##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546941_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	840513
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711408389876183	31.0	31.0	34.0	30.0	34.0
2	32.085623898738035	33.0	31.0	34.0	30.0	34.0
3	32.05112116052934	33.0	31.0	34.0	30.0	34.0
4	35.83600967504369	37.0	35.0	37.0	35.0	37.0
5	35.83069506360996	37.0	35.0	37.0	35.0	37.0
6	35.871806860810004	37.0	35.0	37.0	35.0	37.0
7	36.087113465228974	37.0	35.0	37.0	35.0	37.0
8	13.032668144335661	0.0	0.0	35.0	0.0	37.0
9	24.79681337468903	18.0	17.0	38.0	17.0	39.0
10	32.618210545226546	32.0	32.0	37.0	27.0	39.0
11	35.5395324046148	37.0	35.0	37.0	32.0	39.0
12	36.66510095620175	37.0	35.0	39.0	33.0	39.0
13	36.922317679797935	39.0	37.0	39.0	33.0	39.0
14	38.11676202509658	40.0	37.0	41.0	33.0	41.0
15	38.40054466736386	40.0	38.0	41.0	34.0	41.0
16	38.47801878138708	40.0	38.0	41.0	34.0	41.0
17	38.452191697213486	40.0	38.0	41.0	34.0	41.0
18	38.346380127374594	40.0	37.0	41.0	34.0	41.0
19	38.30786317403776	40.0	37.0	41.0	34.0	41.0
20	38.2415774651909	40.0	37.0	41.0	34.0	41.0
21	38.195314052251426	40.0	37.0	41.0	34.0	41.0
22	38.14220125090272	40.0	37.0	41.0	34.0	41.0
23	37.99126485848524	40.0	36.0	41.0	34.0	41.0
24	37.786924176068666	40.0	35.0	41.0	33.0	41.0
25	37.68818566756255	40.0	35.0	41.0	33.0	41.0
26	37.60932906451179	40.0	35.0	41.0	33.0	41.0
27	37.56052434644081	40.0	35.0	41.0	33.0	41.0
28	37.46862570834716	40.0	35.0	41.0	33.0	41.0
29	37.44781460845936	40.0	35.0	41.0	33.0	41.0
30	37.313932086713706	40.0	35.0	41.0	33.0	41.0
31	37.204828479749864	40.0	35.0	41.0	32.0	41.0
32	36.97407654610934	40.0	35.0	41.0	31.0	41.0
33	36.72413990027519	40.0	35.0	41.0	31.0	41.0
34	36.438846276024286	40.0	35.0	41.0	30.0	41.0
35	36.21157317019487	40.0	35.0	41.0	29.0	41.0
36	36.06741240171181	39.0	35.0	41.0	28.0	41.0
37	35.99271278374041	39.0	35.0	41.0	28.0	41.0
38	35.88602317870158	39.0	35.0	41.0	27.0	41.0
39	35.7868087703581	39.0	35.0	41.0	27.0	41.0
40	35.67927444310796	39.0	35.0	41.0	26.0	41.0
41	35.60624285406651	39.0	35.0	41.0	26.0	41.0
42	35.54601772964844	39.0	35.0	41.0	25.0	41.0
43	35.45011439442341	39.0	35.0	41.0	25.0	41.0
44	35.272932125975444	39.0	35.0	40.0	24.0	41.0
45	35.18168903990777	38.0	35.0	40.0	24.0	41.0
46	35.16093980699882	38.0	35.0	40.0	24.0	41.0
47	35.112779933207456	38.0	35.0	40.0	24.0	41.0
48	35.033531902540474	38.0	34.0	40.0	23.0	41.0
49	34.94192475309722	38.0	34.0	40.0	24.0	41.0
50	34.83383719228614	38.0	34.0	40.0	24.0	41.0
51	34.48058626100965	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	5.0
14	7.0
15	26.0
16	55.0
17	146.0
18	296.0
19	589.0
20	1165.0
21	1868.0
22	3122.0
23	4651.0
24	7387.0
25	11159.0
26	14561.0
27	15424.0
28	15033.0
29	15345.0
30	17491.0
31	21130.0
32	27530.0
33	37370.0
34	55938.0
35	83896.0
36	89681.0
37	149301.0
38	187496.0
39	79816.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.609774030859725	22.96740205089035	27.516885521104374	19.905938397145555
2	35.85714914581928	24.466724488496904	26.953063188790654	12.72306317689316
3	28.1309152862597	24.280885602007345	34.227430152775746	13.360768958957209
4	26.13606214300076	25.763313595387576	33.584370497541386	14.516253764070274
5	23.061630218687874	29.529227983386335	32.55380939973564	14.855332398190152
6	21.229653794765817	38.14943968742899	30.29173849779837	10.32916802000683
7	81.5449612320095	4.337470092669596	11.98839280296676	2.129175872354146
8	30.137309000574646	64.4255353575733	4.32747619608501	1.1096794457670494
9	77.68160635231102	5.52626788639795	12.967556718337491	3.8245690429535295
10	42.1590147921567	26.23124211047301	19.050746389407422	12.558996707962875
11	34.67691754916343	22.888759602766406	27.2250399458426	15.209282902227569
12	31.385832223891835	21.06487347607949	31.395588170557744	16.15370612947093
13	23.213442266806105	27.55840778191414	31.871844932796993	17.35630501848276
14	17.88562461258779	30.905411338075677	33.50549009949876	17.70347394983778
15	17.388428257504643	23.684821055712405	41.6575353385373	17.269215348245652
16	20.947564166169947	22.28282013484622	39.0343754350022	17.735240263981638
17	21.8423748353684	21.628695808393207	31.028788370911574	25.50014098532682
18	22.61214282230019	23.86911326773054	33.903342363532744	19.615401546436523
19	26.318807680547472	25.241727373639673	29.42809926794708	19.01136567786578
20	26.925104073345686	24.718713452379678	29.43381006599541	18.922372408279227
21	25.00425335479642	24.670766543765534	31.87886445539807	18.446115646039978
22	23.101486830066875	22.315419273705466	32.35809559162083	22.22499830460683
23	21.673311418145826	25.304665127130693	31.59594200208682	21.426081452636662
24	21.615965487743793	24.12812175421439	35.03455627694039	19.221356481101424
25	21.148274922577045	24.48373790768257	33.78293970468035	20.58504746506003
26	19.512131281729133	27.97172679066237	31.0769732294444	21.439168698164096
27	19.44050835620627	27.17566533771637	33.391869013328765	19.991957292748594
28	18.244215140039476	25.843145793104927	36.88568766931624	19.02695139753936
29	19.267161840447443	25.0529141131666	35.73769828664161	19.942225759744346
30	20.784092572036364	25.544518645160753	34.24468152188009	19.426707260922793
31	25.800671732620433	24.288143074527106	30.101378562853874	19.809806629998583
32	25.948081707243077	25.713820012302012	29.740646486134064	18.59745179432085
33	24.961779294311924	24.853155156434227	29.685918004837518	20.499147544416328
34	21.07272582339595	26.18055877779404	31.229975027156037	21.516740371653977
35	21.079269446159667	26.183890076655565	31.40974619071924	21.327094286465528
36	25.72595545815472	26.007688161872572	29.357547117058274	18.908809262914435
37	21.767896510821366	28.59051555419131	29.412037648436133	20.22955028655119
38	22.099241772584126	29.376702085512065	27.146397497718656	21.377658644185157
39	21.439287673123438	27.889872018636236	28.53697682248817	22.133863485752155
40	22.818564376755624	26.159619184950145	28.958624078390223	22.06319235990401
41	19.97530079844095	25.459570524191772	30.03725105976945	24.527877617597824
42	22.010724402834935	26.780787447665887	28.335314266406346	22.873173883092825
43	22.227615753712314	26.437187765091082	27.96744369212612	23.367752789070483
44	21.259635484519574	27.563880630043798	29.772174850359246	21.404309035077386
45	20.842033377235094	29.258084051049778	27.94079330123389	21.95908927048124
46	22.980727246336464	28.012059301878732	28.87665033140475	20.130563120380053
47	21.740175345295075	27.09000336699135	29.642016244840946	21.52780504287263
48	22.084488877625926	26.037669851626326	30.7835809797112	21.094260291036544
49	21.8730703748782	24.77332295871688	31.149785904560666	22.203820761844252
50	20.266670473865368	28.052034888217076	30.210240650650256	21.4710539872673
51	19.95971507876737	28.705683314832726	29.14315424032704	22.191447366072865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	771.0
1	1353.0
2	1935.0
3	11586.5
4	21238.0
5	14252.0
6	7266.0
7	7230.0
8	7194.0
9	7275.5
10	7357.0
11	7080.0
12	6803.0
13	6646.5
14	6490.0
15	6034.5
16	5579.0
17	5280.5
18	4982.0
19	4745.5
20	4509.0
21	4751.0
22	4993.0
23	5203.5
24	5414.0
25	6556.5
26	8628.0
27	9557.0
28	11079.5
29	12602.0
30	14173.0
31	15744.0
32	17389.0
33	19034.0
34	21143.5
35	23253.0
36	25649.0
37	28045.0
38	33516.5
39	38988.0
40	45683.0
41	52378.0
42	55503.5
43	58629.0
44	59408.0
45	60187.0
46	61468.5
47	62750.0
48	61053.5
49	59357.0
50	57026.5
51	54696.0
52	51025.0
53	47354.0
54	44924.5
55	42495.0
56	40711.5
57	38928.0
58	36445.0
59	33962.0
60	30733.0
61	27504.0
62	24245.0
63	20986.0
64	17832.5
65	14679.0
66	12731.0
67	10783.0
68	8842.0
69	6901.0
70	5578.5
71	4256.0
72	3396.0
73	2536.0
74	1969.0
75	1087.0
76	772.0
77	543.5
78	315.0
79	207.0
80	99.0
81	67.5
82	36.0
83	29.5
84	23.0
85	20.0
86	17.0
87	12.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.987273839448072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.86369032906829	22.347489939903074
2	6.861482613809762	4.389602466250895
3	3.7652135511492943	3.6131675077383956
4	2.765455679005439	3.538375523808152
5	2.1686691263247773	3.4684906605453625
6	1.8665508622326286	3.58235241392958
7	1.6141130383752387	3.614175303643262
8	1.4094783724223359	3.6068296535562268
9	1.229102482489637	3.53840739157273
>10	8.386096511810786	44.50952285781721
>50	0.05037183343201229	1.0643847660106298
>100	0.0182831017756949	1.0308463670458334
>500	7.46249052068903E-4	0.1786704887654597
>1k	3.731245260344515E-4	0.5286116197342826
>5k	3.731245260344515E-4	0.9890730396789345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8287	0.9859454880531294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4429	0.5269400949182226	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	977	0.11623853527548057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.379499186806153E-4	0.0	0.0	0.0035692487802092294	0.0
2	2.379499186806153E-4	0.0	0.0	0.016299569429622147	0.0
3	2.379499186806153E-4	0.0	0.0	0.032599138859244293	0.0
4	2.379499186806153E-4	0.0	0.0	0.060439279344876286	0.0
5	2.379499186806153E-4	0.0	0.0	0.1334899043798252	0.0
6	2.379499186806153E-4	0.0	0.0	0.21831905038946453	0.0
7	2.379499186806153E-4	0.0	0.0	0.274951131035451	0.0
8	2.379499186806153E-4	0.0	0.0	0.3608510516791531	0.0
9	2.379499186806153E-4	0.0	0.0	0.5088559010984958	0.0
10	2.379499186806153E-4	0.0	0.0	0.7659607882329006	0.0
11	2.379499186806153E-4	0.0	0.0	0.9608417716323245	0.0
12	2.379499186806153E-4	0.0	0.0	1.13692471145598	0.0
13	2.379499186806153E-4	0.0	0.0	1.2118789358403737	0.0
14	2.379499186806153E-4	0.0	0.0	1.249475022991911	0.0
15	2.379499186806153E-4	0.0	0.0	1.2883798346961914	0.0
16	2.379499186806153E-4	0.0	0.0	1.3605976350157583	0.0
17	2.379499186806153E-4	0.0	0.0	1.4330533852540055	0.0
18	2.379499186806153E-4	0.0	0.0	1.549172945570146	0.0
19	2.379499186806153E-4	0.0	0.0	1.6175835471908226	0.0
20	2.379499186806153E-4	0.0	0.0	1.690396222307091	0.0
21	2.379499186806153E-4	0.0	0.0	1.7772479426255157	0.0
22	2.379499186806153E-4	0.0	0.0	1.8598165644076892	0.0
23	2.379499186806153E-4	0.0	0.0	1.954877556920595	0.0
24	2.379499186806153E-4	0.0	0.0	2.034828729597282	0.0
25	2.379499186806153E-4	0.0	0.0	2.097647508128964	0.0
26	2.379499186806153E-4	0.0	0.0	2.168318633977107	0.0
27	2.379499186806153E-4	0.0	0.0	2.248388781613134	0.0
28	2.379499186806153E-4	0.0	0.0	2.3283399542898207	0.0
29	2.379499186806153E-4	0.0	0.0	2.4111465259906746	0.0
30	2.379499186806153E-4	0.0	0.0	2.511799341592575	0.0
31	2.379499186806153E-4	0.0	0.0	2.605789559471418	0.0
32	2.379499186806153E-4	0.0	0.0	2.6957346287326907	0.0
33	2.379499186806153E-4	0.0	0.0	2.7863935477500053	0.0
34	2.379499186806153E-4	0.0	0.0	2.8893068875793713	0.0
35	2.379499186806153E-4	0.0	0.0	2.999120775050475	0.0
36	2.379499186806153E-4	0.0	0.0	3.103461814391925	0.0
37	2.379499186806153E-4	0.0	0.0	3.2122049272289663	0.0
38	2.379499186806153E-4	0.0	0.0	3.3418876329099017	0.0
39	2.379499186806153E-4	0.0	0.0	3.528559344114844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.032203E-4	45.0	1
CTATACG	20	7.032203E-4	45.0	1
ATTAACG	25	3.889908E-5	45.0	1
TAACGCC	50	2.1827873E-11	45.0	12
TCTAACG	20	7.032203E-4	45.0	1
ATAACGC	50	2.1827873E-11	45.0	11
GTAACGA	25	3.889908E-5	45.0	2
CGTTTTT	6535	0.0	43.6228	1
CGGTCTA	130	0.0	41.538464	31
GTTTTTA	4825	0.0	41.40933	2
GTCGATG	50	1.0804797E-9	40.5	1
TTAACGA	50	1.0804797E-9	40.5	2
TGTACGA	45	1.9272193E-8	40.000004	2
CACGACG	130	0.0	39.807693	26
GAGCGAT	570	0.0	39.473686	7
CGAGACT	80	0.0	39.375	6
TATACGA	40	3.4567347E-7	39.375	2
AGGGGAA	4760	0.0	38.665966	8
ATATGCG	35	6.246286E-6	38.571426	1
GTTACGA	35	6.246286E-6	38.571426	2
>>END_MODULE