##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546951_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1733638
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.667985473322574	31.0	31.0	34.0	30.0	34.0
2	32.00840313837145	31.0	31.0	34.0	30.0	34.0
3	32.02856247959493	33.0	31.0	34.0	30.0	34.0
4	35.79300753675219	37.0	35.0	37.0	35.0	37.0
5	35.77098160054175	37.0	35.0	37.0	35.0	37.0
6	35.82105318411341	37.0	35.0	37.0	35.0	37.0
7	36.018128928876735	37.0	35.0	37.0	35.0	37.0
8	13.362477056917303	0.0	0.0	35.0	0.0	37.0
9	24.95692526352099	18.0	17.0	38.0	17.0	39.0
10	32.54140022311463	32.0	32.0	37.0	27.0	39.0
11	35.46856321792669	37.0	34.0	37.0	32.0	39.0
12	36.52617559144412	37.0	35.0	39.0	33.0	39.0
13	36.755664100579246	39.0	35.0	39.0	33.0	39.0
14	37.939958630348436	40.0	37.0	41.0	33.0	41.0
15	38.20713609184847	40.0	37.0	41.0	33.0	41.0
16	38.313403374868344	40.0	37.0	41.0	34.0	41.0
17	38.25325760049099	40.0	37.0	41.0	33.0	41.0
18	38.2074723788934	40.0	37.0	41.0	33.0	41.0
19	38.18224681277176	40.0	37.0	41.0	34.0	41.0
20	38.10971263897076	40.0	37.0	41.0	34.0	41.0
21	38.08892686939257	40.0	37.0	41.0	33.0	41.0
22	38.029151991361516	40.0	36.0	41.0	33.0	41.0
23	37.895197844071255	40.0	36.0	41.0	33.0	41.0
24	37.81112896694697	40.0	36.0	41.0	33.0	41.0
25	37.73443475512189	40.0	35.0	41.0	33.0	41.0
26	37.64012960029718	40.0	35.0	41.0	33.0	41.0
27	37.5656434618992	40.0	35.0	41.0	33.0	41.0
28	37.506094121148706	40.0	35.0	41.0	33.0	41.0
29	37.50930701795877	40.0	35.0	41.0	33.0	41.0
30	37.418451833658466	40.0	35.0	41.0	33.0	41.0
31	37.35685823684068	40.0	35.0	41.0	33.0	41.0
32	37.15242455460713	40.0	35.0	41.0	32.0	41.0
33	36.9879801896359	40.0	35.0	41.0	31.0	41.0
34	36.7926856702495	40.0	35.0	41.0	31.0	41.0
35	36.62359616021338	40.0	35.0	41.0	30.0	41.0
36	36.495154120987195	40.0	35.0	41.0	30.0	41.0
37	36.43183698096142	40.0	35.0	41.0	30.0	41.0
38	36.35592205523875	39.0	35.0	41.0	30.0	41.0
39	36.26727379072217	39.0	35.0	41.0	30.0	41.0
40	36.16437110861668	39.0	35.0	41.0	29.0	41.0
41	36.08110343681899	39.0	35.0	41.0	29.0	41.0
42	36.037367085862215	39.0	35.0	41.0	29.0	41.0
43	35.92689881047831	39.0	35.0	41.0	29.0	41.0
44	35.71500220922707	39.0	35.0	40.0	27.0	41.0
45	35.657590569657565	38.0	35.0	40.0	27.0	41.0
46	35.636003594752765	38.0	35.0	40.0	27.0	41.0
47	35.56586899917976	38.0	35.0	40.0	27.0	41.0
48	35.507337171889404	38.0	35.0	40.0	27.0	41.0
49	35.418268404361235	38.0	35.0	40.0	27.0	41.0
50	35.295824157061624	38.0	34.0	40.0	27.0	41.0
51	34.93718008027051	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	5.0
13	7.0
14	17.0
15	32.0
16	100.0
17	236.0
18	556.0
19	1094.0
20	2098.0
21	3664.0
22	5882.0
23	9011.0
24	13373.0
25	18633.0
26	23287.0
27	25307.0
28	26028.0
29	28959.0
30	34550.0
31	44862.0
32	59056.0
33	81474.0
34	121866.0
35	182335.0
36	187247.0
37	308157.0
38	391894.0
39	163868.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.74310207782709	22.554247195781358	26.451715986843848	15.250934739547702
2	32.697887332880335	26.266613906709473	28.20658061256156	12.828918147848627
3	29.111556161090146	26.1759375371329	31.191863583977742	13.520642717799216
4	25.810463314717374	26.336005555946514	32.905600823239915	14.947930306096197
5	23.959500195542553	30.260354237735903	30.436111806501703	15.344033760219839
6	20.892193179891073	40.722746040407515	27.811688483985698	10.573372295715714
7	83.50416869034942	4.267557586993363	10.107473417172443	2.120800305484767
8	31.35827664137496	63.867139506632874	3.352199248055246	1.4223846039369235
9	78.95679490181918	5.4333142213080245	10.359140720265707	5.250750156607089
10	44.15120111580388	25.99245055772889	17.20313006521546	12.653218261251773
11	37.99034169763238	22.681724789142834	23.423978939086474	15.903954574138316
12	33.008505812632166	21.508527155034674	28.115269739126624	17.36769729320654
13	23.589065306598034	31.082844284677652	28.786286410427092	16.541803998297222
14	17.81975245120377	34.78286701145222	30.11816769129426	17.279212846049752
15	16.693277373938503	24.74432378616528	40.93490105777561	17.627497782120606
16	19.50955159035508	21.844006649600438	38.87732040945111	19.769121350593377
17	20.40656699956969	22.372202270600898	29.36547306877214	27.855757661057268
18	22.91297260443068	24.182268731995954	32.17240277382014	20.732355889753222
19	25.979587434054864	25.98258690683984	26.97691213505934	21.060913524045965
20	28.261782448238904	25.00245149218003	26.972643654557643	19.763122405023424
21	24.068807905687347	27.412354828401313	29.59095266716581	18.92788459874553
22	23.218457371146688	22.52332955322853	29.38854593634888	24.869667139275904
23	21.08017936847254	27.79259568606595	28.66267352238472	22.464551423076788
24	21.21844352742614	25.125083783350387	34.08595104629686	19.570521642926607
25	20.344962443139803	26.773351760863573	31.91929341650333	20.9623923794933
26	19.736588607310175	29.533789637744444	28.740371403949382	21.989250350996
27	20.63175818711865	28.370224925849573	30.334187413981468	20.663829473050313
28	17.826501264969966	27.259670127212253	35.06787460819387	19.845953999623912
29	18.895928677151748	26.25726939534089	34.05814824086689	20.788653686640462
30	20.666079077639047	27.5026274227953	31.938674625267787	19.892618874297863
31	26.43608411906061	25.669661140330334	26.30174234759506	21.592512393013997
32	26.70949759984495	26.207489683544082	27.27166801835216	19.811344698258807
33	24.534591419892735	25.512015772612273	27.344405233387825	22.608987574107168
34	20.058916567357198	27.89273193134899	28.65828967754514	23.390061823748674
35	20.79471031437936	25.432587425979357	30.173946348661023	23.598755910980262
36	25.861050576879375	25.938575411937208	27.723434765504678	20.47693924567874
37	20.807631120222332	28.990712017156984	28.681131816446108	21.520525046174576
38	20.9068444508023	29.84435043532733	25.235141361691426	24.013663752178942
39	21.256744487603523	28.310004741474287	27.17689621478071	23.256354556141478
40	22.42798092796766	25.50740119909693	27.415123572510524	24.649494300424887
41	19.8067878069124	24.92492665712219	28.550193292948123	26.71809224301728
42	21.93837467798929	26.286456572825468	26.53177883733513	25.243389911850112
43	22.067640418587963	25.75520379687109	27.419276688674337	24.75787909586661
44	20.604416839040216	27.92030400810319	28.616008647710768	22.859270505145826
45	20.117983108353645	29.99276665601469	26.224102148199336	23.665148087432325
46	23.342935491723185	28.228038379407927	27.202737826466656	21.22628830240223
47	21.075564794957195	27.498012849279952	28.898305182512154	22.528117173250703
48	22.042317946422493	25.582907158241802	29.780611638646594	22.59416325668911
49	21.452113993809547	24.81360007106443	31.196074382310496	22.538211552815525
50	20.262246212877198	27.8774461565794	29.504487095921988	22.355820534621415
51	19.884831781490714	28.89519034538929	27.27835914994941	23.94161872317058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1306.0
1	2241.0
2	3176.0
3	14184.5
4	25193.0
5	17390.5
6	9588.0
7	9653.5
8	9719.0
9	9830.5
10	9942.0
11	9849.0
12	9756.0
13	9291.5
14	8827.0
15	8503.0
16	8179.0
17	7806.5
18	7434.0
19	7699.0
20	7964.0
21	8574.0
22	9184.0
23	9804.5
24	10425.0
25	11900.5
26	16099.5
27	18823.0
28	22060.5
29	25298.0
30	27772.0
31	30246.0
32	33332.0
33	36418.0
34	41370.0
35	46322.0
36	51802.5
37	57283.0
38	69082.5
39	80882.0
40	97056.5
41	113231.0
42	122974.5
43	132718.0
44	135849.0
45	138980.0
46	148567.5
47	158155.0
48	146911.0
49	135667.0
50	127287.5
51	118908.0
52	110075.0
53	101242.0
54	94917.0
55	88592.0
56	82458.5
57	76325.0
58	70554.5
59	64784.0
60	59166.5
61	53549.0
62	47494.0
63	41439.0
64	34986.0
65	28533.0
66	23933.5
67	19334.0
68	16153.5
69	12973.0
70	10876.5
71	8780.0
72	7095.5
73	5411.0
74	4258.0
75	2289.0
76	1473.0
77	1046.5
78	620.0
79	442.0
80	264.0
81	185.5
82	107.0
83	83.0
84	59.0
85	40.5
86	22.0
87	16.5
88	11.0
89	9.5
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1733638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.758213921905277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1931950623776	18.61649208825896
2	6.189663713615841	3.064900366527113
3	2.5335455678818395	1.881781894515407
4	1.5966415860428622	1.5811997577543726
5	1.2358914243546748	1.5299232134210627
6	0.9553700105783212	1.4191953057882594
7	0.8232797073538252	1.4268054578560707
8	0.7514247159129073	1.4883147090222928
9	0.6506539874820445	1.4498127550099023
>10	9.314825875711264	51.7892135481674
>50	0.6990024336659328	10.942661385274269
>100	0.05012240360359672	2.102926766512196
>500	0.004492100628450327	0.7913338703524567
>1k	0.0011821317443290334	0.690185491924389
>5k	7.092790465974201E-4	1.2252533896159201
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9616	0.5546717365447689	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	5968	0.34424718424492307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5348	0.30848423950098003	No Hit
GAATCTGACTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	3774	0.21769250558651806	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	2599	0.14991595707985172	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	2576	0.14858926719418933	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019958030453877916	0.0
2	0.0	0.0	0.0	0.08087040085646484	0.0
3	0.0	0.0	0.0	0.11305705112601362	0.0
4	0.0	0.0	0.0	0.2114051491718571	0.0
5	0.0	0.0	0.0	0.48441485477360324	0.0
6	0.0	0.0	0.0	0.6415410829711854	0.0
7	0.0	0.0	0.0	0.7740947071995422	0.0
8	0.0	0.0	0.0	0.9857882672161086	0.0
9	0.0	0.0	0.0	1.197770238077384	0.0
10	0.0	0.0	0.0	1.9165477452616981	0.0
11	0.0	0.0	0.0	2.2444708756960794	0.0
12	0.0	0.0	0.0	2.697910405747913	0.0
13	0.0	0.0	0.0	2.838078076276593	0.0
14	0.0	0.0	0.0	2.898586671496587	0.0
15	0.0	0.0	0.0	3.0087019320065664	0.0
16	0.0	0.0	0.0	3.154349408584722	0.0
17	0.0	0.0	0.0	3.297228141053669	0.0
18	0.0	0.0	0.0	3.4959432130583203	0.0
19	0.0	0.0	0.0	3.624747496305457	0.0
20	0.0	0.0	0.0	3.735439578504855	0.0
21	0.0	0.0	0.0	3.873184597937978	0.0
22	0.0	0.0	0.0	4.017620748968354	0.0
23	0.0	0.0	0.0	4.171113000522601	0.0
24	0.0	0.0	0.0	4.295706485436983	0.0
25	0.0	0.0	0.0	4.400168893390662	0.0
26	0.0	0.0	0.0	4.507919184974026	0.0
27	0.0	0.0	0.0	4.6261676313048055	0.0
28	0.0	0.0	0.0	4.743608527270399	0.0
29	0.0	0.0	0.0	4.873335725220605	0.0
30	0.0	0.0	0.0	5.025616651227073	0.0
31	0.0	0.0	0.0	5.158631732806964	0.0
32	0.0	0.0	0.0	5.28801283774352	0.0
33	0.0	0.0	0.0	5.4221815627022485	0.0
34	0.0	0.0	0.0	5.554158365241187	0.0
35	0.0	0.0	0.0	5.720859833483114	0.0
36	0.0	0.0	0.0	5.858316442071528	0.0
37	0.0	0.0	0.0	5.992658213537082	0.0
38	0.0	0.0	0.0	6.159128953103243	0.0
39	0.0	0.0	0.0	6.358997668486731	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	7.034281E-4	45.0	23
CGTTTTT	8105	0.0	43.05676	1
CGGTCTA	290	0.0	41.89655	31
ATTGCGA	195	0.0	41.53846	2
TATGCGA	125	0.0	41.4	2
CACGACG	295	0.0	40.42373	26
GAGGGGA	12945	0.0	39.976826	7
AGACTTA	1360	0.0	39.705883	8
AGGGGAA	12980	0.0	39.66102	8
TAACGAG	280	0.0	39.375004	3
TCGACGA	40	3.4595723E-7	39.375	2
TAGTCGA	40	3.4595723E-7	39.375	2
GTTTTTA	6100	0.0	39.061478	2
CGATGAA	960	0.0	38.906246	19
GTTAACG	70	0.0	38.57143	1
CGACGGT	310	0.0	38.467743	28
TTACGAG	265	0.0	38.207546	3
CTATGCG	65	9.094947E-12	38.076927	1
ACGTCGT	65	9.094947E-12	38.076927	37
TTAACGA	95	0.0	37.894737	2
>>END_MODULE