FastQCFastQC Report
Thu 26 May 2016
SRR1546953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546953_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1404562
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95300.6785033341354814No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.3763450812424087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA406.8175723E-945.039
CGATATT253.891249E-545.010
CGTTTTT84600.043.72341
GAGCGAT8050.041.9254657
CGAGGTA700.041.7857136
CACGACG2750.041.7272726
TATGCGA1550.040.645162
CGGTCTA2850.040.26315731
GTTTTTA60650.040.0659522
ATCGCAA451.9288564E-840.033
TATACGA800.039.3752
AGGGGAA92700.039.1262138
GAGGGGA92650.039.0016177
AGACTTA11250.039.08
ACGGTCT2950.038.89830430
TCGGACG356.24928E-638.5714261
ATCGGGT356.24928E-638.5714264
TCACGAC3000.038.25000425
AACGTAG659.094947E-1238.0769271
GAGACTT14200.037.711277
Produced by FastQC (version 0.11.5)