##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546950_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	972221
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69338658597171	31.0	31.0	33.0	30.0	34.0
2	32.05154177908109	33.0	31.0	34.0	30.0	34.0
3	32.072474262539075	33.0	31.0	34.0	30.0	34.0
4	35.834590077770386	37.0	35.0	37.0	35.0	37.0
5	35.826516810478275	37.0	35.0	37.0	35.0	37.0
6	35.872698697106934	37.0	35.0	37.0	35.0	37.0
7	36.11176882622367	37.0	35.0	37.0	35.0	37.0
8	13.320892060550019	0.0	0.0	36.0	0.0	37.0
9	24.98140237662013	18.0	17.0	39.0	17.0	39.0
10	32.525357917592814	32.0	32.0	37.0	27.0	39.0
11	35.477352371528696	37.0	34.0	37.0	32.0	39.0
12	36.60165127064731	37.0	35.0	39.0	33.0	39.0
13	36.8559473617624	39.0	35.0	39.0	33.0	39.0
14	38.025462317724056	40.0	37.0	41.0	33.0	41.0
15	38.31209159234372	40.0	37.0	41.0	33.0	41.0
16	38.38898871758582	40.0	37.0	41.0	34.0	41.0
17	38.374085727422056	40.0	37.0	41.0	34.0	41.0
18	38.315199939108496	40.0	37.0	41.0	34.0	41.0
19	38.28967693559386	40.0	37.0	41.0	34.0	41.0
20	38.258564667909866	40.0	37.0	41.0	34.0	41.0
21	38.18216022900143	40.0	37.0	41.0	34.0	41.0
22	38.1298418775155	40.0	37.0	41.0	33.0	41.0
23	38.00730698061449	40.0	36.0	41.0	33.0	41.0
24	37.89911964460755	40.0	36.0	41.0	33.0	41.0
25	37.795962029209406	40.0	36.0	41.0	33.0	41.0
26	37.71852798900662	40.0	35.0	41.0	33.0	41.0
27	37.668182440000784	40.0	35.0	41.0	33.0	41.0
28	37.603845216262556	40.0	35.0	41.0	33.0	41.0
29	37.608559165045804	40.0	36.0	41.0	33.0	41.0
30	37.51544967656531	40.0	36.0	41.0	33.0	41.0
31	37.43741083560219	40.0	35.0	41.0	33.0	41.0
32	37.28386241399846	40.0	35.0	41.0	32.0	41.0
33	37.12786290359908	40.0	35.0	41.0	32.0	41.0
34	36.95298702661226	40.0	35.0	41.0	31.0	41.0
35	36.804002382174424	40.0	35.0	41.0	31.0	41.0
36	36.68531434725232	40.0	35.0	41.0	31.0	41.0
37	36.618849006553035	40.0	35.0	41.0	30.0	41.0
38	36.522845114433856	40.0	35.0	41.0	30.0	41.0
39	36.4406868397206	39.0	35.0	41.0	30.0	41.0
40	36.339595626920214	39.0	35.0	41.0	30.0	41.0
41	36.282152926135105	39.0	35.0	41.0	30.0	41.0
42	36.22375159557343	39.0	35.0	41.0	30.0	41.0
43	36.14153777793321	39.0	35.0	41.0	29.0	41.0
44	35.97042853425302	39.0	35.0	40.0	29.0	41.0
45	35.86592451716225	39.0	35.0	40.0	28.0	41.0
46	35.844149632645255	39.0	35.0	40.0	29.0	41.0
47	35.80614284200814	39.0	35.0	40.0	28.0	41.0
48	35.72528365464231	38.0	35.0	40.0	28.0	41.0
49	35.63124330784873	38.0	35.0	40.0	28.0	41.0
50	35.53005335206707	38.0	35.0	40.0	27.0	41.0
51	35.156554939669064	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	4.0
14	8.0
15	18.0
16	46.0
17	101.0
18	293.0
19	535.0
20	984.0
21	1659.0
22	2748.0
23	4181.0
24	6476.0
25	9689.0
26	12632.0
27	13763.0
28	14421.0
29	15678.0
30	18971.0
31	23903.0
32	32085.0
33	43869.0
34	66364.0
35	100267.0
36	104136.0
37	177527.0
38	225323.0
39	96526.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13485308381531	22.85941159468886	28.451041481309293	15.554693840186543
2	33.36309337074595	24.07940169981928	29.771317426799047	12.786187502635718
3	29.686460177264223	24.62526524319059	32.73535543873255	12.952919140812636
4	26.17871862467484	27.25100568697858	31.9632058966017	14.60706979174488
5	22.942108841508258	31.326930810998732	31.097353379529963	14.633606967963045
6	20.680894570267462	40.58573102206186	28.18309828732356	10.550276120347123
7	86.34456569031114	3.753261861243483	8.076455867544519	1.8257165809008447
8	32.81640696919733	63.295073856664274	2.955706572888263	0.9328126012501273
9	83.04253868204863	4.505971378935448	9.708286490417303	2.743203448598621
10	43.185551433264656	24.839002654746196	19.593590346227863	12.381855565761283
11	37.78451607196306	23.239880644421383	23.008863211142323	15.966740072473234
12	33.55636218514103	22.928017395221868	26.76058221330335	16.755038206333744
13	23.474498082226162	30.749695799617577	27.925543677826337	17.850262440329924
14	18.826686524977347	33.42871631038622	29.656117281976012	18.088479882660423
15	18.687006349379413	24.725756798094263	39.88753585861651	16.699700993909822
16	21.260701013452703	22.07142203264484	38.63370571094432	18.034171242958134
17	21.695170131071023	21.4376155215738	29.29477968486589	27.572434662489293
18	23.83316139026003	25.021780027380608	31.78793710483522	19.357121477524142
19	26.770147939614553	25.85965536642389	27.432651629619194	19.937545064342366
20	29.0137736173154	25.03278575550209	26.462398981301575	19.491041645880927
21	25.29260322498691	26.70431928542996	28.87399058444531	19.129086905137825
22	24.038464505498236	22.96412029775123	29.695305902670277	23.302109294080257
23	22.13251925230992	27.42442304784612	28.529830151786477	21.913227548057492
24	22.23784509900527	24.486922212130782	34.23552875323615	19.039703935627806
25	21.385878313675594	25.34331186016348	32.04168599526239	21.22912383089853
26	19.842402087591196	30.445443988558157	28.186492577304957	21.525661346545693
27	20.121145295154086	29.252711060551047	31.00221040277879	19.623933241516074
28	18.177657137626117	27.560194647101845	35.2129814106052	19.04916680466684
29	19.466664472378195	25.937106892362948	34.269677367594404	20.32655126766445
30	21.46908984685581	26.545199085393133	32.29903489021529	19.686676177535766
31	27.03140541090966	25.403894793467742	27.67662907919084	19.88807071643176
32	26.68837640824463	26.836902309248618	27.553920353499873	18.92080092900688
33	24.88148270814969	27.2503885433456	27.336994366507206	20.53113438199751
34	20.691180297483804	27.64926904479537	29.630402963935154	22.02914769378567
35	20.877249102827445	27.325474352024898	29.766894564096024	22.030381981051633
36	26.10568996143881	27.040251136315714	27.186719891876436	19.66733901036904
37	21.64651864133772	29.881580422558248	27.833074990151417	20.638825945952618
38	21.60784430700427	31.00210754550663	25.46211200951224	21.927936137976857
39	21.57122711811409	28.84621912096118	26.7781708068433	22.80438295408143
40	23.055251840888026	25.96343835403679	27.372788697220074	23.608521107855108
41	20.056448070963288	25.735609496194794	28.44219575590324	25.76574667693868
42	22.689902810163534	27.033668270897255	26.399964617098377	23.876464301840837
43	22.33031378668019	26.751222201536486	26.83793088197025	24.080533129813077
44	21.560324247264766	28.49640153833336	28.161909689257897	21.781364525143974
45	21.021763570217058	30.563626994273935	25.970535505816066	22.44407392969294
46	22.683114230200747	28.976127855703588	27.322697205676487	21.018060708419174
47	22.532736898297813	27.50886886829229	27.541988909928918	22.41640532348098
48	22.5353083301019	26.118135691370583	29.43672272045142	21.909833258076095
49	21.701752996489482	25.316671826673154	30.07526066604198	22.906314510795386
50	20.546665830094184	29.02591077543069	28.495372965611725	21.932050428863395
51	19.920162185346747	30.03926062078478	27.2239542243996	22.816622969468874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	530.0
1	948.0
2	1366.0
3	7798.0
4	14230.0
5	9797.5
6	5365.0
7	5310.0
8	5255.0
9	5447.0
10	5639.0
11	5540.0
12	5441.0
13	5283.0
14	5125.0
15	4945.5
16	4766.0
17	4389.0
18	4012.0
19	3940.0
20	3868.0
21	4055.0
22	4242.0
23	4922.5
24	5603.0
25	6328.5
26	8359.0
27	9664.0
28	11674.5
29	13685.0
30	15432.5
31	17180.0
32	19002.0
33	20824.0
34	23996.5
35	27169.0
36	30787.5
37	34406.0
38	41468.0
39	48530.0
40	57382.5
41	66235.0
42	71218.0
43	76201.0
44	77084.5
45	77968.0
46	78540.5
47	79113.0
48	77226.5
49	75340.0
50	72442.5
51	69545.0
52	65038.0
53	60531.0
54	55902.0
55	51273.0
56	47608.5
57	43944.0
58	41089.5
59	38235.0
60	34268.0
61	30301.0
62	25964.0
63	21627.0
64	18203.0
65	14779.0
66	11983.5
67	9188.0
68	7393.0
69	5598.0
70	4703.5
71	3809.0
72	3038.0
73	2267.0
74	1697.0
75	879.5
76	632.0
77	479.5
78	327.0
79	239.0
80	151.0
81	95.0
82	39.0
83	30.0
84	21.0
85	16.0
86	11.0
87	7.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	972221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.898711892632356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45782090151023	24.5622679510825
2	7.784908834234017	5.277967633642146
3	3.5389790637347174	3.5990049502680295
4	2.1816319497159533	2.9581805167673156
5	1.7073055237835637	2.8937729031719406
6	1.4230844822741755	2.8944438518092888
7	1.2227667181586714	2.901515168353227
8	1.0526154289256007	2.8545845727110852
9	0.9249059366969887	2.821780788828883
>10	7.632374502393932	46.162074946771604
>50	0.05809464653419609	1.2654235506928775
>100	0.01429538364303768	0.810717277522587
>500	6.083141974956236E-4	0.13073756935178668
>1k	3.041570987478118E-4	0.3134402293607504
>5k	3.041570987478118E-4	0.5540880896660108
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5374	0.5527549806062613	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3040	0.31268610737682073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010182869944179359	0.0
2	0.0	0.0	0.0	0.03270861254797006	0.0
3	0.0	0.0	0.0	0.05112006426522365	0.0
4	0.0	0.0	0.0	0.08125724500910801	0.0
5	0.0	0.0	0.0	0.15624019641624692	0.0
6	0.0	0.0	0.0	0.2534403186106863	0.0
7	0.0	0.0	0.0	0.3185489718901361	0.0
8	0.0	0.0	0.0	0.4592577202097054	0.0
9	0.0	0.0	0.0	0.6295893629123419	0.0
10	0.0	0.0	0.0	0.8917725496569195	0.0
11	0.0	0.0	0.0	1.1095213948268963	0.0
12	0.0	0.0	0.0	1.3025844946776504	0.0
13	0.0	0.0	0.0	1.3886760314784397	0.0
14	0.0	0.0	0.0	1.4278646521727056	0.0
15	0.0	0.0	0.0	1.475590426456536	0.0
16	0.0	0.0	0.0	1.5757734095437148	0.0
17	0.0	0.0	0.0	1.6841849744039679	0.0
18	0.0	0.0	0.0	1.834459449034736	0.0
19	0.0	0.0	0.0	1.9152024076830267	0.0
20	0.0	0.0	0.0	2.011373957155832	0.0
21	0.0	0.0	0.0	2.129454105599447	0.0
22	0.0	0.0	0.0	2.2461971095049376	0.0
23	0.0	0.0	0.0	2.3824829951214794	0.0
24	0.0	0.0	0.0	2.486677411823032	0.0
25	0.0	0.0	0.0	2.5875803958153547	0.0
26	0.0	0.0	0.0	2.6816947998448915	0.0
27	0.0	0.0	0.0	2.7740606302476496	0.0
28	0.0	0.0	0.0	2.88329505328521	0.0
29	0.0	0.0	0.0	2.9969523390257975	0.0
30	0.0	0.0	0.0	3.133341081914503	0.0
31	0.0	0.0	0.0	3.24967265673134	0.0
32	0.0	0.0	0.0	3.370427094251204	0.0
33	0.0	0.0	0.0	3.4880958136061655	0.0
34	0.0	0.0	0.0	3.613581685645548	0.0
35	1.0285727216342786E-4	0.0	0.0	3.761181871200067	0.0
36	1.0285727216342786E-4	0.0	0.0	3.8879020305054097	0.0
37	1.0285727216342786E-4	0.0	0.0	4.018222194336473	0.0
38	1.0285727216342786E-4	0.0	0.0	4.175285248930027	0.0
39	1.0285727216342786E-4	0.0	0.0	4.376885502370346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	45	3.8380676E-10	45.0	1
TATGCGA	105	0.0	45.0	2
GATTCGC	20	7.032751E-4	45.0	9
CAATCGA	20	7.032751E-4	45.0	2
CGTTTTT	4690	0.0	43.128998	1
CGTTAGA	80	0.0	42.1875	2
TATAGCG	65	0.0	41.53846	1
CGTAAGA	115	0.0	41.086956	2
CGGTCTA	105	0.0	40.714287	31
GACCGAT	260	0.0	40.673077	9
CTATGCG	50	1.0804797E-9	40.500004	1
GTATGCG	50	1.0804797E-9	40.500004	1
TAAGCGA	95	0.0	40.263157	2
ACGAGGT	140	0.0	40.17857	5
AGGGGAA	6940	0.0	39.488472	8
GAGCGAT	680	0.0	39.375004	7
CGATAAG	40	3.4574987E-7	39.375	10
GTTTTTA	3425	0.0	39.28467	2
GAGGGGA	6955	0.0	39.2092	7
TAGAGTA	415	0.0	39.036144	5
>>END_MODULE