
28	0.0	0.0020881676589813397	1.3051047868633373E-4	2.3912129904910064	0.0
29	0.0	0.0020881676589813397	1.3051047868633373E-4	2.489226359984443	0.0
30	0.0	0.0020881676589813397	1.3051047868633373E-4	2.5992466935170224	0.0
31	0.0	0.0020881676589813397	1.3051047868633373E-4	2.707700901305366	0.0
32	0.0	0.0020881676589813397	1.3051047868633373E-4	2.7940988381957186	0.0
33	0.0	0.0020881676589813397	1.3051047868633373E-4	2.8909376133809785	0.0
34	0.0	0.0020881676589813397	1.3051047868633373E-4	2.993779870585809	0.0
35	0.0	0.0020881676589813397	1.3051047868633373E-4	3.1288582160261647	0.0
36	0.0	0.0020881676589813397	1.3051047868633373E-4	3.238356507643999	0.0
37	0.0	0.0020881676589813397	1.3051047868633373E-4	3.34224284867832	0.0
38	0.0	0.0020881676589813397	1.3051047868633373E-4	3.4705346492269866	0.0
39	0.0	0.0020881676589813397	1.3051047868633373E-4	3.6481594107190864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.031812E-4	45.000004	8
TCCGATC	20	7.031812E-4	45.000004	14
GACTTAG	40	6.8102963E-9	45.000004	1
TCGGATG	20	7.031812E-4	45.000004	13
GTATGCG	40	6.8102963E-9	45.000004	1
CGCTAGT	40	6.8102963E-9	45.000004	28
CGTAAGA	75	0.0	45.000004	2
ATCGGTA	20	7.031812E-4	45.000004	27
CGACTAT	20	7.031812E-4	45.000004	24
TAGTGCC	20	7.031812E-4	45.000004	38
TCGACGA	20	7.031812E-4	45.000004	2
GGTTACG	20	7.031812E-4	45.000004	20
TCGTTAG	25	3.8895843E-5	45.0	1
TCGCAAA	25	3.8895843E-5	45.0	27
AATTCGG	25	3.8895843E-5	45.0	13
TATAGCG	35	1.2112105E-7	45.0	1
CGCATGA	30	2.16455E-6	44.999996	2
GAGGGGA	8685	0.0	42.35751	7
AGGGGAA	8880	0.0	42.187504	8
ATACGAG	145	0.0	41.89655	3
>>END_MODULE