##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546948_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1371988
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.762852882095178	31.0	31.0	34.0	30.0	34.0
2	32.11218392580693	33.0	31.0	34.0	30.0	34.0
3	32.14343857234903	33.0	31.0	34.0	30.0	34.0
4	35.88099895917457	37.0	35.0	37.0	35.0	37.0
5	35.854023504578755	37.0	35.0	37.0	35.0	37.0
6	35.90471345230425	37.0	35.0	37.0	35.0	37.0
7	36.10886757027029	37.0	35.0	37.0	35.0	37.0
8	13.240531987160237	0.0	0.0	35.0	0.0	37.0
9	24.919653087344788	18.0	17.0	39.0	17.0	39.0
10	32.724203856010405	32.0	32.0	38.0	27.0	39.0
11	35.62322119435447	37.0	35.0	37.0	32.0	39.0
12	36.57014274177325	37.0	35.0	39.0	33.0	39.0
13	36.69629253317085	39.0	35.0	39.0	33.0	39.0
14	37.883881637448724	40.0	36.0	41.0	33.0	41.0
15	38.214728554477155	40.0	37.0	41.0	33.0	41.0
16	38.332625358239284	40.0	37.0	41.0	34.0	41.0
17	38.32299262092671	40.0	37.0	41.0	34.0	41.0
18	38.28307244669779	40.0	37.0	41.0	34.0	41.0
19	38.25397962664397	40.0	37.0	41.0	34.0	41.0
20	38.229721396980146	40.0	37.0	41.0	34.0	41.0
21	38.15651157298752	40.0	36.0	41.0	34.0	41.0
22	38.08957877182599	40.0	36.0	41.0	33.0	41.0
23	38.01744403012271	40.0	36.0	41.0	34.0	41.0
24	37.95068032665009	40.0	36.0	41.0	34.0	41.0
25	37.85248486138363	40.0	35.0	41.0	33.0	41.0
26	37.766086146526064	40.0	35.0	41.0	33.0	41.0
27	37.704432546057255	40.0	35.0	41.0	33.0	41.0
28	37.66049775945562	40.0	35.0	41.0	33.0	41.0
29	37.70661259427925	40.0	36.0	41.0	33.0	41.0
30	37.62906891313918	40.0	36.0	41.0	33.0	41.0
31	37.5704007615227	40.0	35.0	41.0	33.0	41.0
32	37.41753499301743	40.0	35.0	41.0	33.0	41.0
33	37.28864027965259	40.0	35.0	41.0	33.0	41.0
34	37.17501975235935	40.0	35.0	41.0	32.0	41.0
35	37.084674209978516	40.0	35.0	41.0	32.0	41.0
36	36.97709892506349	40.0	35.0	41.0	32.0	41.0
37	36.90624772228328	40.0	35.0	41.0	31.0	41.0
38	36.83832147219947	40.0	35.0	41.0	31.0	41.0
39	36.79478610600093	40.0	35.0	41.0	31.0	41.0
40	36.65806406470028	39.0	35.0	41.0	31.0	41.0
41	36.634230765866754	39.0	35.0	41.0	31.0	41.0
42	36.6036065913113	39.0	35.0	41.0	31.0	41.0
43	36.5009919911836	39.0	35.0	41.0	31.0	41.0
44	36.26226905774686	39.0	35.0	41.0	30.0	41.0
45	36.18473193643093	39.0	35.0	40.0	30.0	41.0
46	36.16740306766531	39.0	35.0	40.0	30.0	41.0
47	36.13681096336119	39.0	35.0	40.0	30.0	41.0
48	36.09710726332884	38.0	35.0	40.0	30.0	41.0
49	36.00367058604011	38.0	35.0	40.0	30.0	41.0
50	35.89293055041298	38.0	35.0	40.0	29.0	41.0
51	35.50563270232684	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	7.0
14	9.0
15	32.0
16	49.0
17	127.0
18	292.0
19	645.0
20	1172.0
21	2088.0
22	3241.0
23	5042.0
24	7504.0
25	10961.0
26	14104.0
27	16178.0
28	17314.0
29	20109.0
30	25297.0
31	33404.0
32	44390.0
33	63096.0
34	99194.0
35	156393.0
36	142104.0
37	242661.0
38	338292.0
39	128225.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.7182336871751	24.20750035714598	27.810665982501305	13.263599973177609
2	32.40494814823453	26.183465161502873	29.09879678247915	12.31278990778345
3	30.12424306918136	25.52201622754718	30.87782108881419	13.475919614457268
4	28.290917996367316	27.412630431169955	29.305212582034244	14.991238990428487
5	23.923897293562334	32.401158027621236	28.193759712184068	15.48118496663236
6	22.378694274293945	41.3655950343589	25.592570780502456	10.663139910844702
7	86.6416470114899	5.030218923197579	6.522797575488999	1.8053364898235262
8	32.704877885229315	63.95850401023916	2.3006032122729936	1.0360148922585328
9	82.89934022746554	6.159018883547087	7.587675693956506	3.3539651950308604
10	51.24017119683263	25.078207681116744	12.755213602451335	10.926407519599298
11	45.4023650352627	21.26862625620632	19.163651577127496	14.16535713140348
12	41.0631871415785	21.285244477356947	23.51755263165567	14.134015749408887
13	22.640285483546503	37.17743886972772	22.84655550923186	17.335720137493915
14	16.168946084076538	39.870028017737766	26.969550754088228	16.99147514409747
15	16.09686090548897	24.99125356781546	43.32705533867643	15.584830188019136
16	17.595634947244438	20.210818170421316	42.79410607089858	19.39944081143567
17	18.13616445624889	20.758053277433913	27.625533167928584	33.48024909838861
18	22.17876541194238	24.967856861721824	31.399545768621884	21.453831957713916
19	28.6272183138628	26.60890620034578	24.431846342679382	20.332029143112038
20	31.778703603821608	23.48985559640463	24.487094639311714	20.244346160462044
21	24.13067752779179	28.477800097376942	25.784700740822807	21.606821634008462
22	23.282856701370566	24.88848298964714	25.65335848418499	26.1753018247973
23	22.662443111747333	28.72386638950195	24.20298136718397	24.410709131566747
24	22.10675312028968	24.498610775021355	34.610288136630935	18.78434796805803
25	19.60286824666105	25.452627865549843	32.547587879777375	22.396916008011733
26	17.883392566115738	33.517931643717006	26.37588666956271	22.222789120604553
27	19.098199109613205	32.437309947317324	28.19929911923428	20.265191823835195
28	16.5947515575938	28.870004693918606	36.034498844013214	18.500744904474384
29	17.487179188156166	25.833024778642375	35.18973926885658	21.490056764344878
30	19.776120490849774	29.830217173911144	30.49531045461039	19.89835188062869
31	29.799896209004746	26.39498304649895	23.43759566410202	20.367525080394287
32	29.76607667122453	27.752866643148483	24.208375000364434	18.272681685262555
33	27.848640075569175	28.004107907649338	23.72265646638309	20.4245955503984
34	20.425470193616853	28.747846191074554	27.194188287361115	23.632495327947474
35	21.52569847549687	26.042210281722582	29.817024638699465	22.615066604081086
36	28.272404714910042	27.05898302317513	25.715020831085987	18.953591430828844
37	22.15398385408619	31.422286492301684	27.324145692236375	19.099583961375753
38	20.683708603865337	32.01383685571594	24.23782132205238	23.064633218366343
39	21.91148902176987	29.99494164671994	26.119761980425483	21.973807351084705
40	23.657495546608278	26.607448461648353	26.415537162132612	23.319518829610754
41	18.06961868471153	25.7369597984822	27.648638326282736	28.544783190523532
42	21.873296267897388	26.7566480173296	23.905456899039933	27.464598815733083
43	23.31871707332717	26.13535978448791	24.811077064813976	25.73484607737094
44	20.38407041461004	29.75084330183646	27.205850196940496	22.659236086613003
45	19.636104688962295	34.78215552905711	22.95049227835812	22.631247503622482
46	22.866672303256298	32.65626229967026	24.724341612317307	19.752723784756135
47	22.450123470467673	27.705344361612493	26.669985451767797	23.174546716152037
48	23.282200718956723	24.348901010796013	29.994504325110714	22.374393945136546
49	21.0872835622469	25.60051545640341	29.771980512949092	23.540220468400598
50	19.445869788948592	30.412583783531634	28.357026446295446	21.784519981224328
51	19.152135441417855	32.05990139855451	25.68302346667755	23.104939693350087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	753.0
1	1507.0
2	2261.0
3	6247.5
4	10234.0
5	7573.5
6	4913.0
7	5073.0
8	5233.0
9	5298.5
10	5364.0
11	5354.5
12	5345.0
13	5012.5
14	4680.0
15	4497.5
16	4315.0
17	4234.0
18	4153.0
19	4180.5
20	4208.0
21	4780.5
22	5353.0
23	5594.0
24	5835.0
25	7586.5
26	12869.5
27	16401.0
28	18856.0
29	21311.0
30	24234.0
31	27157.0
32	30052.5
33	32948.0
34	34105.5
35	35263.0
36	38731.5
37	42200.0
38	56636.0
39	71072.0
40	88154.5
41	105237.0
42	114045.0
43	122853.0
44	120014.5
45	117176.0
46	113457.0
47	109738.0
48	105310.0
49	100882.0
50	96389.5
51	91897.0
52	85257.5
53	78618.0
54	74420.5
55	70223.0
56	68460.5
57	66698.0
58	62471.5
59	58245.0
60	52420.5
61	46596.0
62	39112.5
63	31629.0
64	25674.5
65	19720.0
66	16157.0
67	12594.0
68	10334.5
69	8075.0
70	6774.5
71	5474.0
72	4625.5
73	3777.0
74	2962.0
75	1677.0
76	1207.0
77	906.5
78	606.0
79	384.5
80	163.0
81	101.0
82	39.0
83	31.5
84	24.0
85	20.5
86	17.0
87	11.5
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1371988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.910397217775927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0994425118852	16.121799681925992
2	5.553845505727329	2.3226623122183616
3	1.969256526148231	1.2353380855637117
4	1.2182767905756113	1.01898606448533
5	0.8898784032901431	0.9303855444158546
6	0.7527953295721662	0.9444749619024322
7	0.6525002328494787	0.9550827337501719
8	0.5609441871955709	0.9383652617009476
9	0.5143916454606009	0.9680520268877878
>10	9.013061190400794	46.5426415905109
>50	1.6254721825669647	22.50254156853688
>100	0.14626783522344475	4.533444984819748
>500	0.0024612376119316	0.3634931643168315
>1k	0.0014064214925323427	0.6227320189651219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3338	0.2432965885999003	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2185	0.1592579526934638	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1934	0.14096333204080502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016108012606524256	0.0
2	0.0	0.0	0.0	0.04344061318320568	0.0
3	0.0	0.0	0.0	0.06319297253328747	0.0
4	0.0	0.0	0.0	0.10204170881961067	0.0
5	0.0	0.0	0.0	0.1951183246500698	0.0
6	0.0	0.0	0.0	0.2738362143109123	0.0
7	0.0	0.0	0.0	0.3271165637017233	0.0
8	0.0	0.0	0.0	0.4259512473870034	0.0
9	0.0	0.0	0.0	0.525077478811768	0.0
10	0.0	0.0	0.0	0.7591903136179033	0.0
11	0.0	0.0	0.0	0.980839482561072	0.0
12	0.0	0.0	0.0	1.1899520987064027	0.0
13	0.0	0.0	0.0	1.277780855226139	0.0
14	0.0	0.0	0.0	1.309268011090476	0.0
15	0.0	0.0	0.0	1.3632043428951273	0.0
16	0.0	0.0	0.0	1.479750551754097	0.0
17	0.0	0.0	0.0	1.604241436514022	0.0
18	0.0	0.0	0.0	1.789228477217002	0.0
19	0.0	0.0	0.0	1.8824508669172033	0.0
20	0.0	0.0	0.0	1.9734137616363991	0.0
21	0.0	0.0	0.0	2.099653932833232	0.0
22	0.0	0.0	0.0	2.2264043125741626	0.0
23	0.0	0.0	0.0	2.369627139595973	0.0
24	0.0	0.0	0.0	2.4715959614807126	0.0
25	0.0	0.0	0.0	2.56139266524197	0.0
26	0.0	0.0	0.0	2.649877404175547	0.0
27	0.0	0.0	0.0	2.732749849124045	0.0
28	0.0	0.0	0.0	2.8268468820427	0.0
29	0.0	0.0	0.0	2.9221101059192938	0.0
30	0.0	0.0	0.0	3.039458071061846	0.0
31	0.0	0.0	0.0	3.160377496013085	0.0
32	0.0	0.0	0.0	3.2756117400443734	0.0
33	0.0	0.0	0.0	3.3820266649562534	0.0
34	0.0	0.0	0.0	3.5070277582602762	0.0
35	0.0	0.0	0.0	3.658122374248171	0.0
36	0.0	0.0	0.0	3.783852336900906	0.0
37	0.0	0.0	0.0	3.8995239025414214	0.0
38	0.0	0.0	0.0	4.018985588795237	0.0
39	0.0	0.0	0.0	4.149817636888953	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.0337637E-4	45.000004	12
CTATGCG	40	6.8175723E-9	45.000004	1
TAACGCG	45	3.8562575E-10	45.000004	1
TCGATAG	105	0.0	45.000004	1
CATACCG	20	7.0337637E-4	45.000004	1
CTACCGT	20	7.0337637E-4	45.000004	26
TCGTACC	20	7.0337637E-4	45.000004	30
TAATGCG	20	7.0337637E-4	45.000004	1
TACGTAT	20	7.0337637E-4	45.000004	12
TCGTTAG	85	0.0	45.0	1
GTCGAAA	25	3.8912032E-5	45.0	21
AGGCCGT	35	1.2121563E-7	45.0	24
ACGTAAG	25	3.8912032E-5	45.0	1
TATCGCG	35	1.2121563E-7	45.0	1
CGAGGTA	30	2.1658107E-6	44.999996	6
CGTCGGT	30	2.1658107E-6	44.999996	45
ACCCGTA	30	2.1658107E-6	44.999996	20
TTCGCAT	30	2.1658107E-6	44.999996	29
CGTTTTT	3490	0.0	43.065903	1
GAGGGGA	15015	0.0	41.613388	7
>>END_MODULE