##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546953_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1404562
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69425130396522	31.0	31.0	33.0	30.0	34.0
2	32.060788345405896	33.0	31.0	34.0	30.0	34.0
3	32.072884643041746	33.0	31.0	34.0	30.0	34.0
4	35.84611715253581	37.0	35.0	37.0	35.0	37.0
5	35.85216530135373	37.0	35.0	37.0	35.0	37.0
6	35.89808424263222	37.0	35.0	37.0	35.0	37.0
7	36.1234057307545	37.0	35.0	37.0	35.0	37.0
8	13.273287330854743	0.0	0.0	36.0	0.0	37.0
9	24.987238726378756	18.0	17.0	39.0	17.0	39.0
10	32.5025310381457	32.0	32.0	37.0	27.0	39.0
11	35.48337417643365	37.0	34.0	37.0	32.0	39.0
12	36.634885466074124	37.0	35.0	39.0	33.0	39.0
13	36.92189308837915	39.0	37.0	39.0	33.0	39.0
14	38.09729865965333	40.0	37.0	41.0	33.0	41.0
15	38.37379766788508	40.0	38.0	41.0	34.0	41.0
16	38.44395904203588	40.0	38.0	41.0	34.0	41.0
17	38.430533504394965	40.0	37.0	41.0	34.0	41.0
18	38.37022858371507	40.0	37.0	41.0	34.0	41.0
19	38.33929865680547	40.0	37.0	41.0	34.0	41.0
20	38.29417213337681	40.0	37.0	41.0	34.0	41.0
21	38.22131810486116	40.0	37.0	41.0	34.0	41.0
22	38.18679488694696	40.0	37.0	41.0	34.0	41.0
23	38.05379043431333	40.0	37.0	41.0	34.0	41.0
24	37.90268852496365	40.0	36.0	41.0	34.0	41.0
25	37.800639630005655	40.0	35.0	41.0	33.0	41.0
26	37.744061137920575	40.0	35.0	41.0	33.0	41.0
27	37.67972293141919	40.0	35.0	41.0	33.0	41.0
28	37.61030057768899	40.0	35.0	41.0	33.0	41.0
29	37.60933586413416	40.0	35.0	41.0	33.0	41.0
30	37.50567223091611	40.0	35.0	41.0	33.0	41.0
31	37.4118842742435	40.0	35.0	41.0	33.0	41.0
32	37.224142472884786	40.0	35.0	41.0	32.0	41.0
33	37.0055455010174	40.0	35.0	41.0	31.0	41.0
34	36.773335032558194	40.0	35.0	41.0	31.0	41.0
35	36.56986234854709	40.0	35.0	41.0	30.0	41.0
36	36.42423118381389	40.0	35.0	41.0	30.0	41.0
37	36.364924439077804	40.0	35.0	41.0	30.0	41.0
38	36.26684902482055	40.0	35.0	41.0	30.0	41.0
39	36.18177552859895	39.0	35.0	41.0	29.0	41.0
40	36.071431521000854	39.0	35.0	41.0	29.0	41.0
41	35.99741485245934	39.0	35.0	41.0	28.0	41.0
42	35.943169472048936	39.0	35.0	41.0	28.0	41.0
43	35.85817144419399	39.0	35.0	41.0	27.0	41.0
44	35.68647307844011	39.0	35.0	40.0	27.0	41.0
45	35.58698512418818	39.0	35.0	40.0	27.0	41.0
46	35.57498138209634	39.0	35.0	40.0	27.0	41.0
47	35.53217729085651	38.0	35.0	40.0	26.0	41.0
48	35.45007838742612	38.0	35.0	40.0	26.0	41.0
49	35.3517210347425	38.0	35.0	40.0	26.0	41.0
50	35.25475343915043	38.0	34.0	40.0	26.0	41.0
51	34.89025546754077	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	2.0
14	7.0
15	23.0
16	58.0
17	186.0
18	380.0
19	823.0
20	1476.0
21	2548.0
22	4330.0
23	6790.0
24	10635.0
25	16330.0
26	21194.0
27	22884.0
28	22806.0
29	23759.0
30	27252.0
31	34129.0
32	44558.0
33	62108.0
34	93957.0
35	139789.0
36	148035.0
37	256683.0
38	323951.0
39	139834.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.21623538156379	22.95555482776837	28.294229802600384	16.533979988067454
2	33.57779863046274	23.992034527489707	29.937873871000352	12.4922929710472
3	28.995160057014214	24.414514987590437	33.846565690941375	12.743759264453972
4	25.813171650664053	26.449241827701446	33.55451735131664	14.183069170317864
5	22.36120584210594	31.52548623699061	32.44676988271077	13.666538038192687
6	20.56712341641024	39.76065136320077	29.5455095609877	10.126715659401293
7	86.1901432617428	3.4029113702349916	8.95581683115448	1.4511285368677211
8	32.70243677388396	63.32942226829431	3.243075065394051	0.7250658924276749
9	83.69377784675935	3.731554748028211	10.197485052279644	2.3771823529328002
10	42.07147851073858	22.189764495978107	22.854954071091203	12.883802922192114
11	37.648391455841754	22.796359292078243	23.378106484441414	16.177142767638593
12	33.79744005604594	21.848732914602557	27.041882095628388	17.31194493372311
13	24.454883444091468	28.323705183537644	27.852526267975353	19.368885104395535
14	19.262232639071826	31.87093200584951	30.180867772301973	18.685967582776694
15	19.42448962737138	24.419213961363045	39.031028890145116	17.125267521120463
16	22.32154935132803	21.382893741963688	37.770778363646464	18.524778543061824
17	22.36704396103554	21.410375618876206	29.646679890243366	26.575900529844894
18	23.97957512733507	24.30950004342991	32.24528358306718	19.465641246167845
19	26.85221442698863	25.830899597169793	27.82084379329642	19.496042182545164
20	29.147022345756184	24.601833169343895	27.23916779750556	19.01197668739436
21	25.64358141541634	25.485026648877017	30.272355367723176	18.59903656798347
22	24.21117757706673	21.976673155047624	30.380502961065442	23.431646306820205
23	22.345257809908002	25.978988467579217	29.53767793803335	22.138075784479433
24	22.29727132016956	24.452035581198977	34.002842167166705	19.247850931464754
25	21.95837563596338	24.68079016803815	32.25774298322182	21.103091212776654
26	20.94446524966502	28.853906057546762	28.41134816405399	21.790280528734225
27	20.18778807913072	28.286184589929103	31.647232375644506	19.87879495529567
28	18.3609552301714	27.04957132543811	35.13657638466653	19.452897059723956
29	19.823261628892137	25.725243883858457	34.18425103341824	20.267243453831156
30	21.835632745297108	25.75429208536184	33.028374681929314	19.381700487411734
31	26.941067749234282	24.74814212544551	28.268100660561796	20.04268946475841
32	27.093926789988622	25.749593111589235	28.77131803366459	18.385162064757555
33	25.657037567583345	25.74396858237657	28.15831554605635	20.440678303983734
34	21.376272460738647	27.123758153787442	29.606311433742334	21.89365795173157
35	21.642547641186365	25.905513604953	30.212479050408596	22.239459703452034
36	26.696080343907923	26.784577683292017	27.33514077698243	19.18420119581763
37	22.46123702620461	29.41600299595176	27.882001648912613	20.240758328931012
38	22.61907982702081	30.203152299435697	25.617238683660815	21.560529189882683
39	22.493845056323607	28.075585129029545	26.851004085259316	22.579565729387525
40	24.24727423922903	25.139438486873487	27.52694434279156	23.086342931105925
41	20.887507991815244	25.032714824977464	28.67562984047696	25.404147342730333
42	22.490214031135686	26.85036331610851	26.36387713749909	24.295545515256713
43	22.212760988834955	26.184604168416914	26.95067928649643	24.6519555562517
44	21.902130343836728	27.44834332695887	28.250728696917616	22.39879763228679
45	21.57590764950212	29.694666379981804	26.101802554817798	22.627623415698274
46	22.852533387632587	28.739706755557958	27.608820400950616	20.798939455858836
47	22.691557937634652	27.13579037450821	27.557630065458127	22.615021622399013
48	22.600853504508876	26.55660625874828	28.981703904847205	21.860836331895637
49	22.152884671520372	25.240466422984532	30.230206996914337	22.37644190858075
50	21.01167481392776	28.641455485767093	28.733441457194488	21.613428243110665
51	20.407358308141614	29.717235693404774	27.683363212161517	22.192042786292095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	830.0
1	1407.0
2	1984.0
3	13731.5
4	25479.0
5	17615.5
6	9752.0
7	9623.5
8	9495.0
9	9756.0
10	10017.0
11	9759.0
12	9501.0
13	9044.0
14	8587.0
15	8190.5
16	7794.0
17	7274.0
18	6754.0
19	6509.0
20	6264.0
21	6496.0
22	6728.0
23	6896.0
24	7064.0
25	7886.5
26	10142.5
27	11576.0
28	13397.5
29	15219.0
30	17667.0
31	20115.0
32	24347.0
33	28579.0
34	32515.5
35	36452.0
36	40728.5
37	45005.0
38	55034.5
39	65064.0
40	76635.0
41	88206.0
42	95149.0
43	102092.0
44	103637.0
45	105182.0
46	107835.5
47	110489.0
48	108932.5
49	107376.0
50	103191.0
51	99006.0
52	94028.0
53	89050.0
54	85174.5
55	81299.0
56	77277.5
57	73256.0
58	68525.0
59	63794.0
60	55924.5
61	48055.0
62	41208.5
63	34362.0
64	28597.5
65	22833.0
66	18645.0
67	14457.0
68	12222.0
69	9987.0
70	8181.5
71	6376.0
72	5148.0
73	3920.0
74	3008.0
75	1518.5
76	941.0
77	715.5
78	490.0
79	316.0
80	142.0
81	107.0
82	72.0
83	61.5
84	51.0
85	40.5
86	30.0
87	21.5
88	13.0
89	12.5
90	12.0
91	7.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1404562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.345784248911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25698889039509	18.77321255533397
2	6.074476948518508	3.200737182213037
3	3.1646251415051827	2.5012359362032495
4	2.1643042616460115	2.280811725052977
5	1.7455494163416587	2.299393415937493
6	1.464376036872813	2.3148081076035876
7	1.2129786155268774	2.2369811032249713
8	1.117465122211215	2.3552396012367707
9	0.9827151440273793	2.330136104641455
>10	10.527114489201466	54.82818316301392
>50	0.2602534030032331	4.1976311749712405
>100	0.026972902383546302	1.3102008039144886
>500	0.0016347212753157851	0.30793630029000635
>1k	0.0	0.0
>5k	5.449070917719283E-4	1.0634928263628751
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9530	0.6785033341354814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5286	0.3763450812424087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.11965723122226E-5	0.0	0.0	0.006051708646538921	0.0
2	7.11965723122226E-5	0.0	0.0	0.01801273279499232	0.0
3	7.11965723122226E-5	0.0	0.0	0.03139768838969017	0.0
4	7.11965723122226E-5	0.0	0.0	0.048057686310750254	0.0
5	7.11965723122226E-5	0.0	0.0	0.08372716903917378	0.0
6	1.423931446244452E-4	0.0	0.0	0.14260673434138188	0.0
7	1.423931446244452E-4	0.0	0.0	0.18646382288571098	0.0
8	1.423931446244452E-4	0.0	0.0	0.2659191975861514	0.0
9	1.423931446244452E-4	0.0	0.0	0.3868821739446176	0.0
10	1.423931446244452E-4	0.0	0.0	0.5662263395991063	0.0
11	1.423931446244452E-4	0.0	0.0	0.7385220445946851	0.0
12	1.423931446244452E-4	0.0	0.0	0.8631160461410746	0.0
13	1.423931446244452E-4	0.0	0.0	0.9234907394618393	0.0
14	1.423931446244452E-4	0.0	0.0	0.9521117615313528	0.0
15	1.423931446244452E-4	0.0	0.0	0.9869268853920297	0.0
16	1.423931446244452E-4	0.0	0.0	1.050007048460659	0.0
17	1.423931446244452E-4	0.0	0.0	1.1199932790435738	0.0
18	1.423931446244452E-4	0.0	0.0	1.2166070276712597	0.0
19	1.423931446244452E-4	0.0	0.0	1.2651630899881956	0.0
20	1.423931446244452E-4	0.0	0.0	1.326036159315146	0.0
21	1.423931446244452E-4	0.0	0.0	1.4064170894556451	0.0
22	1.423931446244452E-4	0.0	0.0	1.4916393865133757	0.0
23	1.423931446244452E-4	0.0	0.0	1.584479716808514	0.0
24	1.423931446244452E-4	0.0	0.0	1.6592361177363477	0.0
25	1.423931446244452E-4	0.0	0.0	1.7276560237283936	0.0
26	1.423931446244452E-4	0.0	0.0	1.789810631356964	0.0
27	1.423931446244452E-4	0.0	0.0	1.857660964770512	0.0
28	1.423931446244452E-4	0.0	0.0	1.933485314283029	0.0
29	1.423931446244452E-4	0.0	0.0	2.020701115365502	0.0
30	1.423931446244452E-4	0.0	0.0	2.1156061462576945	0.0
31	1.423931446244452E-4	0.0	0.0	2.204744254792597	0.0
32	1.423931446244452E-4	0.0	0.0	2.29196005587507	0.0
33	1.423931446244452E-4	0.0	0.0	2.3875770524903848	0.0
34	1.423931446244452E-4	0.0	0.0	2.4813429382255823	0.0
35	1.423931446244452E-4	0.0	0.0	2.6026618974456093	0.0
36	1.423931446244452E-4	0.0	0.0	2.714369319403487	0.0
37	1.423931446244452E-4	0.0	0.0	2.822588109318065	0.0
38	1.423931446244452E-4	0.0	0.0	2.9622045876223333	0.0
39	1.423931446244452E-4	0.0	0.0	3.1671083227369103	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCA	40	6.8175723E-9	45.0	39
CGATATT	25	3.891249E-5	45.0	10
CGTTTTT	8460	0.0	43.7234	1
GAGCGAT	805	0.0	41.925465	7
CGAGGTA	70	0.0	41.785713	6
CACGACG	275	0.0	41.72727	26
TATGCGA	155	0.0	40.64516	2
CGGTCTA	285	0.0	40.263157	31
GTTTTTA	6065	0.0	40.065952	2
ATCGCAA	45	1.9288564E-8	40.0	33
TATACGA	80	0.0	39.375	2
AGGGGAA	9270	0.0	39.126213	8
GAGGGGA	9265	0.0	39.001617	7
AGACTTA	1125	0.0	39.0	8
ACGGTCT	295	0.0	38.898304	30
TCGGACG	35	6.24928E-6	38.571426	1
ATCGGGT	35	6.24928E-6	38.571426	4
TCACGAC	300	0.0	38.250004	25
AACGTAG	65	9.094947E-12	38.076927	1
GAGACTT	1420	0.0	37.71127	7
>>END_MODULE