FastQCFastQC Report
Thu 26 May 2016
SRR1546948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546948_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1371988
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.2432965885999003No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21850.1592579526934638No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.14096333204080502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA207.0337637E-445.00000412
CTATGCG406.8175723E-945.0000041
TAACGCG453.8562575E-1045.0000041
TCGATAG1050.045.0000041
CATACCG207.0337637E-445.0000041
CTACCGT207.0337637E-445.00000426
TCGTACC207.0337637E-445.00000430
TAATGCG207.0337637E-445.0000041
TACGTAT207.0337637E-445.00000412
TCGTTAG850.045.01
GTCGAAA253.8912032E-545.021
AGGCCGT351.2121563E-745.024
ACGTAAG253.8912032E-545.01
TATCGCG351.2121563E-745.01
CGAGGTA302.1658107E-644.9999966
CGTCGGT302.1658107E-644.99999645
ACCCGTA302.1658107E-644.99999620
TTCGCAT302.1658107E-644.99999629
CGTTTTT34900.043.0659031
GAGGGGA150150.041.6133887
Produced by FastQC (version 0.11.5)