##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546947_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1098758
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7894568230675	31.0	31.0	34.0	30.0	34.0
2	32.142165972852986	33.0	31.0	34.0	30.0	34.0
3	32.07377966758831	33.0	31.0	34.0	30.0	34.0
4	35.87432719488732	37.0	35.0	37.0	35.0	37.0
5	35.89642123197283	37.0	35.0	37.0	35.0	37.0
6	35.94736784624094	37.0	35.0	37.0	35.0	37.0
7	36.160527613905884	37.0	35.0	37.0	35.0	37.0
8	13.306595264835387	0.0	0.0	37.0	0.0	37.0
9	24.97872324934153	18.0	17.0	39.0	17.0	39.0
10	32.77536909856401	32.0	32.0	38.0	27.0	39.0
11	35.65210901763628	37.0	35.0	37.0	32.0	39.0
12	36.78081069716899	38.0	35.0	39.0	33.0	39.0
13	37.08051090412994	39.0	37.0	39.0	33.0	39.0
14	38.28885341449163	40.0	38.0	41.0	34.0	41.0
15	38.53747595011822	40.0	38.0	41.0	34.0	41.0
16	38.589800483819005	40.0	38.0	41.0	34.0	41.0
17	38.54909816356286	40.0	38.0	41.0	34.0	41.0
18	38.4305989125904	40.0	38.0	41.0	34.0	41.0
19	38.390127762437224	40.0	37.0	41.0	34.0	41.0
20	38.336957728635426	40.0	37.0	41.0	34.0	41.0
21	38.29174304077877	40.0	37.0	41.0	34.0	41.0
22	38.250276220969496	40.0	37.0	41.0	34.0	41.0
23	38.08432521082896	40.0	37.0	41.0	34.0	41.0
24	37.83797888160996	40.0	35.0	41.0	34.0	41.0
25	37.732954845379965	40.0	35.0	41.0	33.0	41.0
26	37.6689935363383	40.0	35.0	41.0	33.0	41.0
27	37.61806603455902	40.0	35.0	41.0	33.0	41.0
28	37.501009321433834	40.0	35.0	41.0	33.0	41.0
29	37.45307064885989	40.0	35.0	41.0	33.0	41.0
30	37.290072973302586	40.0	35.0	41.0	33.0	41.0
31	37.14824101394483	40.0	35.0	41.0	33.0	41.0
32	36.89350339201171	40.0	35.0	41.0	31.0	41.0
33	36.57536418392403	40.0	35.0	41.0	31.0	41.0
34	36.220919438129236	40.0	35.0	41.0	29.0	41.0
35	35.94425979151005	40.0	35.0	41.0	27.0	41.0
36	35.8066307594575	39.0	35.0	41.0	26.0	41.0
37	35.75182797303865	39.0	35.0	41.0	25.0	41.0
38	35.64530588173192	39.0	35.0	41.0	25.0	41.0
39	35.54002337184348	39.0	35.0	41.0	24.0	41.0
40	35.44826613321587	39.0	35.0	41.0	24.0	41.0
41	35.36650108577139	39.0	35.0	41.0	23.0	41.0
42	35.29589682168412	39.0	35.0	41.0	23.0	41.0
43	35.19831391443794	39.0	35.0	40.0	23.0	41.0
44	35.00149441460267	38.0	35.0	40.0	23.0	41.0
45	34.94452645623513	38.0	34.0	40.0	23.0	41.0
46	34.91274602778774	38.0	34.0	40.0	23.0	41.0
47	34.87949484781908	38.0	34.0	40.0	23.0	41.0
48	34.80430995724263	38.0	34.0	40.0	23.0	41.0
49	34.70109068602913	38.0	34.0	40.0	23.0	41.0
50	34.60354600376061	38.0	34.0	40.0	22.0	41.0
51	34.26934684434607	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	8.0
14	9.0
15	31.0
16	47.0
17	156.0
18	333.0
19	655.0
20	1296.0
21	2248.0
22	3781.0
23	6273.0
24	10259.0
25	15998.0
26	21224.0
27	22872.0
28	21408.0
29	20830.0
30	22397.0
31	26717.0
32	34530.0
33	47281.0
34	71615.0
35	106627.0
36	115146.0
37	194020.0
38	243378.0
39	109594.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36954816256173	21.59356291376263	26.132232939373367	19.904655984302273
2	38.10475100067531	22.634738495646904	27.08066744451462	12.179843059163165
3	28.411078690667097	23.234961656706936	35.88306069216333	12.470898960462632
4	25.35735803516334	25.198087294927547	35.18936835954778	14.255186310361335
5	22.459631693239093	29.214076257010184	34.178135676827836	14.148156372922882
6	20.351433163626567	38.48090298318647	31.40109105007654	9.76657280311042
7	80.58962938153806	3.9155118779567477	13.483041761698209	2.0118169788069804
8	30.396957291778538	63.58078849027721	5.017028317427495	1.0052259005167654
9	76.42802145695413	5.145719075538016	15.016773484243117	3.409485983264741
10	40.33253910324203	26.663924176206226	21.53495128135586	11.468585439195893
11	33.33054230321872	22.711188450960083	28.726070708927715	15.232198536893474
12	29.93661934657131	21.055955906578156	33.11393409649804	15.893490650352488
13	23.695117578211033	24.55135707771866	33.76830930923825	17.985216034832057
14	18.373927652858953	27.563303293354863	35.94221839567949	18.120550658106698
15	18.54785130119644	23.59700680222579	41.25275993439866	16.602381962179116
16	23.29448340762934	21.76020561397505	38.24409014541874	16.701220832976873
17	22.6538509844752	21.386966010713916	33.48071185829819	22.4784711465127
18	23.46158116709958	23.21229970566767	35.19619424841503	18.129924878817718
19	25.68208832154123	25.015153473285288	31.409646164123494	17.893112041049985
20	26.857779420036078	24.593768600547165	30.954313870752248	17.594138108664513
21	25.447368756359452	24.007379240924752	33.63798033779959	16.907271664916205
22	23.129023861487244	21.66746453723204	34.74159004985629	20.461921551424425
23	22.21153338587751	24.12214518574609	33.793155544715034	19.873165883661372
24	22.798286792906172	23.091709002346285	35.31942429543175	18.79057990931579
25	22.293899111542306	23.60073828813988	33.81272309280114	20.292639507516668
26	19.996304918826528	26.408180873313324	32.409593377249585	21.185920830610563
27	19.333556615742502	25.252512382162408	35.64788606772374	19.766044934371354
28	18.983616046481572	25.282728316881425	36.290247716057586	19.443407920579418
29	20.767903396380277	25.430895611226497	35.15951647223501	18.641684520158215
30	22.488118402778408	24.01111072683885	35.21931125871211	18.28145961167063
31	25.42361466310143	23.533571541686158	32.25623840736541	18.786575387847005
32	25.89869652826191	24.770240580728423	31.822657946517797	17.508404944491872
33	25.631030672814216	24.304259900724272	30.79495211866489	19.26975730779662
34	21.953423774843962	25.923269728184007	32.655780435728346	19.467526061243696
35	21.25272352965803	27.105422668139845	31.991120883761482	19.65073291844064
36	24.57502015912512	27.610629456167786	29.177580504533303	18.636769880173794
37	22.537173790771035	28.99992537028172	29.18659067783807	19.27631016110918
38	22.93234725025893	29.57457420105246	27.351609726618598	20.14146882207001
39	22.286436139714112	27.850991756146488	28.340453493853968	21.522118610285432
40	22.94672712280593	25.413603359429466	29.690432288092556	21.949237229672047
41	20.272980947578993	26.173552319983106	29.88820104153963	23.66526569089827
42	21.539228838379334	27.328583728173083	28.466777943823846	22.665409489623737
43	21.325897058314933	26.16618036000648	28.79869816647524	23.709224415203348
44	20.693364689949924	27.181690599749896	30.299665622457354	21.82527908784282
45	21.55069633167631	28.11947671825825	28.63624201143473	21.693584938630707
46	22.580859479521422	27.496318570604267	29.768793492288566	20.154028457585746
47	21.371311972245024	27.257685495805262	30.182806405050066	21.188196126899644
48	21.341551096783824	26.05678411442738	31.400818014521853	21.200846774266946
49	21.984094768820796	24.943345122401837	32.08713838716078	20.98542172161659
50	20.56485595554253	26.491183681939063	31.53988412371059	21.404076238807818
51	20.02024103578768	27.60525975692555	30.83035572892302	21.544143478363754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	810.0
1	1684.0
2	2558.0
3	20121.5
4	37685.0
5	25036.5
6	12388.0
7	11950.0
8	11512.0
9	11424.0
10	11336.0
11	10960.5
12	10585.0
13	10079.5
14	9574.0
15	8806.0
16	8038.0
17	7542.0
18	7046.0
19	6742.5
20	6439.0
21	6774.5
22	7110.0
23	7562.0
24	8014.0
25	8954.5
26	10978.0
27	12061.0
28	14202.0
29	16343.0
30	18859.0
31	21375.0
32	24432.5
33	27490.0
34	29610.5
35	31731.0
36	34534.0
37	37337.0
38	42481.0
39	47625.0
40	53227.0
41	58829.0
42	62681.0
43	66533.0
44	68269.5
45	70006.0
46	71848.5
47	73691.0
48	72918.0
49	72145.0
50	69958.5
51	67772.0
52	64168.5
53	60565.0
54	57656.0
55	54747.0
56	54384.5
57	54022.0
58	52702.0
59	51382.0
60	46682.0
61	41982.0
62	37331.0
63	32680.0
64	27485.5
65	22291.0
66	18143.0
67	13995.0
68	11286.0
69	8577.0
70	7132.0
71	5687.0
72	4571.0
73	3455.0
74	2615.5
75	1335.5
76	895.0
77	650.0
78	405.0
79	304.0
80	203.0
81	140.5
82	78.0
83	60.5
84	43.0
85	33.5
86	24.0
87	20.0
88	16.0
89	10.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1098758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.36535428783262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.94487414148168	22.336394434849005
2	7.862841626258262	5.246929931384497
3	4.7668187741170245	4.771397916729195
4	3.427129471677928	4.573895560512268
5	2.7533791303239328	4.593373508599124
6	2.220120803547074	4.444507030328145
7	1.8504828980542156	4.32194122480079
8	1.5538799274567394	4.147660344027661
9	1.3569171829667108	4.074662029403889
>10	7.202352297391953	36.980867047451724
>50	0.03595555857791402	0.8307728149067025
>100	0.024150440847242615	1.5255032122872496
>500	5.488736496839922E-4	0.10392833945696008
>1k	0.0	0.0
>5k	2.744368248419961E-4	0.7350984221678198
>10k+	2.744368248419961E-4	1.3130681830949846
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14340	1.3051099514178737	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8028	0.7306431443502572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.101185156331058E-5	0.0	0.0	0.006825888867248293	0.0
2	9.101185156331058E-5	0.0	0.0	0.023208022148644196	0.0
3	9.101185156331058E-5	0.0	0.0	0.039317119875350165	0.0
4	9.101185156331058E-5	0.0	0.0	0.05351496871922662	0.0
5	9.101185156331058E-5	0.0	0.0	0.09838381153993873	0.0
6	9.101185156331058E-5	0.0	0.0	0.1687359727983778	0.0
7	9.101185156331058E-5	0.0	0.0	0.2125126734003302	0.0
8	9.101185156331058E-5	0.0	0.0	0.29069185389321395	0.0
9	9.101185156331058E-5	0.0	0.0	0.433125401589795	0.0
10	9.101185156331058E-5	0.0	0.0	0.6031355403100592	0.0
11	9.101185156331058E-5	0.0	0.0	0.7713254419990572	0.0
12	9.101185156331058E-5	0.0	0.0	0.8771722253671873	0.0
13	9.101185156331058E-5	0.0	0.0	0.930414158531724	0.0
14	9.101185156331058E-5	0.0	0.0	0.9606300932507431	0.0
15	9.101185156331058E-5	0.0	0.0	0.9896628738994392	0.0
16	9.101185156331058E-5	0.0	0.0	1.029435053032606	0.0
17	9.101185156331058E-5	0.0	0.0	1.078126393618977	0.0
18	9.101185156331058E-5	0.0	0.0	1.1457481993305167	0.0
19	9.101185156331058E-5	0.0	0.0	1.1849743073543038	0.0
20	9.101185156331058E-5	0.0	0.0	1.2309352923937755	0.0
21	9.101185156331058E-5	0.0	0.0	1.2846322848161287	0.0
22	9.101185156331058E-5	0.0	0.0	1.3490686757229526	0.0
23	9.101185156331058E-5	0.0	0.0	1.4158713747704226	0.0
24	9.101185156331058E-5	0.0	0.0	1.4711155686693522	0.0
25	9.101185156331058E-5	0.0	0.0	1.5198979211072865	0.0
26	9.101185156331058E-5	0.0	0.0	1.5736859253812032	0.0
27	9.101185156331058E-5	0.0	0.0	1.6347548777801846	0.0
28	9.101185156331058E-5	0.0	0.0	1.698463173874502	0.0
29	1.8202370312662115E-4	0.0	0.0	1.7668130743985482	0.0
30	1.8202370312662115E-4	0.0	0.0	1.8449012430398686	0.0
31	1.8202370312662115E-4	0.0	0.0	1.9142522739311114	0.0
32	1.8202370312662115E-4	0.0	0.0	1.9864246722208165	0.0
33	1.8202370312662115E-4	0.0	0.0	2.063329686791814	0.0
34	1.8202370312662115E-4	0.0	0.0	2.1401436895112482	0.0
35	1.8202370312662115E-4	0.0	0.0	2.228334173676096	0.0
36	1.8202370312662115E-4	0.0	0.0	2.3137032904424815	0.0
37	1.8202370312662115E-4	0.0	0.0	2.4022578220135826	0.0
38	1.8202370312662115E-4	0.0	0.0	2.5208462646005763	0.0
39	1.8202370312662115E-4	0.0	0.0	2.6906743796177137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2118653E-7	45.000004	1
ATTCGCG	20	7.033149E-4	45.000004	1
CGTTTTT	10500	0.0	44.18572	1
AGCGATA	125	0.0	43.2	8
ACGGTCT	175	0.0	42.428574	30
GTTTTTA	7285	0.0	42.12766	2
TCACGAC	180	0.0	40.0	25
TTAGCGA	130	0.0	39.80769	2
GTTACGA	80	0.0	39.375004	2
TACGCGG	40	3.4580262E-7	39.375004	2
GTATGCG	40	3.4580262E-7	39.375004	1
CGAGGTA	75	0.0	39.0	6
GAGCGAT	715	0.0	38.706295	7
GCGATAA	70	0.0	38.57143	9
CACGACG	190	0.0	37.894737	26
CTCGTCA	30	1.13988935E-4	37.499996	21
TTATTTT	8485	0.0	37.442543	6
ATACGAG	115	0.0	37.173916	3
CGGTCTA	200	0.0	37.125	31
TTTTATT	8615	0.0	37.060356	4
>>END_MODULE