FastQCFastQC Report
Thu 26 May 2016
SRR1546943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546943_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1074194
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.6959636713666246No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.3827986378624345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC207.0330786E-445.00000410
CGCAAGT207.0330786E-445.00000429
CTTACGA302.1653686E-645.0000042
CGTATGG253.8906343E-545.02
TAGTCGA351.211829E-745.02
TATGCGA1200.043.1250042
CGTTTTT62250.042.8313261
CGAGATC650.041.538466
GTAACGA603.6379788E-1241.2500042
GCGTATG501.0822987E-940.51
GAGCGAT6500.040.1538437
AGTGCGA900.040.02
ACGTAAG451.9281288E-840.01
GTTTTTA47050.039.21362
TAGAGAT6950.039.172665
GAGGGGA74400.039.0423367
TTATGCG750.039.01
ATGCGAG3350.038.9552233
AGACTTA6500.038.769238
AGGGGAA73750.038.4406788
Produced by FastQC (version 0.11.5)