FastQCFastQC Report
Thu 26 May 2016
SRR1546938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546938_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences587359
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.48147725666926017No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.2562317083759677No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6550.111516125572265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG253.8884675E-545.0000041
GCAACGA406.8048394E-945.0000042
TACGTCG207.0304656E-445.0000041
GAGCGAT5250.042.8571437
TCACGAC900.042.525
ACGGTCT900.042.530
CGTTTTT24550.041.883911
CGCGTAA650.041.5384631
GAGGGGA50550.040.504457
CGCATCG501.0804797E-940.50000421
ATTACGA501.0804797E-940.5000042
AGGGGAA51150.040.161298
TTTACGG451.9255822E-840.02
GGCACGA451.9255822E-840.02
GCGATAT451.9255822E-840.09
GCGAGTT451.9255822E-840.05
TGCAACG403.45437E-739.3750041
GACGAGG1900.039.0789454
CCGCTCG700.038.57142619
CGAGGTA356.2430736E-638.5714266