##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546952_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	673754
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776338248084613	31.0	31.0	34.0	30.0	34.0
2	32.11162234287292	33.0	31.0	34.0	30.0	34.0
3	32.138969119292796	33.0	31.0	34.0	30.0	34.0
4	35.86241120646408	37.0	35.0	37.0	35.0	37.0
5	35.82977763397323	37.0	35.0	37.0	35.0	37.0
6	35.876285706652574	37.0	35.0	37.0	35.0	37.0
7	36.12384342059565	37.0	35.0	37.0	35.0	37.0
8	13.302236424570392	0.0	0.0	37.0	0.0	37.0
9	25.036090323768022	18.0	17.0	39.0	17.0	39.0
10	32.84367588170163	32.0	32.0	38.0	27.0	39.0
11	35.70335612107683	37.0	35.0	37.0	32.0	39.0
12	36.38872496489817	37.0	35.0	39.0	33.0	39.0
13	36.26615797457232	38.0	35.0	39.0	32.0	39.0
14	37.401714869225266	39.0	35.0	41.0	33.0	41.0
15	37.88171647218421	40.0	36.0	41.0	33.0	41.0
16	38.08951932010793	40.0	36.0	41.0	34.0	41.0
17	38.10563054171107	40.0	36.0	41.0	34.0	41.0
18	38.06010650771647	40.0	36.0	41.0	34.0	41.0
19	38.00638957245523	40.0	36.0	41.0	34.0	41.0
20	37.97320535388287	40.0	36.0	41.0	34.0	41.0
21	37.84955339782769	40.0	35.0	41.0	33.0	41.0
22	37.793221264734605	40.0	35.0	41.0	33.0	41.0
23	37.735672070221476	40.0	35.0	41.0	33.0	41.0
24	37.6408229116265	40.0	35.0	41.0	33.0	41.0
25	37.51704479676558	39.0	35.0	41.0	33.0	41.0
26	37.40396643285235	39.0	35.0	41.0	33.0	41.0
27	37.337955693027425	39.0	35.0	41.0	33.0	41.0
28	37.37332913793462	39.0	35.0	41.0	33.0	41.0
29	37.45231048721047	39.0	35.0	41.0	33.0	41.0
30	37.39744179626392	39.0	35.0	41.0	33.0	41.0
31	37.262680444197734	39.0	35.0	41.0	33.0	41.0
32	37.06179555149209	39.0	35.0	41.0	32.0	41.0
33	36.93617848650991	39.0	35.0	41.0	32.0	41.0
34	36.84894783556016	39.0	35.0	41.0	32.0	41.0
35	36.752335422127366	39.0	35.0	41.0	32.0	41.0
36	36.61226946333528	39.0	35.0	41.0	31.0	41.0
37	36.49723934848624	39.0	35.0	41.0	31.0	41.0
38	36.453279683682766	39.0	35.0	41.0	31.0	41.0
39	36.45702140543878	39.0	35.0	41.0	31.0	41.0
40	36.311821525363854	39.0	35.0	41.0	30.0	41.0
41	36.30362862409722	39.0	35.0	41.0	31.0	41.0
42	36.25527121174791	39.0	35.0	41.0	30.0	41.0
43	36.132146154234334	39.0	35.0	41.0	30.0	41.0
44	35.94549197481573	38.0	35.0	40.0	30.0	41.0
45	35.84979829433295	38.0	35.0	40.0	30.0	41.0
46	35.873560676448676	38.0	35.0	40.0	30.0	41.0
47	35.84457235133298	38.0	35.0	40.0	30.0	41.0
48	35.826684516900826	38.0	35.0	40.0	30.0	41.0
49	35.784738940325404	38.0	35.0	40.0	29.0	41.0
50	35.63419734799348	38.0	35.0	40.0	29.0	41.0
51	35.17588170162997	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	6.0
14	7.0
15	13.0
16	29.0
17	65.0
18	148.0
19	360.0
20	629.0
21	1144.0
22	1894.0
23	2923.0
24	4236.0
25	6077.0
26	8068.0
27	8654.0
28	9367.0
29	10565.0
30	12714.0
31	17144.0
32	22827.0
33	32558.0
34	54864.0
35	92503.0
36	64139.0
37	110543.0
38	164305.0
39	47963.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.87617439005928	24.05299263529419	30.220970858800094	13.849862115846436
2	29.394111203792484	25.692463421367446	32.136952062622264	12.776473312217812
3	28.303653855858368	24.674584492262756	33.57813089050306	13.443630761375813
4	25.85038456172431	27.294977098466205	32.54822976932233	14.30640857048715
5	23.343534880683453	32.60225542260232	30.13043336291881	13.92377633379542
6	20.796017537558217	41.37474508500135	27.024106721444323	10.805130655996106
7	88.17862305826756	4.45889746109114	6.015251857502887	1.3472276231384155
8	33.87482671716977	63.439029675519556	1.9493761818111657	0.7367674254995147
9	88.34767585795409	3.6183829706391357	5.94341554929544	2.0905256221113344
10	61.63466190924284	17.077449632952085	11.026279621345477	10.2616088364596
11	58.564698688245265	16.91759900497808	14.336241417490655	10.181460889286
12	51.299584121207445	20.948892325685637	16.668249835993553	11.083273717113368
13	21.191859343321156	48.58271713414688	17.501343220225777	12.72408030230618
14	13.051054242349583	50.82938283112234	24.11503308329153	12.004529843236552
15	11.54991287621298	22.100944855243903	54.87818996250857	11.470952306034548
16	13.043633136129804	14.823659674005645	53.978603466547135	18.154103723317412
17	13.999026350863966	15.672782647672593	27.011639262995104	43.31655173846835
18	22.41990400056994	22.73455890428851	34.845804254965465	19.999732840176087
19	31.303116567768058	25.47457974275477	22.502871968107055	20.719431721370114
20	35.044541479531105	23.136337595027264	21.625548790804952	20.19357213463668
21	21.471635047806767	29.08257316468622	26.92421863172612	22.521573155780892
22	22.591925242744384	23.999412248387394	24.798962232506227	28.609700276361995
23	19.178810070144294	31.78816007029272	22.36587834728996	26.667151512273023
24	21.642172068737253	22.81589422845727	39.708558316536894	15.833375386268578
25	16.324207351644667	23.875628196641504	37.7634863763332	22.036678075380628
26	14.496240467589061	39.30010063020034	25.654318935397786	20.549339966812813
27	16.790549666495487	36.91584762391021	29.45986220489971	16.833740504694592
28	12.191541719974946	29.595965292970433	43.19825930532509	15.014233681729532
29	12.895062589609857	25.159479572663017	41.243539927035684	20.70191791069144
30	16.91418529611698	31.70073943902374	33.09368107647598	18.291394188383297
31	33.81278626917243	26.020624738406006	21.755566571775457	18.411022420646113
32	32.64885996966252	28.379497561424493	23.78805914324813	15.183583325664857
33	28.428922128848217	30.27187964746777	22.97708065555084	18.322117568133176
34	18.10378862314732	29.69169756320556	27.242287244305786	24.962226569341333
35	19.129830769093765	26.520955719743405	31.008943917216076	23.340269593946754
36	32.64084517494516	23.396521579092667	26.664034647660717	17.298598598301457
37	19.180145869263857	32.756762854098085	30.251100550052396	17.811990726585666
38	18.89977647628066	34.72276231384155	21.83437871982949	24.543082490048295
39	19.372946208853676	31.702965770889673	28.03204136821451	20.89204665204214
40	23.992733252789595	25.85587618032694	24.090098166393076	26.061292400490387
41	16.182167378598123	22.46739908037652	28.17022236602677	33.18021117499859
42	23.776779061794066	25.16912701074873	22.437120966999824	28.616972960457375
43	23.3653529329696	26.225447270071868	24.470800915467663	25.93839888149087
44	19.47431851981584	31.971906660294408	27.552637906416884	21.00113691347287
45	16.271220653235456	41.654075523113775	19.72144135693443	22.353262466716338
46	24.08223179380011	33.107781178293564	23.799784491075375	19.01020253683095
47	22.109404916334448	28.47804985202314	25.238143298592664	24.17440193304975
48	24.94352538166749	23.178341056231204	30.153587214324517	21.724546347776787
49	21.254790324064867	21.919127752859353	31.49042528875524	25.335656634320536
50	19.339996497237863	33.91920493236404	26.23168099929648	20.50911757110162
51	17.002793304381125	37.303377790706996	21.954749062714285	23.739079842197597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	813.0
1	882.5
2	952.0
3	2625.0
4	4298.0
5	3263.5
6	2229.0
7	2561.5
8	2894.0
9	3146.0
10	3398.0
11	3414.5
12	3431.0
13	3321.0
14	3211.0
15	3025.0
16	2839.0
17	2590.5
18	2342.0
19	2109.0
20	1876.0
21	1845.0
22	1814.0
23	1982.0
24	2150.0
25	2304.0
26	2856.0
27	3254.0
28	4066.5
29	4879.0
30	6057.0
31	7235.0
32	8457.5
33	9680.0
34	10420.5
35	11161.0
36	14685.5
37	18210.0
38	30127.5
39	42045.0
40	56457.5
41	70870.0
42	76675.5
43	82481.0
44	78090.5
45	73700.0
46	69804.5
47	65909.0
48	60637.5
49	55366.0
50	50749.5
51	46133.0
52	41440.0
53	36747.0
54	32582.0
55	28417.0
56	26472.0
57	24527.0
58	22415.0
59	20303.0
60	17492.0
61	14681.0
62	12267.0
63	9853.0
64	7568.5
65	5284.0
66	4238.5
67	3193.0
68	2558.0
69	1923.0
70	1562.0
71	1201.0
72	1079.5
73	958.0
74	761.5
75	405.5
76	246.0
77	195.0
78	144.0
79	99.5
80	55.0
81	37.0
82	19.0
83	13.0
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	673754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.242842087922206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85525268980207	17.09479249206626
2	5.1601877370413	2.2955448195808454
3	1.9048334540766283	1.2710672916845358
4	1.2273355773444592	1.0919772574304654
5	0.9058421731159644	1.0074252206599343
6	0.7834159941852114	1.0455238948688543
7	0.691744185437384	1.0770449677345477
8	0.5976482703243522	1.0634716880755528
9	0.5745458436225594	1.1501579224771772
>10	10.129747672925363	54.324896462075934
>50	1.08366466030701	15.20625168199802
>100	0.08310106238594263	2.537977680227606
>500	0.0013403397159023006	0.24282659703340595
>1k	0.0013403397159023006	0.5910420240868353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2314	0.3434487958513048	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1651	0.24504492737705452	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.14144628454896	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	676	0.10033335609139242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.968442487911018E-4	0.0	0.0	0.012912724822412928	0.0
2	2.968442487911018E-4	0.0	0.0	0.038292908094052135	0.0
3	2.968442487911018E-4	0.0	0.0	0.060110960380198114	0.0
4	2.968442487911018E-4	0.0	0.0	0.10063020034018351	0.0
5	2.968442487911018E-4	0.0	0.0	0.18938663072872294	0.0
6	2.968442487911018E-4	0.0	0.0	0.27131564339506703	0.0
7	2.968442487911018E-4	0.0	0.0	0.32623182942142087	0.0
8	2.968442487911018E-4	0.0	0.0	0.43517366872775526	0.0
9	2.968442487911018E-4	0.0	0.0	0.5263048531066234	0.0
10	2.968442487911018E-4	0.0	0.0	0.7192536148208396	0.0
11	2.968442487911018E-4	0.0	0.0	0.9291224987161486	0.0
12	2.968442487911018E-4	0.0	0.0	1.1364682064967333	0.0
13	2.968442487911018E-4	0.0	0.0	1.2105308465701132	0.0
14	2.968442487911018E-4	0.0	0.0	1.244964779429881	0.0
15	2.968442487911018E-4	0.0	0.0	1.2951314574755772	0.0
16	2.968442487911018E-4	0.0	0.0	1.3970974569353205	0.0
17	2.968442487911018E-4	0.0	0.0	1.5159835785761568	0.0
18	2.968442487911018E-4	0.0	0.0	1.6894890419945559	0.0
19	2.968442487911018E-4	0.0	0.0	1.7653327475606824	0.0
20	2.968442487911018E-4	0.0	0.0	1.8389501212608756	0.0
21	2.968442487911018E-4	0.0	0.0	1.9570941322797342	0.0
22	2.968442487911018E-4	0.0	0.0	2.071824434437495	0.0
23	2.968442487911018E-4	0.0	0.0	2.2263318659332634	0.0
24	2.968442487911018E-4	0.0	0.0	2.316720939690154	0.0
25	2.968442487911018E-4	0.0	0.0	2.3925646452562805	0.0
26	2.968442487911018E-4	0.0	0.0	2.4774621004105355	0.0
27	2.968442487911018E-4	0.0	0.0	2.543064679393369	0.0
28	2.968442487911018E-4	0.0	0.0	2.6254389584328996	0.0
29	2.968442487911018E-4	0.0	0.0	2.710930102084737	0.0
30	2.968442487911018E-4	0.0	0.0	2.829964645849969	0.0
31	2.968442487911018E-4	0.0	0.0	2.9512255214811343	0.0
32	2.968442487911018E-4	0.0	0.0	3.0479967465870335	0.0
33	2.968442487911018E-4	0.0	0.0	3.146994303558866	0.0
34	2.968442487911018E-4	0.0	0.0	3.247624503899049	0.0
35	2.968442487911018E-4	0.0	0.0	3.4058424885047063	0.0
36	2.968442487911018E-4	0.0	0.0	3.5251738765187293	0.0
37	2.968442487911018E-4	0.0	0.0	3.6440599981595656	0.0
38	2.968442487911018E-4	0.0	0.0	3.749142862231616	0.0
39	2.968442487911018E-4	0.0	0.0	3.868622672370034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	60	0.0	45.000004	3
GATACGA	30	2.164159E-6	45.000004	2
AGATCGC	20	7.031205E-4	45.000004	8
GTCGACG	30	2.164159E-6	45.000004	1
CAAGTCG	20	7.031205E-4	45.000004	1
CCGATTT	20	7.031205E-4	45.000004	11
GTTTCGA	20	7.031205E-4	45.000004	2
ACGTTAG	30	2.164159E-6	45.000004	1
CGTGCCG	20	7.031205E-4	45.000004	41
ACCGCTC	20	7.031205E-4	45.000004	18
TAGCGGA	40	6.8084773E-9	45.000004	2
GGCAACG	30	2.164159E-6	45.000004	1
TCTTACC	20	7.031205E-4	45.000004	36
ATAGATC	20	7.031205E-4	45.000004	23
TCGATTG	30	2.164159E-6	45.000004	1
TAGCCGT	20	7.031205E-4	45.000004	44
ATCGTAG	20	7.031205E-4	45.000004	1
AGTATAA	30	2.164159E-6	45.000004	8
ATCCGAT	40	6.8084773E-9	45.000004	10
CCGTAGT	20	7.031205E-4	45.000004	23
>>END_MODULE