FastQCFastQC Report
Thu 26 May 2016
SRR1546950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546950_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences972221
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.5527549806062613No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.31268610737682073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG453.8380676E-1045.01
TATGCGA1050.045.02
GATTCGC207.032751E-445.09
CAATCGA207.032751E-445.02
CGTTTTT46900.043.1289981
CGTTAGA800.042.18752
TATAGCG650.041.538461
CGTAAGA1150.041.0869562
CGGTCTA1050.040.71428731
GACCGAT2600.040.6730779
CTATGCG501.0804797E-940.5000041
GTATGCG501.0804797E-940.5000041
TAAGCGA950.040.2631572
ACGAGGT1400.040.178575
AGGGGAA69400.039.4884728
GAGCGAT6800.039.3750047
CGATAAG403.4574987E-739.37510
GTTTTTA34250.039.284672
GAGGGGA69550.039.20927
TAGAGTA4150.039.0361445