##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546944_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1161033
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68543012989295	31.0	31.0	34.0	30.0	34.0
2	32.03324367179916	31.0	31.0	34.0	30.0	34.0
3	31.966005272890605	33.0	31.0	34.0	30.0	34.0
4	35.784021642795686	37.0	35.0	37.0	35.0	37.0
5	35.802641268594435	37.0	35.0	37.0	35.0	37.0
6	35.8537250879174	37.0	35.0	37.0	35.0	37.0
7	36.08475469689492	37.0	35.0	37.0	35.0	37.0
8	13.281868818543487	0.0	0.0	35.0	0.0	37.0
9	24.949606083548012	18.0	17.0	39.0	17.0	39.0
10	32.66204578164445	32.0	32.0	38.0	27.0	39.0
11	35.577410805722145	37.0	35.0	37.0	32.0	39.0
12	36.670449504880565	38.0	35.0	39.0	33.0	39.0
13	36.93048776391369	39.0	37.0	39.0	33.0	39.0
14	38.069351172619555	40.0	37.0	41.0	33.0	41.0
15	38.31658617799839	40.0	37.0	41.0	33.0	41.0
16	38.38164634424689	40.0	38.0	41.0	34.0	41.0
17	38.33013187394329	40.0	37.0	41.0	33.0	41.0
18	38.21200775516286	40.0	37.0	41.0	33.0	41.0
19	38.17593815162876	40.0	37.0	41.0	34.0	41.0
20	38.11835408640409	40.0	36.0	41.0	34.0	41.0
21	38.067163465637925	40.0	36.0	41.0	33.0	41.0
22	38.03467084914899	40.0	36.0	41.0	33.0	41.0
23	37.872722825277144	39.0	36.0	41.0	33.0	41.0
24	37.6163278735402	39.0	35.0	41.0	33.0	41.0
25	37.493930835729905	39.0	35.0	41.0	33.0	41.0
26	37.424835469792846	39.0	35.0	41.0	33.0	41.0
27	37.40251913597633	39.0	35.0	41.0	33.0	41.0
28	37.287277794860266	39.0	35.0	41.0	33.0	41.0
29	37.232129491582064	39.0	35.0	41.0	33.0	41.0
30	37.061333312662086	39.0	35.0	41.0	32.0	41.0
31	36.88645714635157	39.0	35.0	41.0	32.0	41.0
32	36.624173473105415	39.0	35.0	41.0	31.0	41.0
33	36.292504175161255	39.0	35.0	41.0	30.0	41.0
34	35.908732998975914	39.0	35.0	41.0	27.0	41.0
35	35.630774491336595	39.0	35.0	41.0	25.0	41.0
36	35.50698214434904	39.0	35.0	41.0	24.0	41.0
37	35.465666350568846	39.0	35.0	41.0	24.0	41.0
38	35.35994325742679	39.0	35.0	41.0	23.0	41.0
39	35.246319441394	39.0	35.0	41.0	23.0	41.0
40	35.154317749796945	39.0	35.0	41.0	23.0	41.0
41	35.06370792216931	39.0	35.0	40.0	23.0	41.0
42	35.00648215856052	39.0	35.0	40.0	23.0	41.0
43	34.91026180995717	38.0	34.0	40.0	22.0	41.0
44	34.741766168575744	38.0	34.0	40.0	21.0	41.0
45	34.6663367880155	38.0	34.0	40.0	22.0	41.0
46	34.65336299657288	38.0	34.0	40.0	22.0	41.0
47	34.61993069964419	38.0	34.0	40.0	22.0	41.0
48	34.539162969528	38.0	34.0	40.0	22.0	41.0
49	34.425889703393445	38.0	34.0	40.0	20.0	41.0
50	34.343687044209766	38.0	34.0	40.0	20.0	41.0
51	34.01719675495873	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	5.0
14	15.0
15	36.0
16	60.0
17	166.0
18	396.0
19	917.0
20	1779.0
21	2928.0
22	4763.0
23	7435.0
24	11864.0
25	18249.0
26	24371.0
27	25628.0
28	24528.0
29	24286.0
30	26087.0
31	30876.0
32	39318.0
33	53556.0
34	79215.0
35	112827.0
36	120802.0
37	200440.0
38	243619.0
39	106833.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.832256275230762	21.78818345387254	26.337063632127595	20.042496638769098
2	38.15481558233056	22.540013935865733	27.47157057551336	11.833599906290347
3	28.23709575869075	22.576963790004246	36.692755503073556	12.493184948231445
4	25.139853905961328	24.717385293958053	36.141436117664185	14.001324682416435
5	22.12012922974627	28.978418356756443	34.97833394916424	13.923118464333056
6	19.90839192339925	37.793154888793	32.74790639025764	9.550546797550112
7	80.35973137714431	3.8244390986302714	13.832337237615125	1.9834922866102858
8	30.587761071390734	63.40009284835143	5.057565116581527	0.9545809636763124
9	77.2586997957853	4.364303167954744	15.085876112048494	3.291120924211457
10	41.30976466646512	24.026276600234446	21.60188383964969	13.06207489365074
11	35.03276823311655	22.22159060078396	27.373037631143987	15.372603534955509
12	32.043447516134336	19.548884484764862	32.303302317849706	16.104365681251092
13	25.225381190715506	23.02923344986749	33.16675753402358	18.578627825393422
14	19.3746430979998	26.006409809195773	35.695798482902724	18.9231486099017
15	19.519944738866165	22.50633702918005	41.44438616301173	16.529332068942054
16	24.908938850144654	20.08065231565339	38.35386246558022	16.656546368621736
17	24.008016998655506	20.831449235293054	33.12584569086322	22.034688075188217
18	25.070346837686785	22.655256138283754	34.75637643374478	17.518020590284685
19	26.050852990397345	25.55095333207583	30.834868604079297	17.563325073447526
20	27.301549568358524	25.0417516125726	30.31007731907706	17.34662149999182
21	25.704092820789764	24.474584271075845	32.95005396056787	16.87126894756652
22	22.97566046787645	21.893951334716586	34.75155314276166	20.378835054645304
23	22.93896900432632	23.50596408543082	33.82996004420202	19.725106866040846
24	23.78778208715859	22.75060226539642	34.87204928714343	18.58956636030156
25	23.314841180224853	22.2035893897934	33.277865487027505	21.20370394295425
26	19.788929341371002	25.419001871609158	32.850056802864344	21.94201198415549
27	19.390146533302673	24.043674899852114	35.68804676525129	20.878131801593923
28	18.79274749296532	25.19954213187739	36.203966640052435	19.80374373510486
29	21.593959861606002	25.746124356499774	34.046922008246106	18.612993773648125
30	23.584514824298704	22.97109556748172	34.74078686824578	18.7036027399738
31	26.45979916160867	22.19204794351237	32.050510192216755	19.2976427026622
32	27.615580263437817	23.760392684790183	31.208070743897892	17.415956307874108
33	26.31492817172294	23.7986344918706	30.879225655084742	19.00721168132172
34	21.926853069637126	24.808424911264364	33.53375829972102	19.73096371937749
35	20.986311327929524	26.827833489659643	32.27918586293413	19.90666931947671
36	24.411623097706954	28.01307111856424	28.713568003665703	18.8617377800631
37	23.2096762107537	29.094091210155092	28.438984938412602	19.257247640678603
38	23.237410133906618	29.25300142200954	27.03730212664067	20.47228631744317
39	22.692722773599026	27.19121678711975	27.918155642432215	22.197904796849013
40	23.39132479438569	24.659075151180026	29.010631050107964	22.93896900432632
41	20.248778458493426	25.226759273853546	29.620863489668253	24.90359877798478
42	21.201464557854948	26.679603422124952	28.276887909301458	23.842044110718643
43	20.98734489028305	25.26655142446425	28.93578390967354	24.810319775579163
44	21.10603230054615	25.653706656055427	30.077439659337845	23.16282138406057
45	21.769062550332333	26.70613152253209	28.595569634971618	22.92923629216396
46	22.16724244702778	27.212060294582496	29.38925939228256	21.231437866107164
47	21.442370716422356	26.144907164568103	30.45744608465048	21.95527603435906
48	21.399305618358824	25.15656316400998	31.466891983259735	21.97723923437146
49	21.913847410021937	23.96477964019972	32.083239666745044	22.038133283033297
50	20.21225925533555	25.19204880481433	31.985568024336946	22.610123915513167
51	19.738284786048286	26.24137298423042	31.72536870183707	22.29497352788422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	695.0
1	1624.0
2	2553.0
3	21346.0
4	40139.0
5	26746.5
6	13354.0
7	12786.5
8	12219.0
9	12005.0
10	11791.0
11	11415.0
12	11039.0
13	10506.5
14	9974.0
15	9326.5
16	8679.0
17	8095.5
18	7512.0
19	7116.0
20	6720.0
21	6811.5
22	6903.0
23	7223.0
24	7543.0
25	8103.0
26	10240.0
27	11817.0
28	13339.0
29	14861.0
30	16483.5
31	18106.0
32	20159.0
33	22212.0
34	24524.0
35	26836.0
36	30245.0
37	33654.0
38	38671.0
39	43688.0
40	49843.5
41	55999.0
42	61088.0
43	66177.0
44	69129.0
45	72081.0
46	76267.5
47	80454.0
48	81196.0
49	81938.0
50	79425.0
51	76912.0
52	73825.5
53	70739.0
54	68910.0
55	67081.0
56	66008.5
57	64936.0
58	62747.0
59	60558.0
60	55576.5
61	50595.0
62	43786.5
63	36978.0
64	30195.5
65	23413.0
66	18612.0
67	13811.0
68	11173.5
69	8536.0
70	7023.5
71	5511.0
72	4410.5
73	3310.0
74	2432.5
75	1181.5
76	808.0
77	576.0
78	344.0
79	266.0
80	188.0
81	135.5
82	83.0
83	58.5
84	34.0
85	24.5
86	15.0
87	12.0
88	9.0
89	6.0
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1161033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.160512372939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.09000290299727	23.94079389544288
2	7.937183832032829	5.581509006649633
3	4.592556431885083	4.844299117401482
4	3.3776544102684145	4.750402387350182
5	2.711998104282069	4.76776214504984
6	2.199362362637413	4.639842453845346
7	1.856153280865757	4.568431027856623
8	1.520078560121398	4.275739281679017
9	1.2618744768764707	3.993133784157997
>10	6.396146252192819	33.98649061553202
>50	0.03171862541031155	0.7752411749956655
>100	0.023536492715396387	1.5512738287095786
>500	0.001238762653461567	0.2701313946050634
>1k	0.0	0.0
>5k	2.477525306923134E-4	0.7322555637053089
>10k+	2.477525306923134E-4	1.3226943230194397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15184	1.3078008979934248	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8406	0.7240104286441471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.722603922541392E-4	0.0	0.0	0.007665587455309195	0.0
2	1.722603922541392E-4	0.0	0.0	0.0215325490317674	0.0
3	1.722603922541392E-4	0.0	0.0	0.03634694276562337	0.0
4	1.722603922541392E-4	0.0	0.0	0.05443428395230799	0.0
5	1.722603922541392E-4	0.0	0.0	0.1025810635873399	0.0
6	1.722603922541392E-4	0.0	0.0	0.1991330134457849	0.0
7	1.722603922541392E-4	0.0	0.0	0.26958751387772784	0.0
8	1.722603922541392E-4	0.0	0.0	0.39034204884787943	0.0
9	1.722603922541392E-4	0.0	0.0	0.6522639752702981	0.0
10	1.722603922541392E-4	0.0	0.0	0.9734434766281407	0.0
11	1.722603922541392E-4	0.0	0.0	1.2055643551905932	0.0
12	1.722603922541392E-4	0.0	0.0	1.3680920352823736	0.0
13	1.722603922541392E-4	0.0	0.0	1.4635242925911667	0.0
14	1.722603922541392E-4	0.0	0.0	1.524590601645259	0.0
15	1.722603922541392E-4	0.0	0.0	1.5698950848080977	0.0
16	1.722603922541392E-4	0.0	0.0	1.6295833107241569	0.0
17	1.722603922541392E-4	0.0	0.0	1.694180957819459	0.0
18	1.722603922541392E-4	0.0	0.0	1.8100260716103678	0.0
19	1.722603922541392E-4	0.0	0.0	1.868422344584521	0.0
20	1.722603922541392E-4	0.0	0.0	1.9360345485442705	0.0
21	1.722603922541392E-4	0.0	0.0	2.020442140748799	0.0
22	1.722603922541392E-4	0.0	0.0	2.112601450604763	0.0
23	1.722603922541392E-4	0.0	0.0	2.2160438161533738	0.0
24	1.722603922541392E-4	0.0	0.0	2.2885654412923664	0.0
25	1.722603922541392E-4	0.0	0.0	2.356091515055989	0.0
26	1.722603922541392E-4	0.0	0.0	2.431972217843937	0.0
27	1.722603922541392E-4	0.0	0.0	2.5141404249491615	0.0
28	1.722603922541392E-4	0.0	0.0	2.589848867344856	0.0
29	1.722603922541392E-4	0.0	0.0	2.679338141120881	0.0
30	1.722603922541392E-4	0.0	0.0	2.7912212658899445	0.0
31	1.722603922541392E-4	0.0	0.0	2.8875148251600082	0.0
32	1.722603922541392E-4	0.0	0.0	2.99621113267237	0.0
33	1.722603922541392E-4	0.0	0.0	3.111281074698135	0.0
34	1.722603922541392E-4	0.0	0.0	3.2319494794721595	0.0
35	1.722603922541392E-4	0.0	0.0	3.3685519705296922	0.0
36	1.722603922541392E-4	0.0	0.0	3.500934081976998	0.0
37	1.722603922541392E-4	0.0	0.0	3.633316193424304	0.0
38	1.722603922541392E-4	0.0	0.0	3.8334827692236138	0.0
39	1.722603922541392E-4	0.0	0.0	4.149580589009959	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12180	0.0	43.89163	1
CGGTCTA	200	0.0	42.750004	31
GTTTTTA	8645	0.0	41.694622	2
CGTTAGA	105	0.0	40.714287	2
CACGACG	205	0.0	40.609753	26
ACGGTCT	215	0.0	39.76744	30
TATACGA	85	0.0	39.705883	2
TGTACGA	150	0.0	39.0	2
CGACGGT	220	0.0	38.863636	28
TTATTTT	9525	0.0	38.858265	6
TATTTTT	9535	0.0	38.841114	7
CGACGTT	145	0.0	38.793102	27
TCGACGT	145	0.0	38.793102	26
ACGGGTG	35	6.248343E-6	38.571426	5
TTTTATT	9675	0.0	38.34884	4
TTTATTT	9715	0.0	38.121464	5
TTTTTAT	9745	0.0	37.842484	3
ACGACGG	220	0.0	37.840908	27
CGCGCGA	30	1.1399289E-4	37.500004	2
TTATACG	30	1.1399289E-4	37.500004	1
>>END_MODULE