FastQCFastQC Report
Thu 26 May 2016
SRR1546952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546952_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences673754
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23140.3434487958513048No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.24504492737705452No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.14144628454896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.10033335609139242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG600.045.0000043
GATACGA302.164159E-645.0000042
AGATCGC207.031205E-445.0000048
GTCGACG302.164159E-645.0000041
CAAGTCG207.031205E-445.0000041
CCGATTT207.031205E-445.00000411
GTTTCGA207.031205E-445.0000042
ACGTTAG302.164159E-645.0000041
CGTGCCG207.031205E-445.00000441
ACCGCTC207.031205E-445.00000418
TAGCGGA406.8084773E-945.0000042
GGCAACG302.164159E-645.0000041
TCTTACC207.031205E-445.00000436
ATAGATC207.031205E-445.00000423
TCGATTG302.164159E-645.0000041
TAGCCGT207.031205E-445.00000444
ATCGTAG207.031205E-445.0000041
AGTATAA302.164159E-645.0000048
ATCCGAT406.8084773E-945.00000410
CCGTAGT207.031205E-445.00000423