FastQCFastQC Report
Thu 26 May 2016
SRR1546940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546940_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1316255
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.2609676696384819No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21380.16243053207775088No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21220.1612149621463926No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.14184181636536994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA1500.045.0000042
ACTACAT253.891115E-545.00000416
AACGAGT253.891115E-545.0000044
TACTCGC253.891115E-545.00000445
CGTAAGA750.045.0000042
GTAAGCG900.045.0000041
CGATCGA253.891115E-545.0000046
ATCGATC253.891115E-545.00000424
GTACGTA253.891115E-545.00000422
ACGGTTT253.891115E-545.00000411
AACGTGT207.03366E-445.022
AGCGTAA207.03366E-445.019
ACTATCG207.03366E-445.013
CGAACGC207.03366E-445.024
CAACGTA207.03366E-445.035
CGACGAA207.03366E-445.010
ACGTTAG1100.045.01
CGCGATC207.03366E-445.030
TAAGTCG207.03366E-445.038
TGCGGTA207.03366E-445.033