FastQCFastQC Report
Thu 26 May 2016
SRR1546946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546946_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1062997
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100710.9474156559237702No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56910.5353731007707453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT207.0330454E-445.00000411
TCACGAC1350.045.025
CGGTCTA1300.044.99999631
CGTTTTT81750.043.8990861
GAGCGAT7600.041.7434237
GTTTTTA57100.041.611212
CACGACG1450.040.34482626
AATGCGA850.039.7058832
CGACGGT1500.039.028
GAGGGGA63400.038.931397
AGCGATG3150.037.8571438
AGGGGAA64550.037.680098
CCGCTCG900.037.519
CGAGATC1500.037.56
TGATGCG601.546141E-1037.4999961
TAGTCGA301.1398643E-437.4999962
GGGCGAT5050.037.4257437
TATTTTT65900.037.3520477
TAGAGGC4900.037.193885
ATTAGCG850.037.0588231