FastQCFastQC Report
Thu 26 May 2016
SRR1546945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546945_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1216279
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37060.30469982627341263No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.23769217424620503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16550.1360707535031025No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.13549522765747005No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTG302.165607E-645.00000437
GTCGATC302.165607E-645.00000418
TTAGTCG302.165607E-645.00000414
TATAGCG302.165607E-645.0000041
ACGTATA302.165607E-645.00000431
CCGAACC302.165607E-645.00000414
CACGTAA302.165607E-645.00000433
CGCGTCA600.045.00000439
GCGCTCA302.165607E-645.00000435
ACGAGCC302.165607E-645.0000045
TGTCGGA600.045.0000042
CGCTTTA302.165607E-645.00000416
AAATGCG302.165607E-645.0000041
CTTCGTC207.033448E-445.041
ACAACGC207.033448E-445.027
TATCACG502.1827873E-1145.01
CGGAATA351.2120108E-745.05
CTATGCG406.8157533E-945.01
GTACCGC253.8909402E-545.045
CGAACCG207.033448E-445.06