FastQCFastQC Report
Thu 26 May 2016
SRR1546949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546949_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences705922
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126901.7976490320460332No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70280.9955774150685203No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9160.12975937851490674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA253.88927E-545.00000420
ACGTGCG351.2110286E-745.0000041
GTATCGG253.88927E-545.0000041
GCGGAGT351.2110286E-745.0000044
TCTGCGA406.8084773E-945.02
ATTACGC207.0314354E-445.022
CACGACG1050.045.026
ATCGTAG207.0314354E-445.01
TAGCACG207.0314354E-445.01
TCTACGA207.0314354E-445.02
ATCGGTT207.0314354E-445.039
GACGAAC207.0314354E-445.033
TAAGCGA406.8084773E-945.02
GCGTAAG207.0314354E-445.01
CGTGCGG302.1643064E-644.9999962
CGTTTTT77700.044.2471051
CGACGGT1200.043.12499628
CGAGGTT950.042.631586
GTTTTTA53900.042.4118772
TTACGAG850.042.352943