FastQCFastQC Report
Thu 26 May 2016
SRR1546944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546944_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1161033
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151841.3078008979934248No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84060.7240104286441471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121800.043.891631
CGGTCTA2000.042.75000431
GTTTTTA86450.041.6946222
CGTTAGA1050.040.7142872
CACGACG2050.040.60975326
ACGGTCT2150.039.7674430
TATACGA850.039.7058832
TGTACGA1500.039.02
CGACGGT2200.038.86363628
TTATTTT95250.038.8582656
TATTTTT95350.038.8411147
CGACGTT1450.038.79310227
TCGACGT1450.038.79310226
ACGGGTG356.248343E-638.5714265
TTTTATT96750.038.348844
TTTATTT97150.038.1214645
TTTTTAT97450.037.8424843
ACGACGG2200.037.84090827
CGCGCGA301.1399289E-437.5000042
TTATACG301.1399289E-437.5000041
Produced by FastQC (version 0.11.5)