##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546946_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1062997
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73739154484914	31.0	31.0	34.0	30.0	34.0
2	32.095606102369054	33.0	31.0	34.0	30.0	34.0
3	32.062771578847354	33.0	31.0	34.0	30.0	34.0
4	35.84266089179932	37.0	35.0	37.0	35.0	37.0
5	35.84362608737372	37.0	35.0	37.0	35.0	37.0
6	35.89015679254034	37.0	35.0	37.0	35.0	37.0
7	36.12235217973334	37.0	35.0	37.0	35.0	37.0
8	13.219526489726688	0.0	0.0	36.0	0.0	37.0
9	24.928196410714236	18.0	17.0	39.0	17.0	39.0
10	32.72611964097735	32.0	32.0	38.0	27.0	39.0
11	35.55482564861425	37.0	35.0	37.0	32.0	39.0
12	36.695518425734036	38.0	35.0	39.0	33.0	39.0
13	36.98188423861968	39.0	37.0	39.0	33.0	39.0
14	38.172916762700176	40.0	37.0	41.0	33.0	41.0
15	38.456850771921275	40.0	38.0	41.0	34.0	41.0
16	38.5290391224058	40.0	38.0	41.0	34.0	41.0
17	38.51495347587999	40.0	38.0	41.0	34.0	41.0
18	38.436928796600554	40.0	38.0	41.0	34.0	41.0
19	38.40143575193533	40.0	37.0	41.0	34.0	41.0
20	38.34161055957825	40.0	37.0	41.0	34.0	41.0
21	38.27384649251127	40.0	37.0	41.0	34.0	41.0
22	38.23642305669724	40.0	37.0	41.0	34.0	41.0
23	38.08283090168646	40.0	37.0	41.0	34.0	41.0
24	37.887237687406454	40.0	35.0	41.0	34.0	41.0
25	37.788601473005095	40.0	35.0	41.0	33.0	41.0
26	37.7161591236852	40.0	35.0	41.0	33.0	41.0
27	37.67605741126268	40.0	35.0	41.0	33.0	41.0
28	37.59767148919517	40.0	35.0	41.0	33.0	41.0
29	37.57162814194208	40.0	35.0	41.0	33.0	41.0
30	37.453858289346066	40.0	35.0	41.0	33.0	41.0
31	37.34846758739677	40.0	35.0	41.0	33.0	41.0
32	37.14202015621869	40.0	35.0	41.0	32.0	41.0
33	36.89439857309099	40.0	35.0	41.0	31.0	41.0
34	36.62154832045622	40.0	35.0	41.0	30.0	41.0
35	36.39923066574976	40.0	35.0	41.0	30.0	41.0
36	36.25050023659521	40.0	35.0	41.0	29.0	41.0
37	36.18084811151866	40.0	35.0	41.0	29.0	41.0
38	36.080592889725935	40.0	35.0	41.0	29.0	41.0
39	35.99657101572253	39.0	35.0	41.0	28.0	41.0
40	35.88377671809045	39.0	35.0	41.0	27.0	41.0
41	35.811377642646214	39.0	35.0	41.0	27.0	41.0
42	35.75939160693774	39.0	35.0	41.0	26.0	41.0
43	35.66719661485404	39.0	35.0	41.0	26.0	41.0
44	35.443307930314006	39.0	35.0	40.0	25.0	41.0
45	35.38141217708046	39.0	35.0	40.0	24.0	41.0
46	35.38194463389831	39.0	35.0	40.0	25.0	41.0
47	35.35451370041496	38.0	35.0	40.0	25.0	41.0
48	35.28277031826054	38.0	35.0	40.0	24.0	41.0
49	35.193637423247665	38.0	35.0	40.0	24.0	41.0
50	35.097843173593155	38.0	34.0	40.0	24.0	41.0
51	34.737437640934075	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	2.0
13	6.0
14	12.0
15	16.0
16	45.0
17	103.0
18	298.0
19	645.0
20	1205.0
21	2125.0
22	3387.0
23	5430.0
24	8737.0
25	13506.0
26	17809.0
27	19088.0
28	18186.0
29	18446.0
30	20764.0
31	25992.0
32	33338.0
33	45818.0
34	68513.0
35	103280.0
36	110637.0
37	190234.0
38	246611.0
39	108739.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13630894536861	22.31539693903181	27.525947862505728	18.022346253093847
2	35.27855675980271	23.833463311749703	28.718895725952194	12.169084202495398
3	28.366213639361167	23.981347078119693	34.702543845373036	12.949895437146106
4	25.80138984399768	25.928671482610017	33.77319032885323	14.496748344539073
5	22.400721732987016	30.44505299638663	32.84647087432984	14.30775439629651
6	20.757161120868638	38.82701456354063	30.32586169104899	10.089962624541744
7	83.02187118119807	4.092673826925194	11.05751004000952	1.8279449518672206
8	31.36584581141809	63.70892862350506	3.9838306222877393	0.9413949427891142
9	79.88216335511765	4.9465802819763365	12.048387718874089	3.122868644031921
10	42.24593296124072	28.92049554232044	17.679353751703907	11.154217744734932
11	32.81476805673017	23.209943207741883	28.09161267623521	15.883676059292737
12	30.074590991319827	22.06036329359349	31.3201260210518	16.544919694034885
13	23.057543906520902	28.09057786616519	31.3237008194755	17.528177407838406
14	17.766183723942778	31.041573964931228	33.591440051100804	17.60080226002519
15	17.343510847161376	24.95068189279932	41.15326760094337	16.552539659095935
16	21.186042858070152	22.58774013473227	38.65580053377385	17.570416473423727
17	21.019250289511636	22.17381610672467	31.32567636597281	25.48125723779089
18	22.468831050322812	24.419353958665923	34.03763133856445	19.07418365244681
19	25.083419802689942	25.690194798291998	29.780799005077153	19.445586393940907
20	26.230930096698295	24.855667513643027	29.79340487320284	19.119997516455832
21	24.317284056304956	25.199412604174796	32.35249017635986	18.130813163160386
22	23.12659396028399	22.171746486584627	32.59717572109799	22.10448383203339
23	20.588110784884623	26.305248274454208	31.87139756744375	21.235243373217422
24	20.68538293146641	25.1400521356128	35.548454040792215	18.626110892128576
25	20.87945685641634	25.18906450347461	33.55381059400921	20.37766804609985
26	19.26345982161756	28.771294744952243	30.66669049865616	21.29855493477404
27	19.033731986073338	27.78079336065859	33.46632210627123	19.719152546996842
28	17.893183141626928	26.563574497388043	36.15362978446788	19.38961257651715
29	18.806261917954615	26.053601280154133	35.5039572077814	19.636179594109862
30	20.313133527187755	26.305812716310584	34.09962586912287	19.2814278873788
31	25.106091550587635	24.507030593689354	30.779861090859146	19.60701676486387
32	24.654726212773884	26.49593554826589	30.40921093850688	18.44012730045334
33	24.133746379340675	25.681916317731847	29.6119368163786	20.57240048654888
34	20.25988784540314	26.954732703855232	31.3230423039764	21.462337146765233
35	20.764875159572416	26.971383738618265	31.456062434795207	20.807678667014113
36	25.103645635876674	26.081823372972828	29.617675308585067	19.196855682565424
37	21.531575347813774	28.98550042944618	29.362453515861286	20.12047070687876
38	21.12329573836991	30.25878718378321	27.710802570468214	20.907114507378665
39	21.53213978967015	28.271105186562146	28.463203565014766	21.73355145875294
40	22.89592538831248	26.149274174809523	29.01757954161677	21.937220895261227
41	20.030160009858918	25.816253479548863	29.83677282250091	24.31681368809131
42	21.997333952965057	27.474207359004776	27.99659829707892	22.531860390951245
43	21.44098242986575	26.74984030999147	28.548340211684508	23.260837048458274
44	20.63749944731735	28.326326414844065	29.491334406400014	21.544839731438564
45	20.618026203272446	30.130094440529938	27.5417522344842	21.71012712171342
46	22.291314086493188	28.017388572122027	28.917014817539467	20.774282523845315
47	21.358950213406057	27.785497042795043	29.579575483279818	21.27597726051908
48	21.34182881042938	26.902804053068824	30.622758107501713	21.132609029000083
49	21.4875488830166	26.180224403267367	30.467348449713406	21.864878264002627
50	19.973809897864246	28.135545067389657	30.496981647173037	21.39366338757306
51	19.77098712414052	29.30092935351652	29.65125959903932	21.27682392330364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	730.0
1	1351.0
2	1972.0
3	13848.5
4	25725.0
5	17485.0
6	9245.0
7	9103.5
8	8962.0
9	8906.5
10	8851.0
11	8654.5
12	8458.0
13	8093.0
14	7728.0
15	7318.0
16	6908.0
17	6341.0
18	5774.0
19	5727.5
20	5681.0
21	6024.0
22	6367.0
23	7009.5
24	7652.0
25	8579.5
26	10886.5
27	12266.0
28	14679.0
29	17092.0
30	19633.0
31	22174.0
32	24516.5
33	26859.0
34	30090.0
35	33321.0
36	36326.5
37	39332.0
38	45422.0
39	51512.0
40	59361.5
41	67211.0
42	72034.5
43	76858.0
44	78004.0
45	79150.0
46	80382.0
47	81614.0
48	80178.0
49	78742.0
50	74642.5
51	70543.0
52	65994.5
53	61446.0
54	57523.5
55	53601.0
56	50015.5
57	46430.0
58	42515.0
59	38600.0
60	34359.5
61	30119.0
62	26434.5
63	22750.0
64	19053.0
65	15356.0
66	12622.5
67	9889.0
68	7967.5
69	6046.0
70	4937.5
71	3829.0
72	3037.0
73	2245.0
74	1741.5
75	961.5
76	685.0
77	491.5
78	298.0
79	212.5
80	127.0
81	89.0
82	51.0
83	37.5
84	24.0
85	17.0
86	10.0
87	10.0
88	10.0
89	6.5
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1062997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.5415493896331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69429291089804	21.982659820246468
2	6.871826595953164	4.33496115946502
3	3.9655995445685126	3.752434616835428
4	2.7252788709499254	3.4383807243436237
5	2.237513089167023	3.528731480595609
6	1.8374413172849293	3.4773447635817063
7	1.58812825516937	3.506441805824757
8	1.4060221569928377	3.547849384616645
9	1.1934333754950737	3.3878463980772975
>10	8.396612705159217	44.864755051259586
>50	0.059979727981800936	1.178965873045346
>100	0.022081091605091814	1.260896385345304
>500	8.951793875382246E-4	0.15962381630130945
>1k	2.983931291794082E-4	0.09562370127484104
>5k	2.983931291794082E-4	0.5356244920818114
>10k+	2.983931291794082E-4	0.9478605271052404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10071	0.9474156559237702	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5691	0.5353731007707453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.407364272900112E-5	0.0	0.0	0.009877732486545117	0.0
2	9.407364272900112E-5	0.0	0.0	0.027187282748681322	0.0
3	9.407364272900112E-5	0.0	0.0	0.042521286513508505	0.0
4	9.407364272900112E-5	0.0	0.0	0.06839153826398381	0.0
5	9.407364272900112E-5	0.0	0.0	0.1265290494705065	0.0
6	9.407364272900112E-5	0.0	0.0	0.20489239386376443	0.0
7	9.407364272900112E-5	0.0	0.0	0.25757363379200504	0.0
8	9.407364272900112E-5	0.0	0.0	0.3480724780973041	0.0
9	9.407364272900112E-5	0.0	0.0	0.49049997318901184	0.0
10	9.407364272900112E-5	0.0	0.0	0.6984967972628333	0.0
11	9.407364272900112E-5	0.0	0.0	0.8902188811445376	0.0
12	9.407364272900112E-5	0.0	0.0	1.0461929807892214	0.0
13	9.407364272900112E-5	0.0	0.0	1.1208874531160482	0.0
14	9.407364272900112E-5	0.0	0.0	1.1540013753566567	0.0
15	9.407364272900112E-5	0.0	0.0	1.1967108091556231	0.0
16	9.407364272900112E-5	0.0	0.0	1.274509711692507	0.0
17	9.407364272900112E-5	0.0	0.0	1.3685833544215082	0.0
18	9.407364272900112E-5	0.0	0.0	1.50969381851501	0.0
19	9.407364272900112E-5	0.0	0.0	1.5751690738543946	0.0
20	9.407364272900112E-5	0.0	0.0	1.6544731546749427	0.0
21	9.407364272900112E-5	0.0	0.0	1.7465712509066347	0.0
22	9.407364272900112E-5	0.0	0.0	1.847230048626666	0.0
23	9.407364272900112E-5	0.0	0.0	1.9578606524759712	0.0
24	9.407364272900112E-5	0.0	0.0	2.042903225502988	0.0
25	9.407364272900112E-5	0.0	0.0	2.117597697829815	0.0
26	9.407364272900112E-5	0.0	0.0	2.192104022871184	0.0
27	9.407364272900112E-5	0.0	0.0	2.278745847824594	0.0
28	9.407364272900112E-5	0.0	0.0	2.367269145632584	0.0
29	9.407364272900112E-5	0.0	0.0	2.4643531449289133	0.0
30	9.407364272900112E-5	0.0	0.0	2.5719733922108907	0.0
31	9.407364272900112E-5	0.0	0.0	2.670750717076342	0.0
32	9.407364272900112E-5	0.0	0.0	2.769057673728148	0.0
33	9.407364272900112E-5	0.0	0.0	2.873103122586423	0.0
34	9.407364272900112E-5	0.0	0.0	2.981475959010232	0.0
35	9.407364272900112E-5	0.0	0.0	3.101984295346083	0.0
36	9.407364272900112E-5	0.0	0.0	3.205371228705255	0.0
37	9.407364272900112E-5	0.0	0.0	3.3261617859692927	0.0
38	9.407364272900112E-5	0.0	0.0	3.457676738504436	0.0
39	9.407364272900112E-5	0.0	0.0	3.6438484774651294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGT	20	7.0330454E-4	45.000004	11
TCACGAC	135	0.0	45.0	25
CGGTCTA	130	0.0	44.999996	31
CGTTTTT	8175	0.0	43.899086	1
GAGCGAT	760	0.0	41.743423	7
GTTTTTA	5710	0.0	41.61121	2
CACGACG	145	0.0	40.344826	26
AATGCGA	85	0.0	39.705883	2
CGACGGT	150	0.0	39.0	28
GAGGGGA	6340	0.0	38.93139	7
AGCGATG	315	0.0	37.857143	8
AGGGGAA	6455	0.0	37.68009	8
CCGCTCG	90	0.0	37.5	19
CGAGATC	150	0.0	37.5	6
TGATGCG	60	1.546141E-10	37.499996	1
TAGTCGA	30	1.1398643E-4	37.499996	2
GGGCGAT	505	0.0	37.425743	7
TATTTTT	6590	0.0	37.352047	7
TAGAGGC	490	0.0	37.19388	5
ATTAGCG	85	0.0	37.058823	1
>>END_MODULE