FastQCFastQC Report
Thu 26 May 2016
SRR1546951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546951_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1733638
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96160.5546717365447689No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG59680.34424718424492307No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.30848423950098003No Hit
GAATCTGACTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC37740.21769250558651806No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC25990.14991595707985172No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC25760.14858926719418933No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.034281E-445.023
CGTTTTT81050.043.056761
CGGTCTA2900.041.8965531
ATTGCGA1950.041.538462
TATGCGA1250.041.42
CACGACG2950.040.4237326
GAGGGGA129450.039.9768267
AGACTTA13600.039.7058838
AGGGGAA129800.039.661028
TAACGAG2800.039.3750043
TCGACGA403.4595723E-739.3752
TAGTCGA403.4595723E-739.3752
GTTTTTA61000.039.0614782
CGATGAA9600.038.90624619
GTTAACG700.038.571431
CGACGGT3100.038.46774328
TTACGAG2650.038.2075463
CTATGCG659.094947E-1238.0769271
ACGTCGT659.094947E-1238.07692737
TTAACGA950.037.8947372