##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546945_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1216279
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805616967817418	31.0	31.0	34.0	30.0	34.0
2	32.1386466427522	33.0	31.0	34.0	30.0	34.0
3	32.148026891856226	33.0	31.0	34.0	30.0	34.0
4	35.86238025979237	37.0	35.0	37.0	35.0	37.0
5	35.82558935902042	37.0	35.0	37.0	35.0	37.0
6	35.8756099546239	37.0	35.0	37.0	35.0	37.0
7	36.13392897517757	37.0	35.0	37.0	35.0	37.0
8	13.295272712921953	0.0	0.0	37.0	0.0	37.0
9	25.0215468654807	18.0	17.0	39.0	17.0	39.0
10	32.844155000620745	32.0	32.0	38.0	27.0	39.0
11	35.74947195503663	37.0	35.0	37.0	32.0	39.0
12	36.435131248669094	37.0	35.0	39.0	33.0	39.0
13	36.272546841637485	38.0	35.0	39.0	32.0	39.0
14	37.35550478138651	39.0	35.0	41.0	33.0	41.0
15	37.85292519232841	40.0	36.0	41.0	33.0	41.0
16	38.078220539859686	40.0	36.0	41.0	34.0	41.0
17	38.11599558982766	40.0	36.0	41.0	34.0	41.0
18	38.064235261810815	40.0	36.0	41.0	34.0	41.0
19	38.00435179757276	40.0	36.0	41.0	34.0	41.0
20	37.97835365076598	40.0	36.0	41.0	34.0	41.0
21	37.85695716196695	40.0	35.0	41.0	34.0	41.0
22	37.7846596052386	40.0	35.0	41.0	33.0	41.0
23	37.73009399981419	39.0	35.0	41.0	33.0	41.0
24	37.6706602679155	40.0	35.0	41.0	33.0	41.0
25	37.56133502263872	39.0	35.0	41.0	33.0	41.0
26	37.489441978361874	39.0	35.0	41.0	33.0	41.0
27	37.3690806139052	39.0	35.0	41.0	33.0	41.0
28	37.40429704039945	39.0	35.0	41.0	33.0	41.0
29	37.51462534500719	40.0	35.0	41.0	33.0	41.0
30	37.45788014098739	40.0	35.0	41.0	33.0	41.0
31	37.32707791551116	40.0	35.0	41.0	33.0	41.0
32	37.132322435888476	39.0	35.0	41.0	33.0	41.0
33	37.0214745136601	39.0	35.0	41.0	32.0	41.0
34	36.94050871551675	40.0	35.0	41.0	32.0	41.0
35	36.86277243954718	40.0	35.0	41.0	32.0	41.0
36	36.73338025239275	39.0	35.0	41.0	31.0	41.0
37	36.60514240564871	39.0	35.0	41.0	31.0	41.0
38	36.5422842949685	39.0	35.0	41.0	31.0	41.0
39	36.53567314736175	39.0	35.0	41.0	31.0	41.0
40	36.400344000019736	39.0	35.0	41.0	31.0	41.0
41	36.40464235590683	39.0	35.0	41.0	31.0	41.0
42	36.36461617770265	39.0	35.0	41.0	31.0	41.0
43	36.266899288732276	39.0	35.0	41.0	30.0	41.0
44	36.02982210496111	38.0	35.0	40.0	30.0	41.0
45	35.95732886944525	38.0	35.0	40.0	30.0	41.0
46	35.98477076394479	38.0	35.0	40.0	30.0	41.0
47	35.97619131794597	38.0	35.0	40.0	30.0	41.0
48	35.95754263618792	38.0	35.0	40.0	30.0	41.0
49	35.917486859511676	38.0	35.0	40.0	30.0	41.0
50	35.78120809452437	38.0	35.0	40.0	29.0	41.0
51	35.30690820116108	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	3.0
14	4.0
15	14.0
16	54.0
17	121.0
18	310.0
19	700.0
20	1196.0
21	2119.0
22	3219.0
23	5075.0
24	7417.0
25	10561.0
26	13213.0
27	14796.0
28	15800.0
29	18760.0
30	23132.0
31	30084.0
32	41078.0
33	57842.0
34	98800.0
35	169330.0
36	113105.0
37	197391.0
38	303080.0
39	89053.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.909684373404456	24.01661132026451	31.297342139426892	13.776362166904141
2	28.812714845853627	26.35678162658403	32.325066863770566	12.505436663791777
3	27.719544611063746	26.04969747895014	32.61562519783701	13.615132712149103
4	25.265337969331046	27.734919372939927	32.19672460019453	14.803018057534498
5	23.416091209336017	32.54458886489037	30.48774171057792	13.551578215195692
6	21.704888434314825	40.69625472445056	26.7450149184521	10.85384192278252
7	88.11152704272621	4.800214424486486	5.6986102695187535	1.3896482632685427
8	33.85062144458632	63.499739780099794	1.8083022069771821	0.8413365683367057
9	87.27463024519868	4.751212509629781	5.793489816070162	2.180667429101382
10	59.25704546407526	23.017251798312724	9.753272069977365	7.972430667634646
11	57.812146719626014	16.798366164342227	16.327914894526668	9.061572221505099
12	50.44558033148644	20.81964746575416	17.72570273761201	11.009069465147387
13	20.32971053516504	50.44188052247881	18.86310624453764	10.36530269781851
14	12.066803751441897	52.605528830145055	24.858934504336588	10.468732914076458
15	9.487214693339276	24.281188773299547	55.697335890860565	10.53426064250061
16	11.682270268581469	16.968639596671487	55.02298403573522	16.326106099011824
17	13.58832965133822	16.73760707863903	27.65311248488217	42.020950785140585
18	20.362022200498405	22.96381011264685	35.667474321270035	21.006693365584706
19	29.42301889615787	24.828842724407803	25.339251931505846	20.408886447928477
20	33.45120650771739	22.954930571028523	23.681737496084367	19.91212542516972
21	21.78562648865926	28.67458864290183	28.62764217749382	20.91214269094509
22	21.955653267054682	24.632259539135344	26.17318888182728	27.238898311982695
23	16.874911101811342	31.774124193544406	24.602085541228615	26.74887916341563
24	18.600173151061558	24.2843952744395	41.11614193782841	15.999289636670532
25	13.28428756888839	25.10468404042165	40.378728893617335	21.232299497072628
26	13.001622160704903	38.70633300418736	27.76961535963377	20.522429475473967
27	14.936046745853544	39.00092001917323	30.246760817213815	15.81627241775941
28	10.460182244369918	31.316992236156345	43.987769253600526	14.235056265873208
29	11.31582474086949	24.479662972064798	43.28258565674488	20.92192663032084
30	15.12407926141946	32.70910703876331	34.34590254374202	17.82091115607521
31	30.558695825546607	27.89286010857706	23.8183837754331	17.730060290443227
32	31.136934864451334	28.460081938436822	26.34009137706069	14.062891820051156
33	27.4225732747174	29.440202453548896	24.585806381595013	18.551417890138694
34	17.169580334775162	31.10388323731644	27.426108647769137	24.300427780139263
35	16.92596846611674	26.46086958666556	32.52666534569782	24.086496601519883
36	32.40243398101916	21.76901845711387	28.544355365833002	17.284192196033967
37	19.224372039638933	32.03343969599081	31.55320448679949	17.188983777570773
38	17.95204883090146	34.88475917120989	23.728108435646757	23.435083562241886
39	18.3494083183217	32.344388088588225	29.63431909948293	19.67188449360714
40	22.69290187530986	26.96453691957191	24.702144820390718	25.640416384727516
41	15.543555384907576	23.008043384782603	29.023192869399207	32.42520836091061
42	22.56233972632924	24.974779635264607	24.81124807712704	27.651632561279115
43	23.163599799059263	25.664177380354342	25.482722303024225	25.68950051756217
44	18.06509855057927	33.09117398228531	27.907823780563508	20.935903686571912
45	15.19791100561631	41.587826477313186	21.52425553676418	21.69000698030633
46	22.662234569535443	34.847514427199684	24.878995690955776	17.6112553123091
47	21.376756484326375	29.40599977472274	25.98655407188647	23.230689669064418
48	24.19650425601363	23.815095056315204	30.20038987765143	21.78801081001974
49	20.974217264295444	20.963364491206377	33.32607074528131	24.736347499216873
50	19.126368209925516	33.916231390988415	26.685078012528372	20.27232238655769
51	16.807245705960558	37.35861590967204	22.767473581308238	23.06666480305917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1954.0
1	1895.5
2	1837.0
3	4595.5
4	7354.0
5	5547.5
6	3741.0
7	4342.0
8	4943.0
9	5496.5
10	6050.0
11	6261.0
12	6472.0
13	6234.0
14	5996.0
15	5626.0
16	5256.0
17	5002.0
18	4748.0
19	4705.5
20	4663.0
21	5068.5
22	5474.0
23	5825.5
24	6177.0
25	7285.5
26	9592.0
27	10790.0
28	13023.0
29	15256.0
30	17356.5
31	19457.0
32	21981.5
33	24506.0
34	26297.0
35	28088.0
36	34012.0
37	39936.0
38	60136.5
39	80337.0
40	106636.0
41	132935.0
42	145964.0
43	158993.0
44	152443.0
45	145893.0
46	134423.0
47	122953.0
48	110488.0
49	98023.0
50	87135.5
51	76248.0
52	67916.5
53	59585.0
54	51496.5
55	43408.0
56	37653.0
57	31898.0
58	26921.0
59	21944.0
60	17933.5
61	13923.0
62	11087.5
63	8252.0
64	6659.0
65	5066.0
66	3902.5
67	2739.0
68	2114.5
69	1490.0
70	1108.0
71	726.0
72	572.0
73	418.0
74	273.5
75	121.0
76	113.0
77	91.5
78	70.0
79	45.0
80	20.0
81	15.5
82	11.0
83	7.5
84	4.0
85	3.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1216279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.914949964054706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2813639801613	15.987993467109762
2	6.68746691271919	2.663611379061481
3	2.1315068695527972	1.273465579655485
4	1.0313821712364808	0.8215969733597048
5	0.6332563602182494	0.6305634364082919
6	0.45587207901381865	0.5447201786141875
7	0.3696352649386203	0.5152887464341911
8	0.28846232368917085	0.45957701942278323
9	0.24996242836059818	0.44801903283254296
>10	4.905045872355947	26.518242230334206
>50	2.3202400441236697	32.29997887656812
>100	0.6437251340501982	16.97353638847918
>500	4.1611191599883526E-4	0.04300874105026581
>1k	0.001664447663995341	0.8203979506698102
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3706	0.30469982627341263	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2891	0.23769217424620503	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1655	0.1360707535031025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1648	0.13549522765747005	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.221797794749396E-5	0.0	0.0	0.01825239110434366	0.0
2	8.221797794749396E-5	0.0	0.0	0.06347227897546533	0.0
3	8.221797794749396E-5	0.0	0.0	0.09191969934529824	0.0
4	8.221797794749396E-5	0.0	0.0	0.16501148174062036	0.0
5	8.221797794749396E-5	0.0	0.0	0.2852141654998565	0.0
6	8.221797794749396E-5	0.0	0.0	0.4115009796272072	0.0
7	8.221797794749396E-5	0.0	0.0	0.48607268562558426	0.0
8	8.221797794749396E-5	0.0	0.0	0.6651434415952261	0.0
9	8.221797794749396E-5	0.0	0.0	0.8250574087031018	0.0
10	8.221797794749396E-5	0.0	0.0	1.1444742530291159	0.0
11	8.221797794749396E-5	0.0	0.0	1.4663576366935547	0.0
12	8.221797794749396E-5	0.0	0.0	1.7643978067532202	0.0
13	8.221797794749396E-5	0.0	0.0	1.8744876792249148	0.0
14	8.221797794749396E-5	0.0	0.0	1.9091014479408097	0.0
15	8.221797794749396E-5	0.0	0.0	1.9740536505193298	0.0
16	8.221797794749396E-5	0.0	0.0	2.120401651265869	0.0
17	8.221797794749396E-5	0.0	0.0	2.2919083532643416	0.0
18	8.221797794749396E-5	0.0	0.0	2.572189440087348	0.0
19	8.221797794749396E-5	0.0	0.0	2.683923672117993	0.0
20	8.221797794749396E-5	0.0	0.0	2.8032219581198063	0.0
21	8.221797794749396E-5	0.0	0.0	2.9683978758163216	0.0
22	8.221797794749396E-5	0.0	0.0	3.1269963552770377	0.0
23	8.221797794749396E-5	0.0	0.0	3.300969596613935	0.0
24	8.221797794749396E-5	0.0	0.0	3.4186235230567985	0.0
25	8.221797794749396E-5	0.0	0.0	3.5164629168143167	0.0
26	8.221797794749396E-5	0.0	0.0	3.6235107241019535	0.0
27	8.221797794749396E-5	0.0	0.0	3.720445720102049	0.0
28	8.221797794749396E-5	0.0	0.0	3.8222315768010464	0.0
29	8.221797794749396E-5	0.0	0.0	3.9342946807434807	0.0
30	8.221797794749396E-5	0.0	0.0	4.096099661344149	0.0
31	0.001562141581002385	0.0	0.0	4.257329116099185	0.0
32	0.001562141581002385	0.0	0.0	4.380409429086583	0.0
33	0.001562141581002385	0.0	0.0	4.501598728581189	0.0
34	0.001562141581002385	0.0	0.0	4.642684778739088	0.0
35	0.001562141581002385	0.0	0.0	4.83096394823885	0.0
36	0.001562141581002385	0.0	0.0	4.979449616412024	0.0
37	0.001562141581002385	0.0	0.0	5.124646565467298	0.0
38	0.001562141581002385	0.0	0.0	5.257099727940711	0.0
39	0.001562141581002385	0.0	0.0	5.391443903906916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTG	30	2.165607E-6	45.000004	37
GTCGATC	30	2.165607E-6	45.000004	18
TTAGTCG	30	2.165607E-6	45.000004	14
TATAGCG	30	2.165607E-6	45.000004	1
ACGTATA	30	2.165607E-6	45.000004	31
CCGAACC	30	2.165607E-6	45.000004	14
CACGTAA	30	2.165607E-6	45.000004	33
CGCGTCA	60	0.0	45.000004	39
GCGCTCA	30	2.165607E-6	45.000004	35
ACGAGCC	30	2.165607E-6	45.000004	5
TGTCGGA	60	0.0	45.000004	2
CGCTTTA	30	2.165607E-6	45.000004	16
AAATGCG	30	2.165607E-6	45.000004	1
CTTCGTC	20	7.033448E-4	45.0	41
ACAACGC	20	7.033448E-4	45.0	27
TATCACG	50	2.1827873E-11	45.0	1
CGGAATA	35	1.2120108E-7	45.0	5
CTATGCG	40	6.8157533E-9	45.0	1
GTACCGC	25	3.8909402E-5	45.0	45
CGAACCG	20	7.033448E-4	45.0	6
>>END_MODULE