##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546942_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	821306
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719950907457147	31.0	31.0	34.0	30.0	34.0
2	32.08519358192927	33.0	31.0	34.0	30.0	34.0
3	32.0516250946663	33.0	31.0	34.0	30.0	34.0
4	35.83891387619231	37.0	35.0	37.0	35.0	37.0
5	35.841503653936535	37.0	35.0	37.0	35.0	37.0
6	35.88961726810714	37.0	35.0	37.0	35.0	37.0
7	36.111680664697445	37.0	35.0	37.0	35.0	37.0
8	13.237735996084286	0.0	0.0	36.0	0.0	37.0
9	24.91948433348837	18.0	17.0	39.0	17.0	39.0
10	32.68707521922401	32.0	32.0	38.0	27.0	39.0
11	35.53443418165702	37.0	35.0	37.0	32.0	39.0
12	36.69084360762006	38.0	35.0	39.0	33.0	39.0
13	36.964004889773115	39.0	37.0	39.0	33.0	39.0
14	38.17856925433395	40.0	37.0	41.0	33.0	41.0
15	38.4621493085403	40.0	38.0	41.0	34.0	41.0
16	38.533959328191926	40.0	38.0	41.0	34.0	41.0
17	38.526912015740784	40.0	38.0	41.0	34.0	41.0
18	38.43663871930803	40.0	38.0	41.0	34.0	41.0
19	38.40423788453999	40.0	37.0	41.0	34.0	41.0
20	38.343452988289386	40.0	37.0	41.0	34.0	41.0
21	38.261165753081066	40.0	37.0	41.0	34.0	41.0
22	38.22466291491843	40.0	37.0	41.0	34.0	41.0
23	38.0823079339491	40.0	37.0	41.0	34.0	41.0
24	37.89371074849082	40.0	35.0	41.0	34.0	41.0
25	37.780601870679135	40.0	35.0	41.0	33.0	41.0
26	37.72254068520137	40.0	35.0	41.0	33.0	41.0
27	37.67577370675485	40.0	35.0	41.0	33.0	41.0
28	37.58259162845517	40.0	35.0	41.0	33.0	41.0
29	37.564653125631615	40.0	35.0	41.0	33.0	41.0
30	37.44312107789301	40.0	35.0	41.0	33.0	41.0
31	37.33180203237283	40.0	35.0	41.0	33.0	41.0
32	37.11758224096744	40.0	35.0	41.0	32.0	41.0
33	36.85215741757639	40.0	35.0	41.0	31.0	41.0
34	36.571980479869865	40.0	35.0	41.0	30.0	41.0
35	36.360976298724225	40.0	35.0	41.0	30.0	41.0
36	36.21976827150903	40.0	35.0	41.0	29.0	41.0
37	36.158401375370445	40.0	35.0	41.0	29.0	41.0
38	36.06553951876645	40.0	35.0	41.0	28.0	41.0
39	35.97156723559794	39.0	35.0	41.0	27.0	41.0
40	35.86182859981542	39.0	35.0	41.0	27.0	41.0
41	35.79443106467017	39.0	35.0	41.0	26.0	41.0
42	35.73058518992921	39.0	35.0	41.0	26.0	41.0
43	35.64792172466778	39.0	35.0	41.0	26.0	41.0
44	35.489306056451554	39.0	35.0	40.0	25.0	41.0
45	35.41169064879594	39.0	35.0	40.0	24.0	41.0
46	35.37232773168588	39.0	35.0	40.0	24.0	41.0
47	35.348191782356395	39.0	35.0	40.0	24.0	41.0
48	35.28694664351654	38.0	35.0	40.0	24.0	41.0
49	35.20202579793646	38.0	35.0	40.0	24.0	41.0
50	35.097102663319156	38.0	34.0	40.0	24.0	41.0
51	34.735963209814614	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	8.0
15	19.0
16	20.0
17	101.0
18	255.0
19	506.0
20	974.0
21	1712.0
22	2789.0
23	4473.0
24	6801.0
25	10641.0
26	13784.0
27	14832.0
28	14078.0
29	14275.0
30	16211.0
31	19713.0
32	25454.0
33	35243.0
34	52666.0
35	79765.0
36	84906.0
37	145687.0
38	191615.0
39	84752.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.24827287271735	22.838016524900585	26.96656301061967	17.947147591762388
2	35.31619152910121	23.913376987383508	28.170986209768344	12.599445273746934
3	28.088800033117984	24.4597019868356	34.914879472449975	12.536618507596438
4	25.34049428592997	26.72450950072202	33.7998261305774	14.135170082770612
5	22.486381446135788	30.24512179382593	33.14416307685564	14.124333683182638
6	21.130857439249194	38.7966239136205	30.1945925148483	9.877926132282
7	83.22744506919467	4.040150686832923	10.975324665837094	1.7570795781353115
8	31.384039566251797	63.504223760693336	4.208054001797137	0.9036826712577285
9	79.60893990790278	5.585372565158418	11.888991435591606	2.9166960913471955
10	41.5333627174281	30.291389567347615	17.787280258515096	10.387967456709193
11	32.7555137792735	22.79722782982226	28.698804099811763	15.748454291092479
12	30.924527520802236	21.59231760147862	31.240487711035836	16.242667166683304
13	22.65136258593021	28.404151436857884	31.225876835186885	17.71860914202502
14	17.481304166778276	31.77853321417352	32.75746189605336	17.982700722994842
15	16.447341185867387	25.79111804857142	40.95245864513348	16.80908212042771
16	20.53741236518423	22.436339196353124	39.392017104465324	17.634231333997317
17	20.30558646838085	22.430494846013545	31.50932782665657	25.75459085894904
18	21.782137230216264	24.837880156725994	34.74454101151094	18.635441601546805
19	24.829478903112847	25.765670773134495	30.153316790575012	19.251533533177646
20	26.661804491870267	24.89206215466586	29.848314757228124	18.59781859623575
21	24.162857692504378	25.148117753918758	32.21296325608238	18.47606129749448
22	22.459351325815227	22.78468682805191	32.46780128234787	22.288160563784995
23	20.332129559506445	26.011742273890608	31.96360430826026	21.69252385834269
24	20.278556348060285	24.555281466347502	36.53900008035982	18.627162105232397
25	19.971606197933536	25.930530155630176	33.901858746922585	20.1960048995137
26	19.554830964342155	29.859272914114836	30.56546524681422	20.02043087472879
27	18.975022707736215	28.423510847357747	33.44246845877176	19.15899798613428
28	17.5772489181864	27.031946680043735	36.746718032012424	18.644086369757435
29	18.91913610761397	24.92457135342978	35.906470913398906	20.24982162555734
30	19.96186561403423	26.345113757844214	34.11395996132988	19.57906066679167
31	24.85663078073215	25.47759300431264	30.291633081945097	19.374143133010108
32	25.036101039076787	26.10293849064782	30.334248136504545	18.52671233377085
33	23.81706696407916	26.577426683842564	29.580935729192287	20.02457062288599
34	20.830603940553218	27.0457052548015	31.073680211760298	21.05001059288499
35	20.5057554675115	27.018553377182197	31.29832242793794	21.17736872736836
36	24.702120768629477	26.831168894419378	29.243180982483995	19.223529354467153
37	21.82341295448956	29.26570608275113	29.56279389167984	19.348087071079476
38	20.779587632380622	30.019992548453317	27.576688834612188	21.62373098455387
39	21.015309762743726	28.335115048471582	28.83346767221961	21.81610751656508
40	22.343681892011016	26.681772688863838	29.31501778874135	21.659527630383803
41	19.455964037764247	26.399661027680306	30.315984541693354	23.8283903928621
42	21.59304814527107	26.988601081691844	28.305893296773675	23.112457476263415
43	21.73952217565682	27.057515712779402	28.49376480873146	22.70919730283232
44	20.509529943772478	28.62672377895693	29.464048722400666	21.399697554869928
45	20.437327865618904	30.83783632409845	27.32526001271146	21.399575797571185
46	21.90961712199838	29.112900672830833	29.048612819095442	19.92886938607535
47	21.355499655426843	27.864523088836563	29.62659471622026	21.15338253951633
48	21.434763656907414	26.186342240285594	31.134802375728416	21.24409172707858
49	20.99083654569673	26.001758175393825	31.532948742612376	21.47445653629707
50	20.071447182901377	28.50314012073454	30.62476105130122	20.800651645062864
51	19.597933048096568	29.30125921398358	29.60224325647201	21.49856448144784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	589.0
1	1047.0
2	1505.0
3	10554.5
4	19604.0
5	13426.5
6	7249.0
7	7183.0
8	7117.0
9	7121.0
10	7125.0
11	6879.5
12	6634.0
13	6352.5
14	6071.0
15	5700.0
16	5329.0
17	4931.0
18	4533.0
19	4504.5
20	4476.0
21	4884.5
22	5293.0
23	5804.5
24	6316.0
25	6931.0
26	9193.0
27	10840.0
28	12004.0
29	13168.0
30	15275.5
31	17383.0
32	20113.0
33	22843.0
34	24878.0
35	26913.0
36	28857.0
37	30801.0
38	36176.5
39	41552.0
40	47722.0
41	53892.0
42	57606.5
43	61321.0
44	62646.0
45	63971.0
46	63176.0
47	62381.0
48	60315.5
49	58250.0
50	54893.5
51	51537.0
52	47908.5
53	44280.0
54	41495.5
55	38711.0
56	36635.0
57	34559.0
58	31861.5
59	29164.0
60	26231.5
61	23299.0
62	20196.0
63	17093.0
64	14248.0
65	11403.0
66	9378.0
67	7353.0
68	6080.0
69	4807.0
70	3866.0
71	2925.0
72	2312.5
73	1700.0
74	1283.5
75	673.0
76	479.0
77	360.0
78	241.0
79	172.0
80	103.0
81	81.0
82	59.0
83	36.5
84	14.0
85	8.5
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.322742338284925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04017671759085	21.992451006940623
2	7.251538062503739	4.687791927011485
3	4.278185707725028	4.148480829183876
4	3.0757794210873604	3.9767050286882375
5	2.4589705107189364	3.9740335117704544
6	2.0747223034117668	4.0236428662002925
7	1.8195847111830132	4.11697774475758
8	1.5216387049998437	3.93468286349372
9	1.3128356103766052	3.819100245005523
>10	8.113717421118434	42.153973943919496
>50	0.03624057486375803	0.7889830904074308
>100	0.015477737073810264	0.8691500364699796
>500	3.7750578228786023E-4	0.07394425412054535
>1k	3.7750578228786023E-4	0.5062618982609615
>5k	3.7750578228786023E-4	0.9338207537698574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7653	0.9318086072669627	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4149	0.5051710324775418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0074271952232176554	0.0
2	0.0	0.0	0.0	0.02276861486461806	0.0
3	0.0	0.0	0.0	0.037744762609794646	0.0
4	0.0	0.0	0.0	0.057104173109657055	0.0
5	0.0	0.0	0.0	0.1183480943765174	0.0
6	0.0	0.0	0.0	0.20272590240421962	0.0
7	0.0	0.0	0.0	0.25629911385037973	0.0
8	0.0	0.0	0.0	0.3399463780856343	0.0
9	0.0	0.0	0.0	0.4811848446255111	0.0
10	0.0	0.0	0.0	0.6565153548129442	0.0
11	0.0	0.0	0.0	0.9307127915782911	0.0
12	0.0	0.0	0.0	1.0769433073665602	0.0
13	0.0	0.0	0.0	1.1602252997055909	0.0
14	0.0	0.0	0.0	1.1949261298468536	0.0
15	0.0	0.0	0.0	1.2352277957302151	0.0
16	0.0	0.0	0.0	1.311934893937217	0.0
17	0.0	0.0	0.0	1.3950951289775066	0.0
18	0.0	0.0	0.0	1.5124691649640938	0.0
19	0.0	0.0	0.0	1.5755394457120733	0.0
20	0.0	0.0	0.0	1.6492026114505434	0.0
21	0.0	0.0	0.0	1.73784192493419	0.0
22	0.0	0.0	0.0	1.830012200081334	0.0
23	0.0	0.0	0.0	1.9382544386623257	0.0
24	0.0	0.0	0.0	2.0132569346869498	0.0
25	0.0	0.0	0.0	2.087772401516609	0.0
26	0.0	0.0	0.0	2.1571740617991346	0.0
27	0.0	0.0	0.0	2.2281585669652966	0.0
28	0.0	0.0	0.0	2.3105882582131385	0.0
29	0.0	0.0	0.0	2.3965489111244773	0.0
30	0.0	0.0	0.0	2.5063739945891057	0.0
31	0.0	0.0	0.0	2.600127114619886	0.0
32	0.0	0.0	0.0	2.691201574078358	0.0
33	0.0	0.0	0.0	2.778623314574592	0.0
34	0.0	0.0	0.0	2.878464299542436	0.0
35	0.0	0.0	0.0	2.9887764122020295	0.0
36	0.0	0.0	0.0	3.087034552286237	0.0
37	0.0	0.0	0.0	3.1894324405276473	0.0
38	0.0	0.0	0.0	3.301692669967101	0.0
39	0.0	0.0	0.0	3.4342863682963474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATA	20	7.0321077E-4	45.000004	7
CTATGCG	25	3.8898295E-5	45.0	1
CGCATCG	25	3.8898295E-5	45.0	21
GTTAGCG	25	3.8898295E-5	45.0	1
CGAATAT	25	3.8898295E-5	45.0	14
TAGTCGA	25	3.8898295E-5	45.0	2
CGTTTTT	6160	0.0	43.79464	1
TATGCGA	65	0.0	41.53846	2
ATTGCGA	110	0.0	40.909092	2
CGGTCTA	105	0.0	40.714287	31
GTTTTTA	4435	0.0	40.586246	2
CGTAAGA	45	1.9272193E-8	40.000004	2
TATACGA	45	1.9272193E-8	40.000004	2
TGCGTAG	45	1.9272193E-8	40.000004	1
CGACAAT	85	0.0	39.705883	20
TAGAGAC	460	0.0	39.130432	5
CGTAGGA	75	0.0	39.0	2
GAGCGAT	525	0.0	39.0	7
CGACGGT	110	0.0	38.863636	28
GTCGTAC	35	6.246113E-6	38.571426	30
>>END_MODULE