FastQCFastQC Report
Thu 26 May 2016
SRR1546941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546941_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences840513
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82870.9859454880531294No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.5269400949182226No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9770.11623853527548057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.032203E-445.01
CTATACG207.032203E-445.01
ATTAACG253.889908E-545.01
TAACGCC502.1827873E-1145.012
TCTAACG207.032203E-445.01
ATAACGC502.1827873E-1145.011
GTAACGA253.889908E-545.02
CGTTTTT65350.043.62281
CGGTCTA1300.041.53846431
GTTTTTA48250.041.409332
GTCGATG501.0804797E-940.51
TTAACGA501.0804797E-940.52
TGTACGA451.9272193E-840.0000042
CACGACG1300.039.80769326
GAGCGAT5700.039.4736867
CGAGACT800.039.3756
TATACGA403.4567347E-739.3752
AGGGGAA47600.038.6659668
ATATGCG356.246286E-638.5714261
GTTACGA356.246286E-638.5714262
Produced by FastQC (version 0.11.5)