##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546949_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	705922
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797069647921443	31.0	31.0	34.0	30.0	34.0
2	32.14390683389949	33.0	31.0	34.0	30.0	34.0
3	32.051134544609745	33.0	31.0	34.0	30.0	34.0
4	35.85038007031938	37.0	35.0	37.0	35.0	37.0
5	35.877912857227855	37.0	35.0	37.0	35.0	37.0
6	35.93326599822643	37.0	35.0	37.0	35.0	37.0
7	36.15062853969702	37.0	35.0	37.0	35.0	37.0
8	13.0343437376934	0.0	0.0	36.0	0.0	37.0
9	24.82839747167534	18.0	17.0	39.0	17.0	39.0
10	32.76730998608912	32.0	32.0	38.0	27.0	39.0
11	35.64981258552645	37.0	35.0	37.0	32.0	39.0
12	36.773453724349146	38.0	35.0	39.0	33.0	39.0
13	37.005680514277785	39.0	37.0	39.0	33.0	39.0
14	38.23995285598126	40.0	37.0	41.0	34.0	41.0
15	38.50915681902533	40.0	38.0	41.0	34.0	41.0
16	38.606983491093914	40.0	38.0	41.0	34.0	41.0
17	38.58702802859239	40.0	38.0	41.0	34.0	41.0
18	38.49534651137095	40.0	38.0	41.0	34.0	41.0
19	38.43635274152102	40.0	37.0	41.0	34.0	41.0
20	38.36343817022277	40.0	37.0	41.0	34.0	41.0
21	38.28613642867059	40.0	37.0	41.0	34.0	41.0
22	38.23837336136287	40.0	37.0	41.0	34.0	41.0
23	38.063793166950454	40.0	36.0	41.0	34.0	41.0
24	37.77679970308334	40.0	35.0	41.0	34.0	41.0
25	37.66564861273625	40.0	35.0	41.0	33.0	41.0
26	37.5835163091673	39.0	35.0	41.0	33.0	41.0
27	37.52568839050207	39.0	35.0	41.0	33.0	41.0
28	37.42792688144016	39.0	35.0	41.0	33.0	41.0
29	37.37457112825496	39.0	35.0	41.0	33.0	41.0
30	37.238935746442245	39.0	35.0	41.0	33.0	41.0
31	37.07933879380442	39.0	35.0	41.0	33.0	41.0
32	36.772309121970984	39.0	35.0	41.0	31.0	41.0
33	36.44467660733056	39.0	35.0	41.0	31.0	41.0
34	36.10759120696054	39.0	35.0	41.0	29.0	41.0
35	35.854493839262695	39.0	35.0	41.0	27.0	41.0
36	35.69436424987463	39.0	35.0	41.0	25.0	41.0
37	35.614052260731356	39.0	35.0	41.0	25.0	41.0
38	35.50534761630888	39.0	35.0	41.0	24.0	41.0
39	35.44191426248226	39.0	35.0	41.0	24.0	41.0
40	35.32399330237618	39.0	35.0	41.0	23.0	41.0
41	35.24956298287913	39.0	35.0	41.0	23.0	41.0
42	35.189673929980934	39.0	35.0	41.0	23.0	41.0
43	35.07657361578191	38.0	35.0	40.0	23.0	41.0
44	34.86407421783143	38.0	35.0	40.0	22.0	41.0
45	34.78125911927947	38.0	34.0	40.0	23.0	41.0
46	34.74262028949374	38.0	34.0	40.0	22.0	41.0
47	34.70303942928539	38.0	34.0	40.0	22.0	41.0
48	34.61860233850199	38.0	34.0	40.0	22.0	41.0
49	34.52835157425324	38.0	34.0	40.0	21.0	41.0
50	34.421199509294226	38.0	34.0	40.0	20.0	41.0
51	34.08073129892538	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	8.0
16	31.0
17	77.0
18	218.0
19	498.0
20	880.0
21	1628.0
22	2674.0
23	4213.0
24	7083.0
25	10911.0
26	14081.0
27	14494.0
28	13584.0
29	13010.0
30	14152.0
31	17352.0
32	21953.0
33	30581.0
34	47068.0
35	77997.0
36	72334.0
37	118777.0
38	158219.0
39	64076.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.252036343958682	22.21194409580662	25.696040072415933	20.83997948781877
2	38.27009216315684	23.443666580727047	26.19127892316715	12.094962332948967
3	28.122653777612822	23.046880533543366	36.53123149583099	12.299234193012826
4	24.69989602250674	25.279280147098405	35.41694408164075	14.603879748754112
5	21.81614965959412	29.46062029516009	35.101611792804306	13.62161825244149
6	20.082672023254695	37.41702341051844	32.820198265530756	9.68010630069611
7	79.62182790733254	4.344247664756163	14.215734882890743	1.8181895450205547
8	29.566864327786924	64.38501704154284	5.128895260382875	0.9192233702873689
9	76.24085947172634	5.526814577247912	15.13708313383065	3.0952428171951007
10	42.036230631712854	26.860333011295868	20.863636492417008	10.239799864574273
11	35.76910763512116	22.51495207685835	28.049274565745225	13.666665722275265
12	32.534189329699316	21.83853173580084	31.3941200302583	14.233158904241543
13	21.186618351602586	31.85040273571301	31.22922929162145	15.73374962106295
14	15.79749604063905	34.59716512589209	34.04342122784104	15.561917605627817
15	15.362887117840215	24.97060581763991	44.62773507554659	15.038771988973288
16	17.288737282589295	22.051586435895185	44.49372593572661	16.16595034578891
17	17.589195406857982	21.86728845396517	34.2017956658101	26.34172047336675
18	20.99707899739631	25.62336915409918	35.551944832431914	17.827607016072598
19	24.618867240290005	26.85693320225181	30.476171588362455	18.048027969095738
20	26.507886140395115	24.80288190479968	30.59020685004859	18.099025104756617
21	21.797875685982305	26.831717951841703	32.90051875419664	18.469887607979352
22	20.86391980983735	24.69465465023048	32.76806786018852	21.673357679743653
23	19.1195344528149	28.769750765665332	32.10722997724961	20.003484804270162
24	19.188380585957088	25.37886622034729	38.19331314224518	17.239440051450444
25	18.68875031519063	26.203886548372203	35.83384566566845	19.273517470768724
26	16.972413382781667	31.262802405931534	31.93681454891617	19.82796966237063
27	18.02635418643986	29.558789781307283	34.792087511084794	17.622768521168062
28	15.833902329152513	28.251846521287057	39.37403849150473	16.540212658055705
29	16.437651751893267	27.17878179175603	38.331005408529556	18.052561047821147
30	18.540858621774078	27.452608078512924	36.550922056544486	17.45561124316851
31	25.349117891211776	26.32514640427696	30.86927450908174	17.456461195429522
32	24.41686191958885	27.873334447715187	31.583659384464575	16.12614424823139
33	22.772912588076302	27.977311940979316	30.669252410322954	18.580523060621427
34	18.31689620099671	29.06298429571539	32.922475854272854	19.69764364901505
35	18.909454585634105	27.741166871127405	33.11201520847912	20.237363334759365
36	23.92998093273761	28.636733236816532	29.851315017806503	17.58197081263936
37	19.974869744815997	30.767563555180317	30.942370403529	18.31519629647468
38	19.067687364893004	32.727270151659816	28.184558633956726	20.020483849490454
39	20.119361629188496	29.94056000521304	29.391774162017896	20.548304203580567
40	21.1874683038636	28.057632429645203	29.635002167378268	21.119897099112933
41	17.308002867172295	27.57485954538887	31.219454840619786	23.897682746819054
42	20.578760826266926	29.487535450092217	27.586192242202394	22.34751148143846
43	21.269488697051518	29.386391131031473	27.963146069962402	21.380974101954607
44	19.487705440544424	31.78198157870133	29.64930969710535	19.0810032836489
45	19.074345324270954	33.546340813857626	26.73241519601316	20.646898665858266
46	20.84904564526959	31.80620521814025	28.529497593218515	18.815251543371648
47	21.322185737234424	29.817033609945575	29.26739781448942	19.59338283833058
48	20.861369953054304	28.173650913273708	31.29042585441451	19.67455327925748
49	20.50453166213831	27.781397944815435	30.97041882814249	20.74365156490377
50	18.006946943146694	31.19013148761478	30.481554619348877	20.321366949889647
51	18.460821450528528	31.709735636515084	28.711529035785823	21.117913877170565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	718.0
1	1140.5
2	1563.0
3	14593.5
4	27624.0
5	17743.5
6	7863.0
7	7797.0
8	7731.0
9	8207.5
10	8684.0
11	8948.5
12	9213.0
13	8960.0
14	8707.0
15	8211.5
16	7716.0
17	7207.0
18	6698.0
19	6310.0
20	5922.0
21	5761.0
22	5600.0
23	5926.5
24	6253.0
25	6775.5
26	8483.0
27	9668.0
28	10910.0
29	12152.0
30	13368.0
31	14584.0
32	16061.5
33	17539.0
34	18893.0
35	20247.0
36	22088.0
37	23929.0
38	29375.5
39	34822.0
40	40858.0
41	46894.0
42	49764.0
43	52634.0
44	52517.0
45	52400.0
46	51789.0
47	51178.0
48	49608.0
49	48038.0
50	45455.0
51	42872.0
52	39668.0
53	36464.0
54	33989.0
55	31514.0
56	29223.5
57	26933.0
58	24898.0
59	22863.0
60	20048.5
61	17234.0
62	14837.5
63	12441.0
64	9924.5
65	7408.0
66	5992.0
67	4576.0
68	3785.5
69	2995.0
70	2567.5
71	2140.0
72	1758.5
73	1377.0
74	1074.5
75	557.5
76	343.0
77	260.0
78	177.0
79	128.0
80	79.0
81	51.5
82	24.0
83	19.0
84	14.0
85	11.0
86	8.0
87	7.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.170798843704404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81172950172365	23.82052433920188
2	7.618427303772026	5.054186391976143
3	3.7330241970141405	3.714821841535116
4	2.46500210775154	3.2706435626213475
5	1.851684308541306	3.071092386033377
6	1.5330734282769454	3.051196218120171
7	1.2964964318935541	3.0104075640745114
8	1.1119896141465577	2.9508467045715157
9	1.007807031086154	3.008678787128679
>10	7.478711231365849	43.148861890475715
>50	0.0653847493757131	1.4345896283702286
>100	0.024089118112025777	1.2730663050413078
>500	0.0017206512937161268	0.377553625099675
>1k	0.0	0.0
>5k	4.301628234290317E-4	1.0028143904763855
>10k+	4.301628234290317E-4	1.8107163652739515
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12690	1.7976490320460332	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7028	0.9955774150685203	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	916	0.12975937851490674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005383030986426262	0.0
2	0.0	0.0	0.0	0.02025719555418304	0.0
3	0.0	0.0	0.0	0.036831264643969165	0.0
4	0.0	0.0	0.0	0.053405333733755285	0.0
5	0.0	0.0	0.0	0.10199427132176075	0.0
6	0.0	0.0	0.0	0.18118149030629446	0.0
7	0.0	0.0	0.0	0.24393629891121116	0.0
8	0.0	0.0	0.0	0.3490470618566924	0.0
9	0.0	0.0	0.0	0.5764092916781174	0.0
10	0.0	0.0	0.0	0.8350780964469162	0.0
11	0.0	0.0	0.0	1.0931802663750385	0.0
12	0.0	0.0	0.0	1.2902275322202736	0.0
13	0.0	0.0	0.0	1.3834389635115494	0.0
14	0.0	0.0	0.0	1.4266448701131287	0.0
15	0.0	0.0	0.0	1.470134094135046	0.0
16	0.0	0.0	0.0	1.5501712653805944	0.0
17	0.0	0.0	0.0	1.6398412289176425	0.0
18	0.0	0.0	0.0	1.7659174809681524	0.0
19	0.0	0.0	0.0	1.83008887667476	0.0
20	0.0	0.0	0.0	1.8948269072220443	0.0
21	1.4165871016911217E-4	0.0	0.0	1.9728808565252252	0.0
22	1.4165871016911217E-4	0.0	0.0	2.0580177413368617	0.0
23	1.4165871016911217E-4	0.0	0.0	2.147971022294248	0.0
24	1.4165871016911217E-4	0.0	0.0	2.2171004728567745	0.0
25	1.4165871016911217E-4	0.0	0.0	2.2750388853159413	0.0
26	1.4165871016911217E-4	0.0	0.0	2.331560710673417	0.0
27	1.4165871016911217E-4	0.0	0.0	2.3999818676850984	0.0
28	1.4165871016911217E-4	0.0	0.0	2.470102929218809	0.0
29	1.4165871016911217E-4	0.0	0.0	2.5386657449406593	0.0
30	1.4165871016911217E-4	0.0	0.0	2.636976889798023	0.0
31	1.4165871016911217E-4	0.0	0.0	2.714180886840189	0.0
32	1.4165871016911217E-4	0.0	0.0	2.7983261606806416	0.0
33	1.4165871016911217E-4	0.0	0.0	2.8748218641719623	0.0
34	1.4165871016911217E-4	0.0	0.0	2.9558506463886944	0.0
35	1.4165871016911217E-4	0.0	0.0	3.052461886724029	0.0
36	1.4165871016911217E-4	0.0	0.0	3.1387320412170183	0.0
37	1.4165871016911217E-4	0.0	0.0	3.226418782811699	0.0
38	1.4165871016911217E-4	0.0	0.0	3.3282713954232905	0.0
39	1.4165871016911217E-4	0.0	0.0	3.4366403087026614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTATA	25	3.88927E-5	45.000004	20
ACGTGCG	35	1.2110286E-7	45.000004	1
GTATCGG	25	3.88927E-5	45.000004	1
GCGGAGT	35	1.2110286E-7	45.000004	4
TCTGCGA	40	6.8084773E-9	45.0	2
ATTACGC	20	7.0314354E-4	45.0	22
CACGACG	105	0.0	45.0	26
ATCGTAG	20	7.0314354E-4	45.0	1
TAGCACG	20	7.0314354E-4	45.0	1
TCTACGA	20	7.0314354E-4	45.0	2
ATCGGTT	20	7.0314354E-4	45.0	39
GACGAAC	20	7.0314354E-4	45.0	33
TAAGCGA	40	6.8084773E-9	45.0	2
GCGTAAG	20	7.0314354E-4	45.0	1
CGTGCGG	30	2.1643064E-6	44.999996	2
CGTTTTT	7770	0.0	44.247105	1
CGACGGT	120	0.0	43.124996	28
CGAGGTT	95	0.0	42.63158	6
GTTTTTA	5390	0.0	42.411877	2
TTACGAG	85	0.0	42.35294	3
>>END_MODULE