FastQCFastQC Report
Thu 26 May 2016
SRR1546947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546947_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1098758
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143401.3051099514178737No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80280.7306431443502572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2118653E-745.0000041
ATTCGCG207.033149E-445.0000041
CGTTTTT105000.044.185721
AGCGATA1250.043.28
ACGGTCT1750.042.42857430
GTTTTTA72850.042.127662
TCACGAC1800.040.025
TTAGCGA1300.039.807692
GTTACGA800.039.3750042
TACGCGG403.4580262E-739.3750042
GTATGCG403.4580262E-739.3750041
CGAGGTA750.039.06
GAGCGAT7150.038.7062957
GCGATAA700.038.571439
CACGACG1900.037.89473726
CTCGTCA301.13988935E-437.49999621
TTATTTT84850.037.4425436
ATACGAG1150.037.1739163
CGGTCTA2000.037.12531
TTTTATT86150.037.0603564
Produced by FastQC (version 0.11.5)