##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546938_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	587359
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843267916214785	31.0	31.0	34.0	30.0	34.0
2	32.2019514470707	33.0	31.0	34.0	30.0	34.0
3	32.21335333245936	33.0	31.0	34.0	30.0	34.0
4	35.93838180737845	37.0	35.0	37.0	35.0	37.0
5	35.93567988232069	37.0	35.0	37.0	35.0	37.0
6	35.98411193154442	37.0	35.0	37.0	35.0	37.0
7	36.17933495528288	37.0	35.0	37.0	35.0	37.0
8	13.18514571156652	0.0	0.0	37.0	0.0	37.0
9	24.93069485612717	18.0	17.0	39.0	17.0	39.0
10	32.80849531547146	32.0	32.0	38.0	27.0	39.0
11	35.688558445516286	37.0	35.0	37.0	32.0	39.0
12	36.73440604468477	38.0	35.0	39.0	33.0	39.0
13	36.934501727223044	39.0	37.0	39.0	33.0	39.0
14	38.13229728326288	40.0	37.0	41.0	33.0	41.0
15	38.43694401549989	40.0	38.0	41.0	34.0	41.0
16	38.537667763667535	40.0	38.0	41.0	34.0	41.0
17	38.52764323011991	40.0	38.0	41.0	34.0	41.0
18	38.46164951928888	40.0	37.0	41.0	34.0	41.0
19	38.44032354999242	40.0	37.0	41.0	34.0	41.0
20	38.41300124795909	40.0	37.0	41.0	34.0	41.0
21	38.33848293803279	40.0	37.0	41.0	34.0	41.0
22	38.28767244564227	40.0	37.0	41.0	34.0	41.0
23	38.18040925566817	40.0	37.0	41.0	34.0	41.0
24	38.07350019323787	40.0	36.0	41.0	34.0	41.0
25	37.976173004925435	40.0	36.0	41.0	34.0	41.0
26	37.92172759760215	40.0	36.0	41.0	34.0	41.0
27	37.86655180221977	40.0	36.0	41.0	33.0	41.0
28	37.79946676564077	40.0	36.0	41.0	33.0	41.0
29	37.8149121746666	40.0	36.0	41.0	33.0	41.0
30	37.71707422547369	40.0	36.0	41.0	33.0	41.0
31	37.63118637834783	40.0	36.0	41.0	33.0	41.0
32	37.47785936709917	40.0	35.0	41.0	33.0	41.0
33	37.326820564595074	40.0	35.0	41.0	33.0	41.0
34	37.15178281085333	40.0	35.0	41.0	32.0	41.0
35	37.012200374898484	40.0	35.0	41.0	32.0	41.0
36	36.88780115738415	40.0	35.0	41.0	31.0	41.0
37	36.812320914466284	40.0	35.0	41.0	31.0	41.0
38	36.72687572677017	40.0	35.0	41.0	31.0	41.0
39	36.654143377389296	40.0	35.0	41.0	31.0	41.0
40	36.53780056149646	39.0	35.0	41.0	30.0	41.0
41	36.50110409476998	39.0	35.0	41.0	31.0	41.0
42	36.44835100849736	39.0	35.0	41.0	30.0	41.0
43	36.35048241365162	39.0	35.0	41.0	30.0	41.0
44	36.15112903692631	39.0	35.0	41.0	30.0	41.0
45	36.06862242682925	39.0	35.0	40.0	30.0	41.0
46	36.0405544139104	39.0	35.0	40.0	30.0	41.0
47	36.012280394103094	39.0	35.0	40.0	30.0	41.0
48	35.9474120597454	39.0	35.0	40.0	29.0	41.0
49	35.85813276037313	38.0	35.0	40.0	29.0	41.0
50	35.74318091661148	38.0	35.0	40.0	29.0	41.0
51	35.36980279522405	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	4.0
15	8.0
16	24.0
17	65.0
18	146.0
19	276.0
20	516.0
21	865.0
22	1442.0
23	2248.0
24	3573.0
25	5233.0
26	7002.0
27	7407.0
28	7649.0
29	8256.0
30	9961.0
31	13104.0
32	17752.0
33	25137.0
34	39728.0
35	62888.0
36	62242.0
37	107727.0
38	144950.0
39	59136.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.31189442913108	22.89655900394818	28.341440243530787	14.450106323389955
2	33.12812096179679	24.254331677900566	29.78723404255319	12.83031331774945
3	29.902155240662015	24.181292871991406	32.409480402956284	13.507071484390295
4	27.270715184410214	26.401229912200204	31.25277726228763	15.07527764110195
5	23.264987852403728	32.2038140217482	30.116334303211495	14.414863822636582
6	21.933434236982833	39.99223643461664	27.763429180450117	10.3109001479504
7	85.61339827941686	4.549347162467929	7.993067272315568	1.8441872857996557
8	32.11255807776845	63.99935984636313	2.8571963654255748	1.0308857104428466
9	81.64359446267105	5.7096256292999685	9.326834184885223	3.319945723143767
10	47.13658937719521	25.482711595463766	15.575823303975934	11.804875723365097
11	40.279284049448464	22.38460634807673	22.55826504744117	14.777844555033633
12	35.65008793599826	22.525916858343876	26.72777636845609	15.09621883720178
13	23.58846974337671	32.118516954707424	27.36299265015093	16.930020651764934
14	17.53084570084054	35.2009588684263	30.01435238074159	17.25384304999157
15	16.531286657734025	24.94913672898517	42.35569728224136	16.16387933103945
16	20.51573228638703	21.120983929760165	40.504189090488104	17.859094693364703
17	21.497755205930275	20.752895588558275	28.978018554240254	28.7713306512712
18	24.07965145677516	24.169204864486627	32.689888126341806	19.061255552396407
19	27.422411165913864	25.482371088210105	26.91539586522042	20.179821880655613
20	29.42595584642442	25.116155536903324	26.604342489005873	18.853546127666384
21	25.198217785034366	25.025240100177232	30.397593294731163	19.37894882005724
22	24.30251345429286	23.440689595290102	28.9276234806992	23.32917346971784
23	21.42335437100649	26.87164068312565	28.385706186506038	23.31929875936182
24	21.73474825447469	24.443823964560004	34.58430023205569	19.237127548909612
25	21.000444361966018	25.20656021274893	32.7026571483539	21.090338276931146
26	19.263176353814277	30.928784610434164	28.265847633219206	21.542191402532353
27	19.695790819583934	29.322952402193547	31.872840971194787	19.10841580702773
28	18.06646361084107	27.555719755720098	36.29126309463208	18.08655353880676
29	19.221293961614617	25.962997076745225	34.53799124555851	20.277717716081646
30	20.582301454476735	26.872662204886623	33.19009328196214	19.354943058674507
31	27.29846652558316	25.40984304318143	27.872221247993135	19.419469183242278
32	27.2150422484375	26.66001542497859	28.441549376105584	17.68339295047833
33	25.97099899720614	26.726584593068292	27.694816968838477	19.60759944088709
34	21.362573826228935	27.64748646058033	29.508188348182284	21.481751365008453
35	21.195214511057124	26.413828680585468	30.558312718456687	21.832644089900725
36	27.368100258955767	25.86373921230457	27.82199642807891	18.946164100660752
37	22.302884607199346	30.03512332321459	28.41873539011065	19.243256679475415
38	22.455772364090787	30.649568662436433	25.30275351190669	21.591905461566093
39	21.848647930822544	29.146399391173027	28.041793860313707	20.963158817690715
40	22.623982947396737	26.07808852848088	28.120110528654536	23.177817995467848
41	19.728139008681232	25.145268907090895	29.083916310127194	26.042675774100676
42	22.236996453616953	26.863128001784258	27.40164022344086	23.498235321157928
43	23.103927921424546	26.052039723576208	27.486596783227974	23.35743557177127
44	20.724803740131676	29.847333572823437	28.42179995539355	21.00606273165134
45	20.62282181766177	32.34325174212024	25.420569021671586	21.613357418546407
46	22.88719505447265	29.371304432212668	27.677451098902033	20.06404941441265
47	22.397205116461993	27.965179728241164	27.898099799270977	21.73951535602587
48	23.342112745356758	26.34794052700308	29.402120338668514	20.90782638897165
49	22.1688950028858	25.404394927122937	30.369331192677734	22.057378877313532
50	20.2291954324357	30.162132528828195	28.419927165498443	21.18874487323766
51	20.605796454978982	30.677490257236208	26.874534994781722	21.842178293003087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	512.0
1	844.5
2	1177.0
3	4323.0
4	7469.0
5	5286.0
6	3103.0
7	3134.0
8	3165.0
9	3199.0
10	3233.0
11	3177.5
12	3122.0
13	3067.5
14	3013.0
15	2810.0
16	2607.0
17	2518.0
18	2429.0
19	2486.0
20	2543.0
21	2675.5
22	2808.0
23	3143.5
24	3479.0
25	4394.0
26	6174.5
27	7040.0
28	7952.5
29	8865.0
30	10530.5
31	12196.0
32	13322.5
33	14449.0
34	15893.5
35	17338.0
36	19693.5
37	22049.0
38	26608.5
39	31168.0
40	36587.0
41	42006.0
42	45278.5
43	48551.0
44	48305.0
45	48059.0
46	46985.5
47	45912.0
48	44043.5
49	42175.0
50	39605.0
51	37035.0
52	34719.5
53	32404.0
54	30171.5
55	27939.0
56	26602.0
57	25265.0
58	23816.5
59	22368.0
60	20225.5
61	18083.0
62	16107.0
63	14131.0
64	11866.5
65	9602.0
66	8020.0
67	6438.0
68	5259.5
69	4081.0
70	3407.5
71	2734.0
72	2205.5
73	1677.0
74	1333.5
75	732.5
76	475.0
77	339.0
78	203.0
79	136.0
80	69.0
81	62.0
82	55.0
83	36.0
84	17.0
85	12.0
86	7.0
87	6.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.221305478436058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.11579518990986	18.94095358482596
2	6.348813545792514	3.583436130028867
3	3.6486387800474365	3.0890804877656093
4	2.878473369626071	3.2493710510304217
5	2.313263415758129	3.2641656754100303
6	1.9787878064653726	3.350638509795821
7	1.8211557991633802	3.5976775892410577
8	1.577459965279441	3.5614383648123393
9	1.410771671940774	3.5832436482748107
>10	10.834174247881684	51.26169206230339
>50	0.05510520783592917	0.9901633402815324
>100	0.01574434509597965	0.6757172674271932
>500	6.055517344607558E-4	0.11193596615195031
>1k	0.0012111034689215117	0.7404863226509933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2828	0.48147725666926017	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1505	0.2562317083759677	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	655	0.111516125572265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008512681341394275	0.0
2	0.0	0.0	0.0	0.024005761382731857	0.0
3	0.0	0.0	0.0	0.0328589499777819	0.0
4	0.0	0.0	0.0	0.05056532716788199	0.0
5	0.0	0.0	0.0	0.08785087144318891	0.0
6	0.0	0.0	0.0	0.12666869835994682	0.0
7	0.0	0.0	0.0	0.14829090896708827	0.0
8	0.0	0.0	0.0	0.19170558380819908	0.0
9	0.0	0.0	0.0	0.24788928066140128	0.0
10	0.0	0.0	0.0	0.38187888497494715	0.0
11	0.0	0.0	0.0	0.5514514972955211	0.0
12	0.0	0.0	0.0	0.6849303407285834	0.0
13	0.0	0.0	0.0	0.734474146135498	0.0
14	0.0	0.0	0.0	0.7571178785036068	0.0
15	0.0	0.0	0.0	0.7944034227789137	0.0
16	0.0	0.0	0.0	0.8645479170320026	0.0
17	0.0	0.0	0.0	0.941162049104551	0.0
18	0.0	0.0	0.0	1.0399091526647246	0.0
19	0.0	0.0	0.0	1.096092849517927	0.0
20	0.0	0.0	0.0	1.1630025248612859	0.0
21	0.0	0.0	0.0	1.2418299540825968	0.0
22	0.0	0.0	0.0	1.318954847035629	0.0
23	0.0	0.0	0.0	1.4105512982690314	0.0
24	0.0	0.0	0.0	1.4815470606562597	0.0
25	0.0	0.0	0.0	1.5455624243435446	0.0
26	0.0	0.0	0.0	1.613323367821043	0.0
27	0.0	0.0	0.0	1.684659637461927	0.0
28	0.0	0.0	0.0	1.7541231172077043	0.0
29	0.0	0.0	0.0	1.8310777565339085	0.0
30	0.0	0.0	0.0	1.9172260917088186	0.0
31	0.0	0.0	0.0	2.0023529051227613	0.0
32	0.0	0.0	0.0	2.0808398270904167	0.0
33	0.0	0.0	0.0	2.1584754809239324	0.0
34	0.0	0.0	0.0	2.24241051895008	0.0
35	0.0	0.0	0.0	2.339114578988319	0.0
36	0.0	0.0	0.0	2.4203255589852204	0.0
37	0.0	0.0	0.0	2.5063036405333023	0.0
38	0.0	0.0	0.0	2.6103286065251403	0.0
39	0.0	0.0	0.0	2.7412536455557843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.8884675E-5	45.000004	1
GCAACGA	40	6.8048394E-9	45.000004	2
TACGTCG	20	7.0304656E-4	45.000004	1
GAGCGAT	525	0.0	42.857143	7
TCACGAC	90	0.0	42.5	25
ACGGTCT	90	0.0	42.5	30
CGTTTTT	2455	0.0	41.88391	1
CGCGTAA	65	0.0	41.53846	31
GAGGGGA	5055	0.0	40.50445	7
CGCATCG	50	1.0804797E-9	40.500004	21
ATTACGA	50	1.0804797E-9	40.500004	2
AGGGGAA	5115	0.0	40.16129	8
TTTACGG	45	1.9255822E-8	40.0	2
GGCACGA	45	1.9255822E-8	40.0	2
GCGATAT	45	1.9255822E-8	40.0	9
GCGAGTT	45	1.9255822E-8	40.0	5
TGCAACG	40	3.45437E-7	39.375004	1
GACGAGG	190	0.0	39.078945	4
CCGCTCG	70	0.0	38.571426	19
CGAGGTA	35	6.2430736E-6	38.571426	6
>>END_MODULE