FastQCFastQC Report
Thu 26 May 2016
SRR1546942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546942_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences821306
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.9318086072669627No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.5051710324775418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA207.0321077E-445.0000047
CTATGCG253.8898295E-545.01
CGCATCG253.8898295E-545.021
GTTAGCG253.8898295E-545.01
CGAATAT253.8898295E-545.014
TAGTCGA253.8898295E-545.02
CGTTTTT61600.043.794641
TATGCGA650.041.538462
ATTGCGA1100.040.9090922
CGGTCTA1050.040.71428731
GTTTTTA44350.040.5862462
CGTAAGA451.9272193E-840.0000042
TATACGA451.9272193E-840.0000042
TGCGTAG451.9272193E-840.0000041
CGACAAT850.039.70588320
TAGAGAC4600.039.1304325
CGTAGGA750.039.02
GAGCGAT5250.039.07
CGACGGT1100.038.86363628
GTCGTAC356.246113E-638.57142630